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While there isn't currently an elegant way to toggle between offline/online work, you can force tyto to use a local copy of the ontology when one is available by unsetting the endpoints attribute.
tyto.SO.endpoints = []
When no endpoints are available, tyto will fall back to loading a local copy of the ontology if one is available. Currently, ontology files are distributed with tyto for a few of the ontologies (SO, SBO, OM) supporting offline work, but some are not (NCIT is too large for distribution).
Can we give tyto the ability to run offline when somebody is not connected to the internet?
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