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clean up split-seq
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<h1><a href="#SPLiT-seq" target="_self">SPLiT-seq</a> / <a href="#microSPLiT" target="_self">microSPLiT</a></h1>

<span style="font-size:1.1em;"><p>The <b>SPLiT-seq</b> uses the combinatorial indexing to identify single cells without single cell isolation. Multi-level indexing can be performed by ligations. The workflow described here is based on the publication of <a href="https://www.science.org/doi/10.1126/science.aam8999" target="_blank">Science 360, 176-182 (2018)</a>. To be consistent with the publication, all the oligo/primer names are the same as the Science 2018 publication. All oligos can be found in the <a href="../data/aam8999_tables12.xlsx">Supplementary Table S12</a> from the publication. <b>NOTE:</b> The published version in this page is different from the preprint version in the bioRxiv. I have archived the previous Github Page about SPLiT-seq, which was based on the preprint version. If you want to have a look, you can check by <a href="https://teichlab.github.io/scg_lib_structs/methods_html/SPLiT-seq_archive.html">clicking here</a>.
<p><info>The <b>SPLiT-seq</b> uses the combinatorial indexing to identify single cells without single cell isolation. Multi-level indexing can be performed by ligations. The workflow described here is based on the publication of <a href="https://www.science.org/doi/10.1126/science.aam8999" target="_blank">Science 360, 176-182 (2018)</a>. To be consistent with the publication, all the oligo/primer names are the same as the Science 2018 publication. All oligos can be found in the <a href="../data/SPLiT-seq/aam8999_tables12.xlsx">Supplementary Table S12</a> from the publication. <b>NOTE:</b> The published version in this page is different from the preprint version in the bioRxiv. I have archived the previous Github Page about SPLiT-seq, which was based on the preprint version. If you want to have a look, you can check by <a href="https://teichlab.github.io/scg_lib_structs/methods_html/SPLiT-seq_archive.html">clicking here</a>.</info></p>

<span style="font-size:1.1em;"><p>Be careful that there are different versions of the protocol, and they use slightly different oligo sequences. The basic idea of the method does not change. Check <a href="https://github.com/Teichlab/scg_lib_structs/issues/13" target="_blank">this thread</a> for more information.</p></span>
<p><info>Be careful that there are different versions of the protocol, and they use slightly different oligo sequences. The basic idea of the method does not change. Check <a href="https://github.com/Teichlab/scg_lib_structs/issues/13" target="_blank">this thread</a> for more information.</info></p>

<span style="font-size:1.1em;"><p><b>microSPLiT</b> was based on <b>SPLiT-seq</b>, and it was developed from the same lab that developed SPLiT-seq. <b>microSPLiT</b> was further optimised to work on sequencing mRNA from bacteria. The oligo sequences used in <b>microSPLiT</b> is almost the same as those in <b>SPLiT-seq</b>, except that the Round2 linker region is shorter. Therefore, the final library of <b>microSPLiT</b> is extremely similar to the original <b>SPLiT-seq</b>. The procedures described here is based on the Science paper and the oligo sequences are taken from their <a href="../data/aba5257_table_s3.xlsx", target="_blank">Supplementary Table S3</a>.</p></span>
<p><info><b>microSPLiT</b> was based on <b>SPLiT-seq</b>, and it was developed from the same lab that developed SPLiT-seq. <b>microSPLiT</b> was further optimised to work on sequencing mRNA from bacteria. The oligo sequences used in <b>microSPLiT</b> is almost the same as those in <b>SPLiT-seq</b>, except that the Round2 linker region is shorter. Therefore, the final library of <b>microSPLiT</b> is extremely similar to the original <b>SPLiT-seq</b>. The procedures described here is based on the Science paper and the oligo sequences are taken from their <a href="../data/SPLiT-seq/aba5257_table_s3.xlsx", target="_blank">Supplementary Table S3</a>.</info></p>

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