diff --git a/methods_html/CH-ATAC-seq.html b/methods_html/CH-ATAC-seq.html index 4ecab43..9a4a6f7 100644 --- a/methods_html/CH-ATAC-seq.html +++ b/methods_html/CH-ATAC-seq.html @@ -10,7 +10,7 @@

CH-ATAC-seq

-

CH-ATAC-seq is like sci-ATAC-seq3 that uses combinatorial indexing to achieve single cell chromatin accessibility profiling. They difference is that CH-ATAC-seq uses barcoded Tn5 with to add the first-level barcode. Then subsequent rounds of barcodes are added through hybridisation. The oligo sequences here are taken from the Supplementary Table 1 from the paper, which is designed to work on the BGI system.

+

CH-ATAC-seq is like sci-ATAC-seq3 that uses combinatorial indexing to achieve single cell chromatin accessibility profiling. They difference is that CH-ATAC-seq uses barcoded Tn5 with to add the first-level barcode. Then subsequent rounds of barcodes are added through hybridisation. The oligo sequences here are taken from the Supplementary Table 1 from the paper, which is designed to work on the BGI system.


diff --git a/methods_html/itChIP-seq.html b/methods_html/itChIP-seq.html index 37f5935..085a843 100644 --- a/methods_html/itChIP-seq.html +++ b/methods_html/itChIP-seq.html @@ -48,7 +48,7 @@

Adapter and primer sequences:

Step-by-step library generation

(1) Anneal Barcoded Tn5 sequences s5/s7 and Tn5 binding site 19-bp Mosaic End (ME) bottom strand to assemble the barcoded Tn5 transposome, sort single nulcei into well plates, perform tagmentation to barcode each nulcei:

-Tn5 dimer +Tn5 dimer

(2) Sort limited nulcei into wells, and perform tagmentation using barcoded Tn5 transposome: