From e54fdc6481eb76a2c02cf8aef34f5cfb66339424 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Fri, 9 Dec 2022 13:46:56 -0700 Subject: [PATCH 01/17] Adding additions to temp3 - more work needing to be done. Framework set up. --- temp3.py | 76 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 76 insertions(+) diff --git a/temp3.py b/temp3.py index 0e5c0ad..9abb5dc 100644 --- a/temp3.py +++ b/temp3.py @@ -10,6 +10,10 @@ g = rdflib.Graph() result = g.parse(file_path_in) +# Creating the Sbol document to be changed from the conversion +doc = sbol3.Document() +doc.read(file_path_in) + # collect all the objects which are combinatorial derivations and their featured components knows_query = """ SELECT @@ -34,16 +38,88 @@ else: parent_dict[s] = {o} +parent_dict_copy = parent_dict + +print(parent_dict) + # objects that are only children are objects that are not also parents child_only = children - set(parent_dict).intersection(children) +# Loop through children create a dictionary for the object and the type of the object to look at in while loop +# Keep track of the old uris and new uris in case they change - function already exists to change in document + +old_uris = [] # Correlates index to index with each of the new uris +new_uris = [] + # process the children only objects and update the 'tree' while len(parent_dict) > 0: # do something to deal with child_only # for this you probably need to read in the sbol document at the top and perform actions using pysbol + # Import SBOL document as the SBOL document type + # Go through each of the children and determine if it is a combinatorial derivation - may have to save a copy of the parent dictionary + # Go through each of the uris in the child object and look to see if those uris in parent dictionary are combinatorial derivations - going to need pysbol + # Take it out as a string and check what the type is in the object in the sbol document + # May have to write in another file for now + # Object stays combinatorial derivation if one of its subcomponents is a combinatorial derivation + # Else: Becomes a component + # For collection: If has a variable feature and nothing else + + # loop over parents + + for parent in parent_dict: + if parent_dict[parent].issubset(child_only): # If the parent's children are a subset of childOnly + print(parent) # Print the name of the parent object + child_types = [] # Create a list of all of the types of child objects + for child in parent_dict[parent]: + children_of_parent = set() + obj = doc.find(child) + children_of_parent.add(type(obj)) + + # if len(child_types) == len(parent_dict[parent]): # Check to see that it's the end of the loop + # Trying to see if it can be a collection object + if len(children_of_parent) == 0: # Checking to see if there aren't any children and only contains a variable feature + parentObj = doc.find(parent) + if len(parentObj.variable_features) == 1: # If it only has 1 variable feature and nothing else - collection object + + col = sbol3.Collection() + + + if sbol3.CombinatorialDerivation not in children_of_parent: # If there is no child that is a combinatorial derivation + # Delete the template that's currently in the document + # Create a new component object and carry over all of the features from the combinatorial derivation (including exact name) + # Name, (references to) subcomponents, (references to) constraints, name, displayId, role, type + # Delete the previous combinatorial derivation object + # Put the new component object into the document + + parentObj = doc.find(parent) # Get the object that the parent is supposed to be + + template = doc.find(f'{parent}_template') # Get the template object from the parent + + # print(parentObj._properties) + old_uris.append(parentObj.identity) # Add the uri of the old parent object to the list + + + componentObj = sbol3.Component(identity=parentObj.identity, types=parentObj.type_uri) # Create a new component object + + new_uris.append(componentObj.identity) # Add its URI to the list of new uris + + componentObj.name = parentObj.name # Set the name + + for feature in template.features: + print(feature._properties, '\n') # Test printing out all the properties of the current template + + # componentObj.features = template.features + # Still need to find out how to attribute subcomponents, constraints, roles from combderiv to component + + doc.remove_object(parentObj) # Remove the old object + doc.add(componentObj) # Add the new object + + # print(child, parent_dict_copy[child]) + # remove child only children from dictionary new_parent_dict = {} + for key in parent_dict: current_children = parent_dict[key] remaining_children = current_children - current_children.intersection(child_only) From 63af587c83fd3ea53b094bd1eb946935415d11cf Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 20 Dec 2022 15:10:13 -0700 Subject: [PATCH 02/17] Adding more features to the post-processing tool - Still need to work on finishing the collection conversion and ensuring that every piece of information is passed from one object to another. Also need to incoorporate the tool comparing old uris to new uris in the returned document --- temp3.py | 54 +++++++++++++++++++++++++++++++++++++++++++++++------- 1 file changed, 47 insertions(+), 7 deletions(-) diff --git a/temp3.py b/temp3.py index 9abb5dc..dff1f1a 100644 --- a/temp3.py +++ b/temp3.py @@ -79,10 +79,15 @@ # if len(child_types) == len(parent_dict[parent]): # Check to see that it's the end of the loop # Trying to see if it can be a collection object if len(children_of_parent) == 0: # Checking to see if there aren't any children and only contains a variable feature - parentObj = doc.find(parent) - if len(parentObj.variable_features) == 1: # If it only has 1 variable feature and nothing else - collection object + parentObj = doc.find(parent) # In template not in the parent + template = doc.find(f'{parent}_template') # Get the template object from the parent + + if len(template.features) == 1: # If it only has 1 variable feature and nothing else - collection object + + if type(template.features[0]) == sbol3.VariableFeature: + col = sbol3.Collection() + - col = sbol3.Collection() if sbol3.CombinatorialDerivation not in children_of_parent: # If there is no child that is a combinatorial derivation @@ -99,20 +104,53 @@ # print(parentObj._properties) old_uris.append(parentObj.identity) # Add the uri of the old parent object to the list - componentObj = sbol3.Component(identity=parentObj.identity, types=parentObj.type_uri) # Create a new component object new_uris.append(componentObj.identity) # Add its URI to the list of new uris componentObj.name = parentObj.name # Set the name + print(parentObj._properties) + for feature in template.features: - print(feature._properties, '\n') # Test printing out all the properties of the current template + if type(feature) != sbol3.LocalSubComponent: + subComp = sbol3.SubComponent(instance_of=feature.instance_of) + componentObj.features.append(subComp) # + + for role in template.roles: + componentObj.roles.append(role) + + # Still need to find out how to attribute subcomponents, constraints (Both subcomponents and constraints and roles in template) + for prop in parentObj._properties: + for subProp in parentObj._properties[prop]: + print(prop, type(subProp)) + + if type(subProp) == rdflib.term.URIRef: + setattr(componentObj, prop, + sbol3.URIProperty(componentObj, + f'{prop}', + '0', '*', initial_value=[subProp])) + else: + setattr(componentObj, prop, + sbol3.TextProperty(componentObj, + prop, + '0', '*', initial_value=str(subProp))) + + for constraint in template.constraints: + print(constraint) + newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) + newConstraint.derived_from = constraint.derived_from + componentObj.constraints.append(newConstraint) + # Add all to component + # Eventually should be more filtered + + componentObj.types[0] = sbol3.component.SBOL_COMPONENT + print(componentObj._properties) - # componentObj.features = template.features - # Still need to find out how to attribute subcomponents, constraints, roles from combderiv to component doc.remove_object(parentObj) # Remove the old object + doc.remove_object(template) # Remove the old template + doc.add(componentObj) # Add the new object # print(child, parent_dict_copy[child]) @@ -130,4 +168,6 @@ # update child_only list child_only = children - set(parent_dict).intersection(children) + # Update uris on document using the old uri and new uri comparison + # return updated sbol document \ No newline at end of file From eb9a23e7d896a7ff2eac18a72a7f2fd8c13e281b Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 22 Dec 2022 13:41:04 -0700 Subject: [PATCH 03/17] Removed the template property from components which had their cd /Users/williamdravenstott/Desktop/Code ; /usr/bin/env /Users/williamdravenstott/opt/anaconda3/bin/python /Users/williamdravenstott/.vscode/extensions/ms-python.python-2022.18.2/pythonFiles/lib/python/debugpy/adapter/../../debugpy/launcher 63754 -- /Users/williamdravenstott/Desktop/Code/Excel-utilities/temp3.py q --- temp3.py | 9 ++++++--- 1 file changed, 6 insertions(+), 3 deletions(-) diff --git a/temp3.py b/temp3.py index dff1f1a..e6538b8 100644 --- a/temp3.py +++ b/temp3.py @@ -123,7 +123,8 @@ # Still need to find out how to attribute subcomponents, constraints (Both subcomponents and constraints and roles in template) for prop in parentObj._properties: for subProp in parentObj._properties[prop]: - print(prop, type(subProp)) + if prop == "http://sbols.org/v3#template": + continue if type(subProp) == rdflib.term.URIRef: setattr(componentObj, prop, @@ -137,7 +138,6 @@ '0', '*', initial_value=str(subProp))) for constraint in template.constraints: - print(constraint) newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) newConstraint.derived_from = constraint.derived_from componentObj.constraints.append(newConstraint) @@ -145,6 +145,7 @@ # Eventually should be more filtered componentObj.types[0] = sbol3.component.SBOL_COMPONENT + print(componentObj._properties) @@ -170,4 +171,6 @@ # Update uris on document using the old uri and new uri comparison -# return updated sbol document \ No newline at end of file +# return updated sbol document +file_path_out = "SampleTemp3Output.nt" +doc.write(file_path_out) \ No newline at end of file From 23f2e453a97d8e48c991576e506d29edd7c266f2 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 22 Dec 2022 13:46:44 -0700 Subject: [PATCH 04/17] Adding full support of components, need to finish the uris checker and combdev -> collection feature --- temp3.py | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/temp3.py b/temp3.py index e6538b8..40703cd 100644 --- a/temp3.py +++ b/temp3.py @@ -1,6 +1,7 @@ import rdflib import os import sbol3 +import excel_sbol_utils.helpers as h direct = os.path.split(__file__)[0] file_path_in = os.path.join(direct, 'SBOL3_simple_library4.nt') @@ -169,7 +170,12 @@ # update child_only list child_only = children - set(parent_dict).intersection(children) - # Update uris on document using the old uri and new uri comparison +# Update uris on document using the old uri and new uri comparison +oldToNew = {} +for i in range(len(old_uris)): + oldToNew[old_uris[i]] = new_uris[i] + +# h.update_uri_refs(doc, oldToNew) # Still needs to be completed # return updated sbol document file_path_out = "SampleTemp3Output.nt" From de318d66ac4db2897162d17f3f6b180dbb250a6b Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Fri, 13 Jan 2023 14:01:30 -0700 Subject: [PATCH 05/17] Added collection creation and uri referencing updating --- temp3.py | 16 ++++++++++++---- 1 file changed, 12 insertions(+), 4 deletions(-) diff --git a/temp3.py b/temp3.py index 40703cd..b83ac65 100644 --- a/temp3.py +++ b/temp3.py @@ -83,10 +83,18 @@ parentObj = doc.find(parent) # In template not in the parent template = doc.find(f'{parent}_template') # Get the template object from the parent - if len(template.features) == 1: # If it only has 1 variable feature and nothing else - collection object + if len(template.features) == 1 and type(template.features[0]) == sbol3.VariableFeature: # If it only has 1 variable feature and nothing else - collection object + col = sbol3.Collection() + for variant in template.features[0]: # Add all variants of the variable feature to the collection + col.members.append(variant) + + # Delete original comb der + template after adding in uris to appropriate arrays + old_uris.append(parentObj.identity) + new_uris.append(col.identity) + + doc.remove_object(parentObj) + doc.remove_object(template) - if type(template.features[0]) == sbol3.VariableFeature: - col = sbol3.Collection() @@ -175,7 +183,7 @@ for i in range(len(old_uris)): oldToNew[old_uris[i]] = new_uris[i] -# h.update_uri_refs(doc, oldToNew) # Still needs to be completed +h.update_uri_refs(doc, oldToNew) # return updated sbol document file_path_out = "SampleTemp3Output.nt" From 2fcc9b443ecefb3a4967e4bdc870fcf5cf492a69 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 8 Jun 2023 13:06:51 -0600 Subject: [PATCH 06/17] Updated the check_name function to allow hyphens in the name. --- excelutils/excel_sbol_utils/helpers.py | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/excelutils/excel_sbol_utils/helpers.py b/excelutils/excel_sbol_utils/helpers.py index 254db55..42c5497 100644 --- a/excelutils/excel_sbol_utils/helpers.py +++ b/excelutils/excel_sbol_utils/helpers.py @@ -2,6 +2,7 @@ import string import rdflib from openpyxl.worksheet import cell_range, worksheet +from openpyxl import load_workbook from pathlib import Path def check_name(nm_to_chck): @@ -22,6 +23,9 @@ def check_name(nm_to_chck): for ltr in nm_to_chck: if ord(ltr) == 32: nm_to_chck = nm_to_chck.replace(ltr, "_") + elif ord(ltr) == 45: + # Allow hyphens to be reinterpreted as underscores + nm_to_chck = nm_to_chck.replace(ltr, "_") elif ord(ltr) > 122 or ord(ltr) < 48: # 122 is the highest decimal code number # for common latin ltrs or arabic numbers @@ -124,7 +128,7 @@ def read_variant_table(excel_file: Path) -> tuple[str, str, list[list]]: LAST_VARIANT_ROW = 35 print(f'Loading workbook "{excel_file}"') - work_book = openpyxl.load_workbook(excel_file, data_only=True) + work_book = load_workbook(excel_file, data_only=True) sheet = work_book[VARIANTS_SHEET] # First, get the library name @@ -133,7 +137,7 @@ def read_variant_table(excel_file: Path) -> tuple[str, str, list[list]]: # Then get the base sequence print('Extracting base sequence') - first_aa_column = get_column_number(FIRST_AMINO_ACID_COLUMN) + first_aa_column = col_to_num(FIRST_AMINO_ACID_COLUMN) last_aa_column = row_ends(sheet, ORIGINAL_AMINO_ACID_ROW, first_aa_column) # Get row from sheet and concatenate it into a string row_iterator = sheet.iter_rows(min_row=ORIGINAL_AMINO_ACID_ROW, max_row=ORIGINAL_AMINO_ACID_ROW, From 8ab709185b9fa5d33016800060b7a86ccd80fd82 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Mon, 19 Jun 2023 14:27:22 -0600 Subject: [PATCH 07/17] Update to post-processing of combinatorial derivations --- temp3.py | 387 +++++++++++++++++++++++++++++++++++-------------------- 1 file changed, 245 insertions(+), 142 deletions(-) diff --git a/temp3.py b/temp3.py index b83ac65..5fb33b6 100644 --- a/temp3.py +++ b/temp3.py @@ -6,185 +6,288 @@ direct = os.path.split(__file__)[0] file_path_in = os.path.join(direct, 'SBOL3_simple_library4.nt') +# Purpose: To convert combinatorial derivation objects to component objects if necessary +# Input: SBOL3 file reference +# Output: SBOL3 file in new path, updated document object + + +def convCombDeriv(file_path_in): + # read in the document as an rdf graph + g = rdflib.Graph() + result = g.parse(file_path_in) + + # Creating the Sbol document to be changed from the conversion + doc = sbol3.Document() + doc.read(file_path_in) + + # Get all objects from the graph + dictionaryObj = doc._parse_objects(g) + + # Find all combinatorial derivation objects (identity: object) + tempcombdev = {} + combdev = {} + + for item in dictionaryObj: + if type(dictionaryObj[item]) == sbol3.CombinatorialDerivation: + tempcombdev[item] = dictionaryObj[item] + + # 1. Check to see if the object has an inserted construct + for item in dictionaryObj: + ins = item + "_ins" + if ins in dictionaryObj: + combdev[item] = dictionaryObj[item] + tempcombdev.pop(item) + tempcombdev.pop(ins) + + + # 2. Go through the remaining combinatorial derivation objects and check each variable feature to see if it has more than one variant + for item in tempcombdev: + temp = doc.find(item) + for variable_feature in temp.variable_features: + if len(variable_feature.variants) > 1: + combdev[item] = dictionaryObj[item] + break + + for item in combdev: + if item in tempcombdev: + tempcombdev.pop(item) + + # 3. Go through the remaining objects and check to see if their variable feature contains a combinatorial derivation variant + for item in tempcombdev: + temp = doc.find(item) + for variable_feature in temp.variable_features: + for variant in variable_feature.variants: + if variant in combdev: + combdev[item] = tempcombdev[item] + break + + + + # 4. For every item in tempcombdev (Not a combinatorial derivation), convert it to a component + + newComponents = {} + for item in tempcombdev: + # From combinatorial derivation: name, displayId, namespace + obj = doc.find(item) + + newComp = sbol3.Component(identity=obj.identity, types=sbol3.component.SBOL_COMPONENT) + newComp.name = obj.name + newComp.displayId = obj.display_id + newComp.namespace = obj.namespace + + # From template: features, constraints, type + template = doc.find(f'{obj.identity}_template') + + for feature in template.features: + if type(feature) != sbol3.LocalSubComponent: + subComp = sbol3.SubComponent(instance_of=feature.instance_of) + newComp.features.append(subComp) + + for constraint in template.constraints: + newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) + newConstraint.derived_from = constraint.derived_from + newComp.constraints.append(newConstraint) + + newComponents[item] = newComp + + # 5. Delete the original combinatorial derivation object and template + for item in tempcombdev: + obj_doc = doc.find(item) + doc.remove_object(obj_doc) + + template = doc.find(f'{item}_template') + doc.remove_object(template) + + # 6. Add the new component object to the document + for item in newComponents: + doc.add(newComponents[item]) + + # 7. Update the uri references + oldToNew = {} + + for item in tempcombdev: + oldToNew[tempcombdev[item].identity] = newComponents[item].identity + + h.update_uri_refs(doc, oldToNew) + + return doc + + + +doc = convCombDeriv(file_path_in) + +file_path_out = "SampleTemp3Output.nt" +doc.write(file_path_out) -# read in the document as an rdf graph -g = rdflib.Graph() -result = g.parse(file_path_in) -# Creating the Sbol document to be changed from the conversion -doc = sbol3.Document() -doc.read(file_path_in) # collect all the objects which are combinatorial derivations and their featured components -knows_query = """ -SELECT - ?subject - ?object -WHERE { - ?subject ?template . - ?template ?feature . - ?feature ?object . -}""" -qres = g.query(knows_query) - -# create a tree structure -children = set() # all objects that are referenced by another object -parent_dict = {} # all objects that reference another object and the objects they reference as a set -for row in qres: - s = str(row.subject) - o = str(row.object) - children.add(o) - if s in parent_dict: - parent_dict[s].add(o) - else: - parent_dict[s] = {o} - -parent_dict_copy = parent_dict - -print(parent_dict) - -# objects that are only children are objects that are not also parents -child_only = children - set(parent_dict).intersection(children) - -# Loop through children create a dictionary for the object and the type of the object to look at in while loop -# Keep track of the old uris and new uris in case they change - function already exists to change in document - -old_uris = [] # Correlates index to index with each of the new uris -new_uris = [] - -# process the children only objects and update the 'tree' -while len(parent_dict) > 0: - # do something to deal with child_only - # for this you probably need to read in the sbol document at the top and perform actions using pysbol - - # Import SBOL document as the SBOL document type - # Go through each of the children and determine if it is a combinatorial derivation - may have to save a copy of the parent dictionary - # Go through each of the uris in the child object and look to see if those uris in parent dictionary are combinatorial derivations - going to need pysbol - # Take it out as a string and check what the type is in the object in the sbol document - # May have to write in another file for now - # Object stays combinatorial derivation if one of its subcomponents is a combinatorial derivation - # Else: Becomes a component - # For collection: If has a variable feature and nothing else + +# knows_query = """ +# SELECT +# ?subject +# ?object +# WHERE { +# ?subject ?template . +# ?template ?feature . +# ?feature ?object . +# }""" +# qres = g.query(knows_query) + +# # create a tree structure +# children = set() # all objects that are referenced by another object +# parent_dict = {} # all objects that reference another object and the objects they reference as a set +# for row in qres: +# s = str(row.subject) +# o = str(row.object) +# children.add(o) +# if s in parent_dict: +# parent_dict[s].add(o) +# else: +# parent_dict[s] = {o} + +# parent_dict_copy = parent_dict + +# # objects that are only children are objects that are not also parents +# child_only = children - set(parent_dict).intersection(children) + +# # Loop through children create a dictionary for the object and the type of the object to look at in while loop +# # Keep track of the old uris and new uris in case they change - function already exists to change in document + +# old_uris = [] # Correlates index to index with each of the new uris +# new_uris = [] + +# # process the children only objects and update the 'tree' +# while len(parent_dict) > 0: +# # do something to deal with child_only +# # for this you probably need to read in the sbol document at the top and perform actions using pysbol + +# # Import SBOL document as the SBOL document type +# # Go through each of the children and determine if it is a combinatorial derivation - may have to save a copy of the parent dictionary +# # Go through each of the uris in the child object and look to see if those uris in parent dictionary are combinatorial derivations - going to need pysbol +# # Take it out as a string and check what the type is in the object in the sbol document +# # May have to write in another file for now +# # Object stays combinatorial derivation if one of its subcomponents is a combinatorial derivation +# # Else: Becomes a component +# # For collection: If has a variable feature and nothing else - # loop over parents +# # loop over parents - for parent in parent_dict: - if parent_dict[parent].issubset(child_only): # If the parent's children are a subset of childOnly - print(parent) # Print the name of the parent object - child_types = [] # Create a list of all of the types of child objects - for child in parent_dict[parent]: - children_of_parent = set() - obj = doc.find(child) - children_of_parent.add(type(obj)) +# for parent in parent_dict: +# if parent_dict[parent].issubset(child_only): # If the parent's children are a subset of childOnly +# # print(parent) # Print the name of the parent object +# child_types = [] # Create a list of all of the types of child objects +# for child in parent_dict[parent]: +# children_of_parent = set() +# obj = doc.find(child) +# children_of_parent.add(type(obj)) - # if len(child_types) == len(parent_dict[parent]): # Check to see that it's the end of the loop - # Trying to see if it can be a collection object - if len(children_of_parent) == 0: # Checking to see if there aren't any children and only contains a variable feature - parentObj = doc.find(parent) # In template not in the parent - template = doc.find(f'{parent}_template') # Get the template object from the parent +# # if len(child_types) == len(parent_dict[parent]): # Check to see that it's the end of the loop +# # Trying to see if it can be a collection object +# if len(children_of_parent) == 0: # Checking to see if there aren't any children and only contains a variable feature +# parentObj = doc.find(parent) # In template not in the parent +# template = doc.find(f'{parent}_template') # Get the template object from the parent - if len(template.features) == 1 and type(template.features[0]) == sbol3.VariableFeature: # If it only has 1 variable feature and nothing else - collection object - col = sbol3.Collection() - for variant in template.features[0]: # Add all variants of the variable feature to the collection - col.members.append(variant) +# if len(template.features) == 1 and type(template.features[0]) == sbol3.VariableFeature: # If it only has 1 variable feature and nothing else - collection object +# col = sbol3.Collection() +# for variant in template.features[0]: # Add all variants of the variable feature to the collection +# col.members.append(variant) - # Delete original comb der + template after adding in uris to appropriate arrays - old_uris.append(parentObj.identity) - new_uris.append(col.identity) +# # Delete original comb der + template after adding in uris to appropriate arrays +# old_uris.append(parentObj.identity) +# new_uris.append(col.identity) - doc.remove_object(parentObj) - doc.remove_object(template) +# doc.remove_object(parentObj) +# doc.remove_object(template) - if sbol3.CombinatorialDerivation not in children_of_parent: # If there is no child that is a combinatorial derivation - # Delete the template that's currently in the document - # Create a new component object and carry over all of the features from the combinatorial derivation (including exact name) - # Name, (references to) subcomponents, (references to) constraints, name, displayId, role, type - # Delete the previous combinatorial derivation object - # Put the new component object into the document +# if sbol3.CombinatorialDerivation not in children_of_parent: # If there is no child that is a combinatorial derivation +# # Delete the template that's currently in the document +# # Create a new component object and carry over all of the features from the combinatorial derivation (including exact name) +# # Name, (references to) subcomponents, (references to) constraints, name, displayId, role, type +# # Delete the previous combinatorial derivation object +# # Put the new component object into the document - parentObj = doc.find(parent) # Get the object that the parent is supposed to be +# parentObj = doc.find(parent) # Get the object that the parent is supposed to be - template = doc.find(f'{parent}_template') # Get the template object from the parent +# template = doc.find(f'{parent}_template') # Get the template object from the parent - # print(parentObj._properties) - old_uris.append(parentObj.identity) # Add the uri of the old parent object to the list +# # print(parentObj._properties) +# old_uris.append(parentObj.identity) # Add the uri of the old parent object to the list - componentObj = sbol3.Component(identity=parentObj.identity, types=parentObj.type_uri) # Create a new component object +# componentObj = sbol3.Component(identity=parentObj.identity, types=parentObj.type_uri) # Create a new component object - new_uris.append(componentObj.identity) # Add its URI to the list of new uris +# new_uris.append(componentObj.identity) # Add its URI to the list of new uris - componentObj.name = parentObj.name # Set the name +# componentObj.name = parentObj.name # Set the name - print(parentObj._properties) +# # print(parentObj._properties) - for feature in template.features: - if type(feature) != sbol3.LocalSubComponent: - subComp = sbol3.SubComponent(instance_of=feature.instance_of) - componentObj.features.append(subComp) # +# for feature in template.features: +# if type(feature) != sbol3.LocalSubComponent: +# subComp = sbol3.SubComponent(instance_of=feature.instance_of) +# componentObj.features.append(subComp) # - for role in template.roles: - componentObj.roles.append(role) +# for role in template.roles: +# componentObj.roles.append(role) - # Still need to find out how to attribute subcomponents, constraints (Both subcomponents and constraints and roles in template) - for prop in parentObj._properties: - for subProp in parentObj._properties[prop]: - if prop == "http://sbols.org/v3#template": - continue +# # Still need to find out how to attribute subcomponents, constraints (Both subcomponents and constraints and roles in template) +# for prop in parentObj._properties: +# for subProp in parentObj._properties[prop]: +# if prop == "http://sbols.org/v3#template": +# continue - if type(subProp) == rdflib.term.URIRef: - setattr(componentObj, prop, - sbol3.URIProperty(componentObj, - f'{prop}', - '0', '*', initial_value=[subProp])) - else: - setattr(componentObj, prop, - sbol3.TextProperty(componentObj, - prop, - '0', '*', initial_value=str(subProp))) +# if type(subProp) == rdflib.term.URIRef: +# setattr(componentObj, prop, +# sbol3.URIProperty(componentObj, +# f'{prop}', +# '0', '*', initial_value=[subProp])) +# else: +# setattr(componentObj, prop, +# sbol3.TextProperty(componentObj, +# prop, +# '0', '*', initial_value=str(subProp))) - for constraint in template.constraints: - newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) - newConstraint.derived_from = constraint.derived_from - componentObj.constraints.append(newConstraint) - # Add all to component - # Eventually should be more filtered +# for constraint in template.constraints: +# newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) +# newConstraint.derived_from = constraint.derived_from +# componentObj.constraints.append(newConstraint) +# # Add all to component +# # Eventually should be more filtered - componentObj.types[0] = sbol3.component.SBOL_COMPONENT +# componentObj.types[0] = sbol3.component.SBOL_COMPONENT - print(componentObj._properties) +# # print(componentObj._properties) - doc.remove_object(parentObj) # Remove the old object - doc.remove_object(template) # Remove the old template +# doc.remove_object(parentObj) # Remove the old object +# doc.remove_object(template) # Remove the old template - doc.add(componentObj) # Add the new object +# doc.add(componentObj) # Add the new object - # print(child, parent_dict_copy[child]) +# # print(child, parent_dict_copy[child]) - # remove child only children from dictionary - new_parent_dict = {} +# # remove child only children from dictionary +# new_parent_dict = {} - for key in parent_dict: - current_children = parent_dict[key] - remaining_children = current_children - current_children.intersection(child_only) - if len(remaining_children) > 0: - new_parent_dict[key] = remaining_children - parent_dict = new_parent_dict +# for key in parent_dict: +# current_children = parent_dict[key] +# remaining_children = current_children - current_children.intersection(child_only) +# if len(remaining_children) > 0: +# new_parent_dict[key] = remaining_children +# parent_dict = new_parent_dict - # update child_only list - child_only = children - set(parent_dict).intersection(children) +# # update child_only list +# child_only = children - set(parent_dict).intersection(children) -# Update uris on document using the old uri and new uri comparison -oldToNew = {} -for i in range(len(old_uris)): - oldToNew[old_uris[i]] = new_uris[i] +# # Update uris on document using the old uri and new uri comparison +# oldToNew = {} +# for i in range(len(old_uris)): +# oldToNew[old_uris[i]] = new_uris[i] -h.update_uri_refs(doc, oldToNew) +# h.update_uri_refs(doc, oldToNew) # return updated sbol document -file_path_out = "SampleTemp3Output.nt" -doc.write(file_path_out) \ No newline at end of file From d2b3895759b871371340c4e13873f97db01920d6 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 20 Jun 2023 11:47:25 -0600 Subject: [PATCH 08/17] Added correct namespace for sequences and made the string literal of elements uppercase --- excelutils/excel_sbol_utils/library3.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/excelutils/excel_sbol_utils/library3.py b/excelutils/excel_sbol_utils/library3.py index 7ef244b..4db8a5e 100644 --- a/excelutils/excel_sbol_utils/library3.py +++ b/excelutils/excel_sbol_utils/library3.py @@ -218,11 +218,11 @@ def sequence(rowobj): # removes spaces, enters, and makes all lower case val = "".join(val.split()) - val = val.replace(u"\ufeff", "").lower() + val = val.replace(u"\ufeff", "").upper() # create sequence object - sequence = sbol3.Sequence(f"{rowobj.obj.displayId}_sequence", - elements=val) + sequence = sbol3.Sequence(f"{rowobj.obj.namespace}/{rowobj.obj.display_id}_sequence", + elements=val, encoding=sbol3.IUPAC_DNA_ENCODING, namespace=rowobj.obj.namespace) if rowobj.obj.name is not None: sequence.name = f"{rowobj.obj.name} Sequence" From 100a75492b64ecf6f50e3fc4b7f4ca357c421cb9 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 31 Aug 2023 10:22:49 -0600 Subject: [PATCH 09/17] Addition of output from temp3.py including updates in temp3.py. --- SampleTemp3Output.nt | 677 +++++++++++++++++++++++++++++++++++++++++++ temp3.py | 426 ++++++++++++++++----------- 2 files changed, 929 insertions(+), 174 deletions(-) create mode 100644 SampleTemp3Output.nt diff --git a/SampleTemp3Output.nt b/SampleTemp3Output.nt new file mode 100644 index 0000000..c19ceb2 --- /dev/null +++ b/SampleTemp3Output.nt @@ -0,0 +1,677 @@ + "Green FP (off patent)\nGFPmut3" . + "E0040" . + . + . + "GFPmut3" . + . + . + . + "E0040_sequence" . + "ATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAA" . + . + . + . + "Red FP (off patent)\nmRFP1" . + "E1010" . + . + . + "mRFP1" . + . + . + . + "E1010_sequence" . + "ATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGC" . + . + . + . + "positive control 2018 interlab" . + "I20270" . + . + . + "I20270" . + . + . + . + "I20270_sequence" . + "TTGATGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Red FP (off patent)\nmCherry" . + "J06504" . + . + . + "J06504" . + . + . + . + "J06504_sequence" . + "ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCTTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG" . + . + . + . + "5.5 SLM (high, but variable)" . + "J23101" . + . + . + "J23101" . + . + . + . + "J23101_sequence" . + "TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC" . + . + . + . + "TBD SLM (expect 4.5)" . + "J23105" . + . + . + "J23105" . + . + . + . + "J23105_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGC" . + . + . + . + "J23106 2018 interlab" . + "J364001" . + . + . + "J364001" . + . + . + . + "J364001_sequence" . + "TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23117 2018 interlab" . + "J364002" . + . + . + "J364002" . + . + . + . + "J364002_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23100 2018 interlab" . + "J364007" . + . + . + "J364007" . + . + . + . + "J364007_sequence" . + "TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23116 2018 interlab" . + "J364009" . + . + . + "J364009" . + . + . + . + "J364009_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Blue FP\nTagBFP2" . + "K592100" . + . + . + "K592100" . + . + . + . + "K592100_sequence" . + "ATGAGCGAACTGATCAAAGAGAACATGCACATGAAGCTGTACATGGAAGGCACCGTTGACAACCACCACTTTAAGTGCACGTCTGAGGGTGAGGGTAAGCCGTACGAAGGCACCCAAACCATGCGTATCAAAGTTGTGGAGGGCGGTCCACTGCCGTTCGCTTTTGACATTCTGGCGACCAGCTTCCTGTACGGTTCCAAAACGTTCATTAACCATACTCAGGGCATTCCGGATTTCTTCAAACAGAGCTTTCCGGAAGGTTTCACCTGGGAGCGTGTCACCACGTATGAAGATGGTGGTGTGTTGACCGCCACCCAAGATACCTCCCTGCAAGATGGCTGTCTGATCTATAACGTGAAAATTCGTGGCGTCAACTTTACGAGCAATGGTCCGGTGATGCAGAAGAAAACCCTGGGTTGGGAGGCGTTTACGGAAACCCTGTATCCGGCCGATGGTGGCCTGGAGGGCCGTAACGACATGGCACTGAAGCTGGTTGGTGGCAGCCATTTGATCGCAAATATCAAGACGACGTACCGCAGCAAGAAACCGGCGAAAAATCTGAAGATGCCGGGTGTTTACTATGTCGACTACCGTCTGGAACGCATTAAAGAAGCGAATAATGAGACTTACGTGGAGCAGCACGAGGTTGCAGTCGCGCGCTATTGCGACTTGCCTAGCAAGCTGGGTCATAAACTGAAT" . + . + . + . + "negative control 2018 interlab" . + "R0040" . + . + . + "R0040" . + . + . + . + "R0040_sequence" . + "TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCAC" . + . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + . + . + . + "All_FPs" . + . + . + "All FPs" . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "All_FPs_template" . + . + . + . + . + "B0015" . + . + . + "B0015" . + . + . + . + "B0015_sequence" . + "CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Moderate (?)" . + "B0032" . + . + . + "B0032" . + . + . + . + "B0032_sequence" . + "TCACACAGGAAAG" . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "SubComponent3" . + . + . + . + "5' UTR including B0032 RBS and BB scars (long and short)" . + "BB_B0032_BB" . + . + . + . + . + . + . + "BB-B0032-BB" . + . + . + . + "Designs for round 1 if iGEM 2021 calibration experimentation" . + "BasicParts" . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + "Fluorescence calibration basic parts" . + . + "BioBricks scar including ag (not before CDS)" . + "BioBricks_scar_long" . + . + . + "BioBricks_scar_long" . + . + . + . + "BioBricks_scar_long_sequence" . + "TACTAGAG" . + . + . + . + "BioBricks scar (short one, w/o ag, before CDS)" . + "BioBricks_scar_short" . + . + . + "BioBricks_scar_short" . + . + . + . + "BioBricks_scar_short_sequence" . + "TACTAG" . + . + . + . + "Designs for round 1 if iGEM 2021 calibration experimentation" . + "CompositeParts" . + . + . + . + . + . + . + . + "Fluorescence calibration devices" . + . + "Cyan FP; codon optimized using IDT tool \nECFP" . + "ECFP" . + . + . + "ECFP" . + . + . + . + . + "ECFP_sequence" . + "ATGGTTAGTAAAGGGGAAGAGTTGTTTACGGGCGTTGTTCCAATCTTGGTGGAGTTGGACGGGGACGTTAATGGGCACAAATTTTCGGTCTCTGGGGAAGGCGAGGGGGACGCCACGTATGGTAAGCTGACCTTGAAGTTCATTTGCACTACTGGGAAATTGCCGGTTCCCTGGCCTACGCTTGTTACCACGTTGACCTGGGGCGTACAATGTTTCTCGCGCTATCCTGACCACATGAAACAACATGATTTCTTTAAGTCAGCTATGCCTGAGGGCTACGTGCAGGAGCGCACTATCTTCTTCAAGGATGACGGGAATTACAAAACCCGTGCGGAGGTAAAGTTCGAGGGTGACACGCTTGTCAACCGCATCGAGTTGAAGGGCATTGATTTTAAGGAGGATGGCAACATTTTAGGACATAAATTAGAGTATAACTATATTTCGCATAATGTCTACATTACTGCTGACAAACAAAAGAACGGCATCAAGGCTAATTTCAAAATTCGCCACAACATCGAGGATGGATCGGTTCAGTTAGCAGATCATTACCAGCAAAACACGCCCATCGGTGACGGACCAGTTCTGTTACCTGACAACCACTATCTTTCTACACAGAGTGCTCTTAGCAAGGACCCTAACGAAAAACGTGACCACATGGTCCTGCTGGAGTTTGTGACAGCCGCAGGTATTACATTGGGGATGGACGAATTGTATAAG" . + . + . + . + . + "VariableFeature1" . + . + . + . + "FPs_small" . + . + . + "FPs small" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + "VariableFeature2" . + . + . + . + "FPs_small_ins" . + . + . + . + "FPs small insert" . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "Constraint3" . + . + . + . + . + "Constraint4" . + . + . + . + . + "Constraint5" . + . + . + . + . + "LocalSubComponent1" . + "Part 2" . + . + . + . + "LocalSubComponent2" . + "Part 4" . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "SubComponent3" . + . + . + . + "SubComponent4" . + . + . + . + "FPs_small_ins_template" . + . + . + . + . + . + . + . + . + . + . + . + . + "FPs_small_ins_template" . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "FPs_small_template" . + . + . + . + . + . + . + . + "FinalProducts" . + . + . + . + "Final Products" . + . + "" . + "LinearDNAProducts" . + . + . + . + "Linear DNA Products" . + . + . + "VariableFeature1" . + . + . + . + . + "Round_1_order" . + . + . + "Round 1 order" . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "Round_1_order_template" . + . + . + . + . + "2 stop codons" . + "STOP" . + . + . + "STOP" . + . + . + . + "STOP_sequence" . + "TAATAA" . + . + . + . + "UNSX" . + . + . + "UNSX" . + . + . + . + "Constraint1" . + . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "UNSX_UP" . + . + . + . + . + "UNSX-UP" . + . + . + . + "UNSX_sequence" . + "CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC" . + . + . + . + "consensus without runs of As and Ts -59 to -36" . + "UP_element_consensus" . + . + . + "UP element consensus" . + . + . + . + "UP_element_consensus_sequence" . + "TGAAATATATTTTCGAAAAGCCGC" . + . + . + . + . + "VariableFeature1" . + . + . + . + "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . + "_2018_Interlab" . + . + . + "2018 Interlab" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + "_2018_Interlab_ins" . + . + . + "2018 Interlab insert" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "_2018_Interlab_ins_template" . + . + . + "_2018_Interlab_ins_template" . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "_2018_Interlab_template" . + . + . + . + . + . + . + . + "Blue FP codon optimized" . + "mTagBFP2" . + . + . + "mTagBFP2" . + . + . + . + "mTagBFP2_sequence" . + "ATGGTATCGAAGGGTGAAGAGTTGATTAAGGAAAACATGCACATGAAGTTATACATGGAAGGGACCGTGGATAATCACCATTTCAAATGCACATCTGAAGGAGAGGGAAAACCTTACGAAGGGACCCAGACAATGCGTATCAAAGTGGTCGAGGGTGGCCCTTTACCATTCGCGTTCGACATCCTGGCGACCTCCTTTTTATATGGGAGCAAAACATTCATTAACCACACCCAGGGAATCCCGGATTTCTTCAAGCAGTCTTTCCCTGAAGGGTTTACGTGGGAGCGTGTGACAACTTATGAGGATGGCGGGGTTTTGACGGCTACGCAGGATACTTCGTTACAAGACGGTTGCTTAATTTACAATGTAAAGATTCGTGGAGTAAATTTTACATCAAACGGTCCGGTCATGCAAAAGAAAACCCTTGGTTGGGAGGCGTTTACCGAGACATTGTATCCTGCCGACGGGGGATTAGAGGGTCGCAATGATATGGCACTGAAACTTGTGGGGGGGTCTCATTTAATTGCAAACGCTAAGACAACTTACCGCTCCAAGAAGCCCGCTAAGAACCTGAAAATGCCTGGGGTCTACTATGTTGACTATCGCCTTGAGCGTATCAAGGAGGCGAACAACGAGACTTACGTCGAGCAGCATGAGGTAGCGGTTGCCCGTTACTGTGACCTGCCATCTAAGTTAGGACATAAGTTGAAT" . + . + . + . + "Cyan FP (free); codon optimized using IDT tool \nmeffCFP" . + "meffCFP" . + . + . + "meffCFP" . + . + . + . + . + "meffCFP_sequence" . + "ATGGCGCTTTCAAAGCAGAGTTTACCGAGTGACATGAAGTTGATCTACCACATGGACGGGAACGTGAATGGTCACTCTTTCGTCATTAAGGGTGAAGGCGAGGGAAAACCCTATGAAGGAACACATACTATCAAGCTGCAAGTTGTTGAGGGCTCACCGTTACCTTTCAGCGCCGACATTCTGTCCACAGTTTTTCAATATGGGAACCGCTGTTTCACTAAGTATCCGCCTAATATCGTCGATTATTTTAAGAATAGCTGTTCTGGTGGGGGCTACAAGTTTGGACGCAGCTTTCTTTACGAAGACGGTGCGGTCTGTACGGCGTCAGGCGATATTACTCTGTCCGCAGACAAAAAGAGCTTTGAACACAAATCGAAATTCTTAGGCGTGAACTTCCCGGCTGACGGTCCGGTGATGAAGAAAGAGACCACCAACTGGGAACCATCGTGCGAAAAAATGACTCCGAATGGCATGACACTGATCGGTGACGTTACGGGTTTTTTGTTGAAGGAGGACGGAAAGCGTTACAAATGTCAATTTCATACCTTCCATGACGCTAAAGATAAGTCCAAAAAGATGCCGATGCCCGACTTCCACTTTGTCCAGCATAAAATTGAGCGCAAGGACCTTCCTGGAAGTATGCAAACATGGCGTCTTACCGAGCACGCGGCAGCATGTAAAACTTGTTTTACGGAG" . + . + . + . + "Blue FP (free); codon optimized using IDT tool \nmmilCFP" . + "mmilCFP" . + . + . + "mmilCFP" . + . + . + . + . + "mmilCFP_sequence" . + "ATGGCTCTGCCTAAACAAATGAAACTGACTTATCACATGGAGGGTACTGTGAACGGTCATTTTTTTATCATCAAGGGCGAAGGAGGGGGAGAGCCGTATGAGGGTACTCATACGATCAAGTTACAGGTGGTAGAGGGAAGCCCGTTGCCCTTTTCACCGGATATTCTTTCCACCGTCTTCCAATACGGCAACCGCTGCTTCACTAAATACCCTCCCAACATCGTTGATTACTTCAAAAACTCCTGCTCGGGCGGCGGTTATACTTTTGGACGTAGCTTCCTTTATGAGGACGGAGCTGTCTGTACTGCTTCCGGTGACATTACGCTGAGCAGCGACAAATCATCCTTTGAGCACAAATCTAAATTTCTGGGAGTAAACTTTCCTGCCGATGGCCCGGTGATGAAAAAAGAAACCACTAATTGGGAGCCTTCATGTGAGAAAATGACACCCAACGGGATGACCTTAATCGGTGATGTGACGGAGTTCCTGTTGAAGAAGGACGGTAAGCGTTACAAGTGCCAATTCCACACATTCCACGACGCGAAGGAGAAAAGCCGCAATATGCCGATGCCCGATTTCCATTTCGTTCAGCACGAGATTGAACGTAAAGATTTGCCAGGACCAATGCAAACATGGCAGTTGACCGAGCACGCAGCAGCCTGTAAGAACTGTTTCACAGAG" . + . + . + . + "pMB1 replicon, 100-300 copy" . + "pSB1C3" . + . + "pSB1C3" . + . + . + . + "LmrA" . + . + "LmrA" . + . + . + . + . + "Cyan FP; codon optimized using IDT tool \nmCerulean3" . + "ATP07149_1" . + . + . + "mCerulean3" . + . + . + . + . + "ATP07149_1_sequence" . + "ATGGTGTCGAAAGGTGAAGAACTTTTTACAGGTGTGGTCCCCATTTTAGTCGAATTAGATGGGGATGTGAATGGTCACAAATTCTCGGTGTCTGGGGAGGGTGAAGGTGACGCGACCTACGGGAAGTTGACTCTGAAATTCATTTGCACTACGGGGAAGTTACCAGTGCCCTGGCCCACTCTGGTTACAACACTGTCATGGGGCGTCCAATGTTTTGCTCGTTATCCTGACCACATGAAACAGCATGACTTCTTCAAGAGCGCAATGCCGGAAGGCTATGTCCAAGAGCGTACTATCTTTTTCAAAGACGATGGGAACTACAAAACACGCGCAGAAGTGAAGTTTGAGGGAGATACCCTGGTTAATCGCATTGAATTGAAAGGTATCGACTTCAAGGAGGATGGTAATATCCTGGGGCACAAATTGGAGTACAACGCTATCCACGGCAATGTTTACATTACGGCAGACAAACAGAAAAACGGAATTAAGGCCAATTTCGGACTTAACTGCAACATCGAAGATGGGTCGGTACAACTGGCAGACCACTATCAGCAAAATACGCCTATTGGTGACGGTCCTGTCCTGTTACCGGACAATCATTATCTTTCCACTCAGTCGAAACTGAGTAAAGATCCGAACGAGAAGCGCGATCACATGGTATTATTAGAGTTCGTCACAGCCGCAGGTATTACGTTAGGGATGGATGAGCTTTACAAG" . + . + . + . diff --git a/temp3.py b/temp3.py index 5fb33b6..0b5ad59 100644 --- a/temp3.py +++ b/temp3.py @@ -6,11 +6,249 @@ direct = os.path.split(__file__)[0] file_path_in = os.path.join(direct, 'SBOL3_simple_library4.nt') +# Purpose: After converting the combinatorial derivations, the references to the objects in other parts must be updated +# Input: SBOL3 Document object, SBOL3 Dictionary of combinatorial derivations after conversion +# Output: Updated SBOL3 Document object + +def updateVariableFeatures(doc, combdev): + # 1: Loop through every item in the combdev dictionary + for item in combdev: + # 2 Cases: Has a backbone and therefore variable features located in insert, or located inside the original object + convVar = [] + ins = True # If the object has an insert + edited = False # If the variable features are edited + + insert = item + "_ins" + obj = doc.find(insert) + + if obj == None: + ins = False + obj = doc.find(item) + + if ins: + template = doc.find(f'{insert}_template') + else: + template = doc.find(f'{item}_template') + + + # 2: Go through every variable feature + for variable_feature in list(obj.variable_features): + + if len(variable_feature.variants) > 1: + continue # Leave as a variable feature + else: + variant = variable_feature.variants[0] + if str(variant) not in combdev: + edited = True + + # 1. Using the variable of the variable feature, find the local subcomponent in the template and retrieve the part number + + localsub = doc.find(variable_feature.variable) + + tempsub = sbol3.SubComponent(instance_of=variant, name=localsub.name, orientation=sbol3.SBOL_INLINE) + + # Add the subcomponent to the template + + template.features.append(tempsub) + + # Change the constriant to the subcomponent + + for constraint in template.constraints: + if constraint.subject == variable_feature.variable: + constraint.subject = tempsub.identity + if constraint.object == variable_feature.variable: + constraint.object = tempsub.identity + + # Remove the localsubcomponent and the variable feature + + template.features.remove(localsub) + obj.variable_features.remove(variable_feature) + + + if edited: + + + localsubs = [None] * 100 # Used for relabeling + subs = [None] * 100 # subcomponents + variableFeatures = {} + copiedVariableFeatures = [None] * 100 + partsToFeatures = {} + + # Go through remaining variable features and create a dictionary with key = list of variants and value = part # of variants + # From this, delete the variable features and add their copies back to their template + # Then delete the original variable, and add the new variable based on the variants + + for variable_feature in list(obj.variable_features): + variants = [] + for variant in variable_feature.variants: + variants.append(str(variant)) + variants = tuple(variants) + + # Now find the part number from the variable + + localsub = doc.find(variable_feature.variable) + + number = int(localsub.name.split(" ")[1]) + variableFeatures[variants] = number + + # Copy the variable features and add them to copiedVariableFeatures + + copyFeature = sbol3.VariableFeature(variants=variable_feature.variants, name=variable_feature.name, variable=variable_feature.variable, cardinality=variable_feature.cardinality) + copiedVariableFeatures[number] = copyFeature + + # Remove the variable feature from the object + + obj.variable_features.remove(variable_feature) + + # Update the references + + + # Add the copied variable features back to the template + + for var in copiedVariableFeatures: + if var != None: + obj.variable_features.append(var) + + + for feature in list(template.features): + if type(feature) == sbol3.LocalSubComponent: + copyFeature = sbol3.LocalSubComponent(types=feature.type_uri, name=feature.name, orientation=feature.orientation) + localsubs[int(feature.name.split(" ")[1])] = copyFeature + template.features.remove(feature) + elif type(feature) == sbol3.SubComponent: + copyFeature = sbol3.SubComponent(instance_of=feature.instance_of, name=feature.name, orientation=feature.orientation) + subs[int(feature.name.split(" ")[1])] = copyFeature + template.features.remove(feature) + + + for item in localsubs: + if item != None: + template.features.append(item) + + for item in subs: + if item != None: + template.features.append(item) + + # Fix variable features to reference the correct localsubcomponent + + for variable_feature in list(obj.variable_features): + variants = [] + for variant in variable_feature.variants: + variants.append(str(variant)) + + variants = tuple(variants) + + number = variableFeatures[variants] + + for feature in list(template.features): + if type(feature) == sbol3.LocalSubComponent: + if int(feature.name.split(" ")[1]) == number: + variable_feature.variable = feature.identity + break + + # Fix all of the constraints + + # Go through every subcomponent and localsubcomponent and do key: part #, value = displayID + + for feature in list(template.features): + if type(feature) == sbol3.LocalSubComponent: + partsToFeatures[int(feature.name.split(" ")[1])] = feature.identity + elif type(feature) == sbol3.SubComponent: + partsToFeatures[int(feature.name.split(" ")[1])] = feature.identity + + # Delete all constraints + + template.constraints.clear() + + # Add constraints one by one with part 1 being the subject and part 2 being the object until the end of the dictionary + + part = 1 + while part < len(partsToFeatures): + constraint = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, subject=partsToFeatures[part], object=partsToFeatures[part + 1]) + template.constraints.append(constraint) + part += 1 + + # Clear the part # from the subcomponents + + for feature in list(template.features): + if type(feature) == sbol3.SubComponent: + del feature._properties[sbol3.SBOL_NAME] + + + + + return None + + +# Purpose: Update the uri references in the LinearDNAProducts to the _ins versions of the parts +# Input: SBOL3 document object +# Output: SBOL3 document object with updated references + +def updateLinearDNAProducts(doc): + # Go through every object in the doc + # If it's a collection, then check if it's the LinearDNAProducts collection + # For every member, get the identity, then replace it with the _ins version of the part + + for obj in doc.objects: + if type(obj) == sbol3.Collection: + if obj.display_id == 'LinearDNAProducts': + tempList = [] + for member in obj.members: + mem = doc.find(member) + memberid = mem.identity + insConstruct = doc.find(memberid + "_ins") + displah_id = insConstruct.identity + tempList.append(displah_id) + obj.members = tempList + + return doc + + +# Purpose: Update the collection names for basic parts and composite parts +# Input: SBOL3 document object +# Output: SBOL3 document object with updated collection names + +def updateCollectionNames(doc): + # Change the basic parts collection name to basicparts (if it exists) + + # Lists to go through to either add or take off of the document once completed + removeList = [] + addList = [] + + for obj in doc.objects: + if type(obj) == sbol3.Collection: + + # Set the namespace for sbol3 so that the collections contain the correct namespace and are named correctly + sbol3.set_namespace(obj.namespace) + + if obj.display_id == 'Basic_Parts': + newCollection = sbol3.Collection(identity='BasicParts', name=obj.name, members=obj.members, description=obj.description) + + removeList.append(obj) + addList.append(newCollection) + + elif obj.display_id == 'Composite_Parts': + newCollection = sbol3.Collection(identity='CompositeParts', name=obj.name, members=obj.members, description=obj.description) + + removeList.append(obj) + addList.append(newCollection) + + for item in removeList: + doc.remove_object(item) + + for item in addList: + doc.add(item) + + return doc + + + + + # Purpose: To convert combinatorial derivation objects to component objects if necessary # Input: SBOL3 file reference # Output: SBOL3 file in new path, updated document object - def convCombDeriv(file_path_in): # read in the document as an rdf graph g = rdflib.Graph() @@ -74,17 +312,20 @@ def convCombDeriv(file_path_in): newComp.name = obj.name newComp.displayId = obj.display_id newComp.namespace = obj.namespace + newComp.roles = [sbol3.SO_ENGINEERED_REGION] + newComp.types = [sbol3.SBO_DNA] + newComp.description = obj.description # From template: features, constraints, type template = doc.find(f'{obj.identity}_template') for feature in template.features: if type(feature) != sbol3.LocalSubComponent: - subComp = sbol3.SubComponent(instance_of=feature.instance_of) + subComp = sbol3.SubComponent(instance_of=feature.instance_of, orientation=feature.orientation) newComp.features.append(subComp) for constraint in template.constraints: - newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) + newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject.replace('_template', ''), constraint.object.replace('_template', '')) newConstraint.derived_from = constraint.derived_from newComp.constraints.append(newConstraint) @@ -102,7 +343,11 @@ def convCombDeriv(file_path_in): for item in newComponents: doc.add(newComponents[item]) - # 7. Update the uri references + # 7. Update the variable features in each of the combinatorial derivation objects if there are conversions + if tempcombdev != combdev: + updateVariableFeatures(doc, combdev) + + # 8. Update the uri references oldToNew = {} for item in tempcombdev: @@ -115,179 +360,12 @@ def convCombDeriv(file_path_in): doc = convCombDeriv(file_path_in) +doc = updateLinearDNAProducts(doc) +doc = updateCollectionNames(doc) file_path_out = "SampleTemp3Output.nt" -doc.write(file_path_out) - - - -# collect all the objects which are combinatorial derivations and their featured components - -# knows_query = """ -# SELECT -# ?subject -# ?object -# WHERE { -# ?subject ?template . -# ?template ?feature . -# ?feature ?object . -# }""" -# qres = g.query(knows_query) - -# # create a tree structure -# children = set() # all objects that are referenced by another object -# parent_dict = {} # all objects that reference another object and the objects they reference as a set -# for row in qres: -# s = str(row.subject) -# o = str(row.object) -# children.add(o) -# if s in parent_dict: -# parent_dict[s].add(o) -# else: -# parent_dict[s] = {o} - -# parent_dict_copy = parent_dict - -# # objects that are only children are objects that are not also parents -# child_only = children - set(parent_dict).intersection(children) - -# # Loop through children create a dictionary for the object and the type of the object to look at in while loop -# # Keep track of the old uris and new uris in case they change - function already exists to change in document - -# old_uris = [] # Correlates index to index with each of the new uris -# new_uris = [] - -# # process the children only objects and update the 'tree' -# while len(parent_dict) > 0: -# # do something to deal with child_only -# # for this you probably need to read in the sbol document at the top and perform actions using pysbol - -# # Import SBOL document as the SBOL document type -# # Go through each of the children and determine if it is a combinatorial derivation - may have to save a copy of the parent dictionary -# # Go through each of the uris in the child object and look to see if those uris in parent dictionary are combinatorial derivations - going to need pysbol -# # Take it out as a string and check what the type is in the object in the sbol document -# # May have to write in another file for now -# # Object stays combinatorial derivation if one of its subcomponents is a combinatorial derivation -# # Else: Becomes a component -# # For collection: If has a variable feature and nothing else - -# # loop over parents - -# for parent in parent_dict: -# if parent_dict[parent].issubset(child_only): # If the parent's children are a subset of childOnly -# # print(parent) # Print the name of the parent object -# child_types = [] # Create a list of all of the types of child objects -# for child in parent_dict[parent]: -# children_of_parent = set() -# obj = doc.find(child) -# children_of_parent.add(type(obj)) - -# # if len(child_types) == len(parent_dict[parent]): # Check to see that it's the end of the loop -# # Trying to see if it can be a collection object -# if len(children_of_parent) == 0: # Checking to see if there aren't any children and only contains a variable feature -# parentObj = doc.find(parent) # In template not in the parent -# template = doc.find(f'{parent}_template') # Get the template object from the parent - -# if len(template.features) == 1 and type(template.features[0]) == sbol3.VariableFeature: # If it only has 1 variable feature and nothing else - collection object -# col = sbol3.Collection() -# for variant in template.features[0]: # Add all variants of the variable feature to the collection -# col.members.append(variant) - -# # Delete original comb der + template after adding in uris to appropriate arrays -# old_uris.append(parentObj.identity) -# new_uris.append(col.identity) - -# doc.remove_object(parentObj) -# doc.remove_object(template) +doc.write(file_path_out, file_format="sorted nt") - -# if sbol3.CombinatorialDerivation not in children_of_parent: # If there is no child that is a combinatorial derivation -# # Delete the template that's currently in the document -# # Create a new component object and carry over all of the features from the combinatorial derivation (including exact name) -# # Name, (references to) subcomponents, (references to) constraints, name, displayId, role, type -# # Delete the previous combinatorial derivation object -# # Put the new component object into the document - -# parentObj = doc.find(parent) # Get the object that the parent is supposed to be - -# template = doc.find(f'{parent}_template') # Get the template object from the parent - -# # print(parentObj._properties) -# old_uris.append(parentObj.identity) # Add the uri of the old parent object to the list - -# componentObj = sbol3.Component(identity=parentObj.identity, types=parentObj.type_uri) # Create a new component object - -# new_uris.append(componentObj.identity) # Add its URI to the list of new uris - -# componentObj.name = parentObj.name # Set the name - -# # print(parentObj._properties) - -# for feature in template.features: -# if type(feature) != sbol3.LocalSubComponent: -# subComp = sbol3.SubComponent(instance_of=feature.instance_of) -# componentObj.features.append(subComp) # - -# for role in template.roles: -# componentObj.roles.append(role) - -# # Still need to find out how to attribute subcomponents, constraints (Both subcomponents and constraints and roles in template) -# for prop in parentObj._properties: -# for subProp in parentObj._properties[prop]: -# if prop == "http://sbols.org/v3#template": -# continue - -# if type(subProp) == rdflib.term.URIRef: -# setattr(componentObj, prop, -# sbol3.URIProperty(componentObj, -# f'{prop}', -# '0', '*', initial_value=[subProp])) -# else: -# setattr(componentObj, prop, -# sbol3.TextProperty(componentObj, -# prop, -# '0', '*', initial_value=str(subProp))) - -# for constraint in template.constraints: -# newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject, constraint.object) -# newConstraint.derived_from = constraint.derived_from -# componentObj.constraints.append(newConstraint) -# # Add all to component -# # Eventually should be more filtered - -# componentObj.types[0] = sbol3.component.SBOL_COMPONENT - -# # print(componentObj._properties) - - -# doc.remove_object(parentObj) # Remove the old object -# doc.remove_object(template) # Remove the old template - -# doc.add(componentObj) # Add the new object - -# # print(child, parent_dict_copy[child]) - -# # remove child only children from dictionary -# new_parent_dict = {} - -# for key in parent_dict: -# current_children = parent_dict[key] -# remaining_children = current_children - current_children.intersection(child_only) -# if len(remaining_children) > 0: -# new_parent_dict[key] = remaining_children -# parent_dict = new_parent_dict - -# # update child_only list -# child_only = children - set(parent_dict).intersection(children) - -# # Update uris on document using the old uri and new uri comparison -# oldToNew = {} -# for i in range(len(old_uris)): -# oldToNew[old_uris[i]] = new_uris[i] - -# h.update_uri_refs(doc, oldToNew) - -# return updated sbol document From 3625e545ad89102929c871b0d92f9be8f3968168 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 5 Sep 2023 15:37:24 -0600 Subject: [PATCH 10/17] Changes to stabilize ordering of subcomponents in conversion --- temp3.py | 31 ++++++++++++++++++++++++------- 1 file changed, 24 insertions(+), 7 deletions(-) diff --git a/temp3.py b/temp3.py index 0b5ad59..cb275b2 100644 --- a/temp3.py +++ b/temp3.py @@ -231,6 +231,18 @@ def updateCollectionNames(doc): newCollection = sbol3.Collection(identity='CompositeParts', name=obj.name, members=obj.members, description=obj.description) removeList.append(obj) + # Change components inside of the composite parts collection to their inserted construct if they have one + + removeMember = [] + + for item in newCollection.members: + if doc.find(item + "_ins") != None: + removeMember.append(item) + newCollection.members.append(item + "_ins") + + for item in removeMember: + newCollection.members.remove(item) + addList.append(newCollection) for item in removeList: @@ -240,7 +252,6 @@ def updateCollectionNames(doc): doc.add(item) return doc - @@ -319,12 +330,22 @@ def convCombDeriv(file_path_in): # From template: features, constraints, type template = doc.find(f'{obj.identity}_template') - for feature in template.features: + # Need to go through the subcomponents in order from the previous template + # sort the subcomponents by the identity and then create a new list to run through it + + sortedSubcomponents = sorted(template.features, key=lambda x: x.identity) + + for feature in sortedSubcomponents: if type(feature) != sbol3.LocalSubComponent: subComp = sbol3.SubComponent(instance_of=feature.instance_of, orientation=feature.orientation) newComp.features.append(subComp) - for constraint in template.constraints: + # Attempt to sort the constraints in the same way as subcomponents + + sortedConstraints = sorted(template.constraints, key=lambda x: x.identity) + + + for constraint in sortedConstraints: newConstraint = sbol3.Constraint(constraint.restriction, constraint.subject.replace('_template', ''), constraint.object.replace('_template', '')) newConstraint.derived_from = constraint.derived_from newComp.constraints.append(newConstraint) @@ -365,7 +386,3 @@ def convCombDeriv(file_path_in): file_path_out = "SampleTemp3Output.nt" doc.write(file_path_out, file_format="sorted nt") - - - - From 41aa9da1ba4307c0ca0b0654a514d8c5e3f83770 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 5 Sep 2023 15:45:00 -0600 Subject: [PATCH 11/17] Edit to replace periods with underscores --- excelutils/excel_sbol_utils/helpers.py | 2 ++ 1 file changed, 2 insertions(+) diff --git a/excelutils/excel_sbol_utils/helpers.py b/excelutils/excel_sbol_utils/helpers.py index 42c5497..090c002 100644 --- a/excelutils/excel_sbol_utils/helpers.py +++ b/excelutils/excel_sbol_utils/helpers.py @@ -26,6 +26,8 @@ def check_name(nm_to_chck): elif ord(ltr) == 45: # Allow hyphens to be reinterpreted as underscores nm_to_chck = nm_to_chck.replace(ltr, "_") + elif ord(ltr) == 46: + nm_to_chck = nm_to_chck.replace(ltr, "_") elif ord(ltr) > 122 or ord(ltr) < 48: # 122 is the highest decimal code number # for common latin ltrs or arabic numbers From 1a9244faf25d7fc7c9bb1637461398b6b9917269 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 10 Oct 2023 15:17:15 -0600 Subject: [PATCH 12/17] Updates to give default descriptions to parts. --- temp3.py | 24 ++++++++++++++++++++++-- 1 file changed, 22 insertions(+), 2 deletions(-) diff --git a/temp3.py b/temp3.py index cb275b2..088b8e3 100644 --- a/temp3.py +++ b/temp3.py @@ -112,7 +112,7 @@ def updateVariableFeatures(doc, combdev): for feature in list(template.features): if type(feature) == sbol3.LocalSubComponent: - copyFeature = sbol3.LocalSubComponent(types=feature.type_uri, name=feature.name, orientation=feature.orientation) + copyFeature = sbol3.LocalSubComponent(types=feature.types, name=feature.name, orientation=feature.orientation) localsubs[int(feature.name.split(" ")[1])] = copyFeature template.features.remove(feature) elif type(feature) == sbol3.SubComponent: @@ -175,7 +175,7 @@ def updateVariableFeatures(doc, combdev): del feature._properties[sbol3.SBOL_NAME] - + # For every object in Composite parts collection: return None @@ -252,6 +252,25 @@ def updateCollectionNames(doc): doc.add(item) return doc + +# Go through the objects in the document and if they don't have a description, then add a blank +# Input: Document object (sbol3) +# Output: Document object with updated descriptions +# NOT COMPLETE - NEED MORE SPECIFIC IMPLEMENTATION + +def updateDescriptions(doc): + + for obj in doc.objects: + if type(obj) == sbol3.Collection: + for item in list(obj.members): + temp = doc.find(item) + + if "_ins" in temp.name: + continue + if temp.description == None: + temp.description = "" + + return doc @@ -383,6 +402,7 @@ def convCombDeriv(file_path_in): doc = convCombDeriv(file_path_in) doc = updateLinearDNAProducts(doc) doc = updateCollectionNames(doc) +doc = updateDescriptions(doc) file_path_out = "SampleTemp3Output.nt" doc.write(file_path_out, file_format="sorted nt") From ffb4eb0486e0ca0219d6f4d7cf5bcae17d5bc3d6 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 7 Dec 2023 16:18:51 -0700 Subject: [PATCH 13/17] Adding two backbones template, output (two_backbones.nt) and post-processing output (two_backnones_ud.nt) --- two_backbones.nt | 229 +++++++++++++++++++++++++++++++++++++++++++ two_backbones.xlsx | Bin 0 -> 173151 bytes two_backbones_ud.nt | 233 ++++++++++++++++++++++++++++++++++++++++++++ 3 files changed, 462 insertions(+) create mode 100644 two_backbones.nt create mode 100644 two_backbones.xlsx create mode 100644 two_backbones_ud.nt diff --git a/two_backbones.nt b/two_backbones.nt new file mode 100644 index 0000000..0005302 --- /dev/null +++ b/two_backbones.nt @@ -0,0 +1,229 @@ + "positive control 2018 interlab" . + "I20270" . + . + . + "I20270" . + . + . + . + "I20270_sequence" . + "TTGATGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "5.5 SLM (high, but variable)" . + "J23101" . + . + . + "J23101" . + . + . + . + "J23101_sequence" . + "TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC" . + . + . + . + "J23106 2018 interlab" . + "J364001" . + . + . + "J364001" . + . + . + . + "J364001_sequence" . + "TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23117 2018 interlab" . + "J364002" . + . + . + "J364002" . + . + . + . + "J364002_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23100 2018 interlab" . + "J364007" . + . + . + "J364007" . + . + . + . + "J364007_sequence" . + "TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23116 2018 interlab" . + "J364009" . + . + . + "J364009" . + . + . + . + "J364009_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "negative control 2018 interlab" . + "R0040" . + . + . + "R0040" . + . + . + . + "R0040_sequence" . + "TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCAC" . + . + . + . + "Backbone_variants" . + . + "Backbone variants" . + . + . + "SubComponent1" . + . + . + "Backbone_variants_ins" . + . + . + "Backbone variants insert" . + . + . + . + "Backbone_variants_template" . + . + . + . + "Collection from the Basic Parts sheet" . + "Basic_Parts" . + . + . + . + . + . + . + . + . + . + . + . + "Basic Parts" . + . + "Collection from the Composite Parts sheet" . + "Composite_Parts" . + . + . + . + "Composite Parts" . + . + "Final products desired for actual fabrication" . + "FinalProducts" . + . + . + . + "Final Products" . + . + "Linear DNA constructs to be fabricated" . + "LinearDNAProducts" . + . + . + . + "Linear DNA Products" . + . + . + "VariableFeature1" . + . + . + . + "Two_by_six" . + . + . + "Two by six" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + "Two_by_six_ins" . + . + . + "Two by six insert" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "Two_by_six_ins_template" . + . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "Two_by_six_template" . + . + . + . + . + . + . + . + "pOpen_v4" . + . + "pOpen_v4" . + . + . + . + . + "pMB1 replicon, 100-300 copy" . + "pSB1C3" . + . + "pSB1C3" . + . + . + . + . + "testfiles" . + . + "" . + . + . diff --git a/two_backbones.xlsx b/two_backbones.xlsx new file mode 100644 index 0000000000000000000000000000000000000000..09fbf0c0a245c8077e91f76dc693f035aa05b91e GIT binary patch literal 173151 zcmeEu1$3P`lCIe?Gcz+Y#F&_wV`gS%$IN!jj4?AaJ7$cTnVA`$lkVy19@yQtJF`3Q z?7Qb4UETUiQc0?kRQjY}MjRLf82}969RL6T0YC<i|M_3s0>kmkQk(Sf{-;sSf&KLbHc-t^g%oHV znkR&SaP+nL;fj5AM}Q0UtHIu;wUjg{QT>u7-_q@P)S`C7%HzXn(iuoMHAsZEWsvt` zXM0De&hO65tZ73&tTC&cRv=PIY?#_Q(|f9bN`NXiD+DK#tBWNmgu~=afZKC3T~-2a 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zHhsQ>b6Y39v@~<)bcWWod6}Phxd?Q8>YXE3grvXC8L2C_4hr!M&$v< z-q^sxfZ^f#z>)k4pwci+t`D>(`z;+))|I(DYM&N(;^1pV}2R|0SR^eB# zL_f>XqYUv2%5QxX{R;T2N0*;~i)#M_@Gt&dentG%*U3*r42^$+_|x;ruYkXL{`d)~ zruk0*fBFIW74TR07C!+ywEhX;PiGgu0{-d>;wRv?_OE~sulND#SCn6s-G8D;+x-^h zx1Ib~F7_wNS@5qYf9W9)SihZ~e`OXjFTcutKN03WKOp>_6!`1&e-)&D=I)Mr d$o;2SB_j?6%#pxC4*Z)27EtR1VB!V)e*jqX^e6xT literal 0 HcmV?d00001 diff --git a/two_backbones_ud.nt b/two_backbones_ud.nt new file mode 100644 index 0000000..f15e80a --- /dev/null +++ b/two_backbones_ud.nt @@ -0,0 +1,233 @@ + "positive control 2018 interlab" . + "I20270" . + . + . + "I20270" . + . + . + . + "I20270_sequence" . + "TTGATGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "5.5 SLM (high, but variable)" . + "J23101" . + . + . + "J23101" . + . + . + . + "J23101_sequence" . + "TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC" . + . + . + . + "J23106 2018 interlab" . + "J364001" . + . + . + "J364001" . + . + . + . + "J364001_sequence" . + "TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23117 2018 interlab" . + "J364002" . + . + . + "J364002" . + . + . + . + "J364002_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23100 2018 interlab" . + "J364007" . + . + . + "J364007" . + . + . + . + "J364007_sequence" . + "TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23116 2018 interlab" . + "J364009" . + . + . + "J364009" . + . + . + . + "J364009_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "negative control 2018 interlab" . + "R0040" . + . + . + "R0040" . + . + . + . + "R0040_sequence" . + "TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCAC" . + . + . + . + "Backbone_variants" . + . + "Backbone variants" . + . + . + . + "SubComponent1" . + . + . + "" . + "Backbone_variants_ins" . + . + . + "Backbone variants insert" . + . + . + . + "Backbone_variants_template" . + . + . + . + "Collection from the Basic Parts sheet" . + "BasicParts" . + . + . + . + . + . + . + . + . + . + . + . + "Basic Parts" . + . + "Collection from the Composite Parts sheet" . + "CompositeParts" . + . + . + . + "Composite Parts" . + . + "Final products desired for actual fabrication" . + "FinalProducts" . + . + . + . + "Final Products" . + . + "Linear DNA constructs to be fabricated" . + "LinearDNAProducts" . + . + . + . + "Linear DNA Products" . + . + . + "VariableFeature1" . + . + . + . + "Two_by_six" . + . + . + "Two by six" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + "" . + "Two_by_six_ins" . + . + . + "Two by six insert" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "Two_by_six_ins_template" . + . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "Two_by_six_template" . + . + . + . + . + . + . + . + "" . + "pOpen_v4" . + . + "pOpen_v4" . + . + . + . + . + "pMB1 replicon, 100-300 copy" . + "pSB1C3" . + . + "pSB1C3" . + . + . + . + . + "testfiles" . + . + "" . + . + . From cb76a9646eb3643bf0bc2a1f8830ce18ab8b3531 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Thu, 15 Feb 2024 11:11:17 -0700 Subject: [PATCH 14/17] Added implementation for multiple backbones. --- excelutils/excel_sbol_utils/library3.py | 125 +++++++++++++++++++----- excelutils/excel_sbol_utils/temp.py | 21 ++++ temp3.py | 36 +++++-- two_backbones.nt | 20 ++-- two_backbones.xlsx | Bin 173151 -> 173130 bytes two_backbones_ud.nt | 20 ++-- 6 files changed, 175 insertions(+), 47 deletions(-) create mode 100644 excelutils/excel_sbol_utils/temp.py diff --git a/excelutils/excel_sbol_utils/library3.py b/excelutils/excel_sbol_utils/library3.py index 4db8a5e..4ddf081 100644 --- a/excelutils/excel_sbol_utils/library3.py +++ b/excelutils/excel_sbol_utils/library3.py @@ -3,7 +3,7 @@ import logging import sbol3 import warnings -import excel_sbol_utils.helpers as helpers +import helpers as helpers def objectType(rowobj): @@ -34,6 +34,7 @@ def addToDescription(rowobj): 'different from': sbol3.SBOL_DIFFERENT_FROM, 'same orientation as': sbol3.SBOL_SAME_ORIENTATION_AS, 'different orientation from': sbol3.SBOL_SAME_ORIENTATION_AS} + def make_constraint(constraint, part_list, template): m = constraint_pattern.match(constraint) if not m: @@ -63,16 +64,58 @@ def subcomponents(rowobj): #UPDATE TO WORK WITH CELL DICT, ALLOW CONSTRAINTS if 'backbone' in rowobj.col_cell_dict: - temp = sbol3.Component(identity=f'{rowobj.obj.displayId}_ins_template', types=sbol3.SBO_DNA, name=f'{rowobj.obj.displayId}_ins_template') - newobj = sbol3.CombinatorialDerivation(identity=f'{rowobj.obj.displayId}_ins', template=temp, name=f'{rowobj.obj.displayId}_ins', strategy=sbol3.SBOL_ENUMERATE) - rowobj.doc.add(temp) - rowobj.doc.add(newobj) - rowobj.obj_dict[temp.display_id] = {'uri': temp.type_uri, 'object': temp, - 'displayId': temp.display_id} - backbones = list(rowobj.col_cell_dict['backbone'].values()) - back = True - oldobj = rowobj.obj - rowobj.obj = newobj + # If this row has a backbone, create a new combinatorial derivation + + # Determine if there are multiple comps per part + multiple = False + + for sub in rowobj.col_cell_dict['subcomp']: + if "," in rowobj.col_cell_dict['subcomp'][sub]: + multiple = True + break + else: + multiple = False + + # 1. If there are multiple comps per part, create ins_templat + + if multiple: + temp = sbol3.Component(identity=f'{rowobj.obj.displayId}_ins_template', types=sbol3.SBO_DNA) + + newobj = sbol3.CombinatorialDerivation(identity=f'{rowobj.obj.displayId}_ins', template=temp, name=f'{rowobj.obj.name} insert', \ + strategy=sbol3.SBOL_ENUMERATE, description=rowobj.obj.description) + + rowobj.obj.description = None + + rowobj.doc.add(temp) # Add the template + rowobj.doc.add(newobj) # Add the combdev _ins to the document connected to the template + + rowobj.obj_dict[temp.display_id] = {'uri': temp.type_uri, 'object': temp, + 'displayId': temp.display_id} + backbones = list(rowobj.col_cell_dict['backbone'].values()) + backbones = backbones[0].split(", ") + + back = True + oldobj = rowobj.obj + rowobj.obj = newobj + else: + # 2. Otherwise, create _ins without the template + + # Create new component _ins without the template + newobj = sbol3.Component(identity=f'{rowobj.obj.displayId}_ins', name=f'{rowobj.obj.name} insert', \ + description=rowobj.obj.description, types=sbol3.SBO_DNA, roles=sbol3.SO_ENGINEERED_REGION) + + # Set description to None + rowobj.obj.description = None + + rowobj.doc.add(newobj) + + backbones = list(rowobj.col_cell_dict['backbone'].values()) + backbones = backbones[0].split(", ") + + + back = True + oldobj = rowobj.obj + rowobj.obj = newobj else: back = False @@ -89,19 +132,40 @@ def subcomponents(rowobj): #UPDATE TO WORK WITH CELL DICT, ALLOW CONSTRAINTS variant_comps = [] comp_ind = 0 + # Need to update for multiple backbones, as well as remove hardcoding + # Check SBOL Utilities for reasoning for multiple backbones + if back: + # Currently this code creates a template for the insertion of the backbone into the main combinatorialderivation tempObj = rowobj.obj_dict[f'{oldobj.display_id}_template']['object'] - sub = sbol3.LocalSubComponent(types=sbol3.SBO_DNA, name="Inserted construct") + + sub = sbol3.LocalSubComponent(types=sbol3.SBO_DNA, name="Inserted Construct") tempObj.features.append(sub) backbone_sub = sbol3.VariableFeature(cardinality=sbol3.SBOL_ONE, variable=sub, variant_derivations=rowobj.obj) oldobj.variable_features.append(backbone_sub) - subComp = sbol3.SubComponent(instance_of=rowobj.obj_dict[backbones[0]]['object']) - rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].features.append(subComp) - constr1 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=subComp, subject=sub) - constr2 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=sub, subject=subComp) - rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr1) - rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr2) + if len(backbones) == 1: + + subComp = sbol3.SubComponent(instance_of=rowobj.obj_dict[backbones[0]]['object']) + rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].features.append(subComp) + constr1 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=subComp, subject=sub) + constr2 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=sub, subject=subComp) + rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr1) + rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr2) + else: + + newLocalSub = sbol3.LocalSubComponent(name="Vector", types=sbol3.SBO_DNA) + tempObj.features.append(newLocalSub) + + newVarFeature = sbol3.VariableFeature(variable=newLocalSub, variants=(rowobj.obj_dict[i]['object'] for i in backbones), cardinality=sbol3.SBOL_ONE) + oldobj.variable_features.append(newVarFeature) + + constr1 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=newLocalSub, subject=sub) + constr2 = sbol3.Constraint(restriction=sbol3.SBOL_MEETS, object=sub, subject=newLocalSub) + + rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr1) + rowobj.obj_dict[f'{oldobj.display_id}_template']['object'].constraints.append(constr2) + else: temp = rowobj.obj_dict[f'{rowobj.obj.display_id}_template']['object'] @@ -124,7 +188,7 @@ def subcomponents(rowobj): #UPDATE TO WORK WITH CELL DICT, ALLOW CONSTRAINTS comp_ind += 1 else: - tempSub = sbol3.SubComponent(name=f'Part {comp_ind}', instance_of=f'{rowobj.obj_dict[comp]["uri"]}', orientation=sbol3.SBOL_INLINE) + tempSub = sbol3.SubComponent(name=f'Part {comp_ind + 1}', instance_of=f'{rowobj.obj_dict[comp]["uri"]}', orientation=sbol3.SBOL_INLINE) temp.features.append(tempSub) variant_comps.append(tempSub) if comp_ind != 0: @@ -223,8 +287,8 @@ def sequence(rowobj): # create sequence object sequence = sbol3.Sequence(f"{rowobj.obj.namespace}/{rowobj.obj.display_id}_sequence", elements=val, encoding=sbol3.IUPAC_DNA_ENCODING, namespace=rowobj.obj.namespace) - if rowobj.obj.name is not None: - sequence.name = f"{rowobj.obj.name} Sequence" + # if rowobj.obj.name is not None: + # sequence.name = f"{rowobj.obj.name} Sequence" rowobj.doc.add(sequence) @@ -239,6 +303,11 @@ def sequence(rowobj): def circular(rowobj): # NOT IMPLEMENTED # if false add to linear collection if true add to types + + tempObj = rowobj.obj + if rowobj.col_cell_dict['Circular'] not in tempObj.types: + tempObj.types.append(rowobj.col_cell_dict['Circular']) + pass def finalProduct(rowobj): @@ -253,8 +322,9 @@ def finalProduct(rowobj): sbol_objs = doc.objects sbol_objs_names = [x.name for x in sbol_objs] - if 'FinalProducts' not in sbol_objs_names: - colec = sbol3.Collection('FinalProducts', name='FinalProducts') + if 'Final Products' not in sbol_objs_names: + colec = sbol3.Collection('FinalProducts', name='Final Products') + colec.description = 'Final products desired for actual fabrication' sbol_objs = doc.objects sbol_objs_names = [x.name for x in sbol_objs] @@ -262,11 +332,12 @@ def finalProduct(rowobj): doc.add(colec) colec.members.append(rowobj.obj_uri) else: - colec = sbol_objs[sbol_objs_names.index('FinalProducts')] + colec = sbol_objs[sbol_objs_names.index('Final Products')] colec.members.append(rowobj.obj_uri) - if 'LinearDNAProducts' not in sbol_objs_names: - colec = sbol3.Collection('LinearDNAProducts', name='LinearDNAProducts') + if 'Linear DNA Products' not in sbol_objs_names: + colec = sbol3.Collection('LinearDNAProducts', name='Linear DNA Products') + colec.description = 'Linear DNA constructs to be fabricated' sbol_objs = doc.objects sbol_objs_names = [x.name for x in sbol_objs] @@ -274,7 +345,7 @@ def finalProduct(rowobj): doc.add(colec) colec.members.append(rowobj.obj) else: - colec = sbol_objs[sbol_objs_names.index('LinearDNAProducts')] + colec = sbol_objs[sbol_objs_names.index('Linear DNA Products')] colec.members.append(rowobj.obj) diff --git a/excelutils/excel_sbol_utils/temp.py b/excelutils/excel_sbol_utils/temp.py new file mode 100644 index 0000000..046cf7a --- /dev/null +++ b/excelutils/excel_sbol_utils/temp.py @@ -0,0 +1,21 @@ +import sbol2 +import sbol3 +import excel_sbol_utils.library2 as l2 +import excel_sbol_utils.library3 as l3 +import excel_sbol_utils.helpers as help +import excel2sbol.comp_column_functions2 as ccf +import excel2sbol.converter as conv +import os + +# Build a barebones combinatorial derivation object +# Attempt to build subcomponent on it + +obj = sbol2.combinatorialderivation() + +doc = sbol2.Document() +obj_dict = {} +sheet = 'Composite Parts' + + + + diff --git a/temp3.py b/temp3.py index 088b8e3..7e6574a 100644 --- a/temp3.py +++ b/temp3.py @@ -4,7 +4,7 @@ import excel_sbol_utils.helpers as h direct = os.path.split(__file__)[0] -file_path_in = os.path.join(direct, 'SBOL3_simple_library4.nt') +file_path_in = os.path.join(direct, 'two_backbones.nt') # Purpose: After converting the combinatorial derivations, the references to the objects in other parts must be updated # Input: SBOL3 Document object, SBOL3 Dictionary of combinatorial derivations after conversion @@ -21,11 +21,12 @@ def updateVariableFeatures(doc, combdev): insert = item + "_ins" obj = doc.find(insert) - if obj == None: + if obj == None or type(obj) != sbol3.combderiv.CombinatorialDerivation: ins = False obj = doc.find(item) + obj.strategy = sbol3.SBOL_ENUMERATE - if ins: + if doc.find(f'{insert}_template'): template = doc.find(f'{insert}_template') else: template = doc.find(f'{item}_template') @@ -34,7 +35,21 @@ def updateVariableFeatures(doc, combdev): # 2: Go through every variable feature for variable_feature in list(obj.variable_features): - if len(variable_feature.variants) > 1: + # Go through and ensure that variantderivations are correct + + removeList = [] + addList = [] + for variant in variable_feature.variants: + if str(variant) in combdev: + addList.append(variant) + removeList.append(variant) + + for item in removeList: + variable_feature.variants.remove(item) + for item in addList: + variable_feature.variant_derivations.append(item) + + if len(variable_feature.variants) > 1 or len(variable_feature.variant_derivations) > 0: continue # Leave as a variable feature else: variant = variable_feature.variants[0] @@ -63,6 +78,7 @@ def updateVariableFeatures(doc, combdev): template.features.remove(localsub) obj.variable_features.remove(variable_feature) + if edited: @@ -93,7 +109,7 @@ def updateVariableFeatures(doc, combdev): # Copy the variable features and add them to copiedVariableFeatures - copyFeature = sbol3.VariableFeature(variants=variable_feature.variants, name=variable_feature.name, variable=variable_feature.variable, cardinality=variable_feature.cardinality) + copyFeature = sbol3.VariableFeature(variants=variable_feature.variants, variant_derivations=variable_feature.variant_derivations, name=variable_feature.name, variable=variable_feature.variable, cardinality=variable_feature.cardinality) copiedVariableFeatures[number] = copyFeature # Remove the variable feature from the object @@ -264,11 +280,14 @@ def updateDescriptions(doc): if type(obj) == sbol3.Collection: for item in list(obj.members): temp = doc.find(item) + if not temp: + continue if "_ins" in temp.name: continue if temp.description == None: - temp.description = "" + if doc.find(item + "_ins") == None: + temp.description = "" return doc @@ -305,7 +324,8 @@ def convCombDeriv(file_path_in): if ins in dictionaryObj: combdev[item] = dictionaryObj[item] tempcombdev.pop(item) - tempcombdev.pop(ins) + if ins in tempcombdev: + tempcombdev.pop(ins) # 2. Go through the remaining combinatorial derivation objects and check each variable feature to see if it has more than one variant @@ -404,5 +424,5 @@ def convCombDeriv(file_path_in): doc = updateCollectionNames(doc) doc = updateDescriptions(doc) -file_path_out = "SampleTemp3Output.nt" +file_path_out = "two_backbones_ud.nt" doc.write(file_path_out, file_format="sorted nt") diff --git a/two_backbones.nt b/two_backbones.nt index 0005302..ffcd86f 100644 --- a/two_backbones.nt +++ b/two_backbones.nt @@ -149,9 +149,16 @@ . . . + . + "VariableFeature2" . + . + . + . + . "Two_by_six" . . . + . "Two by six" . . . @@ -183,27 +190,28 @@ . . "Constraint1" . - . + . . . . "Constraint2" . . . - . + . . "LocalSubComponent1" . "Inserted Construct" . . . - "SubComponent1" . - . - . + "LocalSubComponent2" . + "Vector" . + . + . 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zuB03Xx(VM#gk|<1?9}9wZH$avLL4=^47x6^nBrDo58Z_QEaq81RqxS35SO*v28GmP zl~*Pz7m>=xRp=(qoG^&QTJUeIvr-X?k)!)fNN$Bar*Kb^euucmiul3YBD6`JxjFD` zR$U}R3!U<6@79;94EINJCshwj5Z5}BerN*<)0f#|SWiTumVpqL?-Hv08DCN|-1UpF zPp<|K>&?cs?c9iQ675cXYu`R+f!6Ve>IVT;wd=fAJtbxbx>M$%p88j#DetDzuc-x! zB%*!$B+h)gN5w?)@jc%N7hKnp60syIPoKq@3ziCRdQLztvKeZ(ug}DL*$_D$hPlcc z&zHoQv`|^k38!ib+Zg*`E^I{P&lSIOV>ZiY-%g^8PkEH&89u2kkD)hY75!MKEgupr z*q$;Zlfn(HtgU`q#jA6Qh8#~78IdsND`pz$SW6#zWk&WC@#2hBZLK}Z26}^4Tvc$g7+L*)3uEqI9#xIRx!NzZRx`M!{ZRBID z!b>UiBEBbgsop@Y0lKZ?V>j&Ir@LwSnZ5J~6n-|D^m_RP!KuG>z4gGAt8INRVCxhN z7+=Koah_C)qkArNWu?tjBoEO164c$45UF%iP70e?=No%-OiUyGWn*WlqRx`drGgbZ zvkNT)81c5gcWTdbNZ;>Q&{M$fZ}jWl`j9$d+LV9@Y8k=PZJw)p+L0cqD5KuVhg;Zs zLVi&m;C8IjWJH~U8-rc;ro(afUY==Y*Q0IR=zElrAz>?6$;?N~kDhk9eF?&J`MS+{ zm#oFVdBx|Q$vujJi4&e5w^a8lImH!@u#hbd4^4&PR=B}oveH%`61{S_`G!%lri$*~qg|8uCj1-YGrjy*8C@AaK(Erp5&&|NviA?~P{s<$a z_?x{h3JTpn@afQm!67}cR|Y1$_pZ$7{P9Go3wF+HA7Lz4Za@7ah$k5k=;&QO(Zk;) zgSb}xKjMaxVWi}Lvy%LC6RjG+)ly)DNW)1&vcD?zr~XW)0#{9eu~PhPwf}Qr_rw0k zk1ZS+Fw~^LaLN9*_M!Z#g=FAMDKH7tJUDYIjPCbnhO4E*2vLXOW~neSR9kp@Du|zi WFQ>vpQQP4RX)wxb_6gt}qx>Ic=h5Z> diff --git a/two_backbones_ud.nt b/two_backbones_ud.nt index f15e80a..6d7d5e6 100644 --- a/two_backbones_ud.nt +++ b/two_backbones_ud.nt @@ -151,9 +151,16 @@ . . . + . + "VariableFeature2" . + . + . + . + . "Two_by_six" . . . + . "Two by six" . . . @@ -186,27 +193,28 @@ . . "Constraint1" . - . + . . . . "Constraint2" . . . - . + . . "LocalSubComponent1" . "Inserted Construct" . . . - "SubComponent1" . - . - . + "LocalSubComponent2" . + "Vector" . + . + . "Two_by_six_template" . . . . - . + . . . . From 4a8ade00e10133ca63fd463b207179317e1e8687 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 7 May 2024 12:18:45 -0600 Subject: [PATCH 15/17] Recent updates to temp3 and two_backbones --- SBOL3_simple_library4.nt | 544 ++++++++++++++++------------- SBOL3_simple_library4.xlsx | Bin 192074 -> 184176 bytes simple_library.nt | 685 +++++++++++++++++++++++++++++++++++++ temp.py | 11 +- temp3.py | 18 +- two_backbones.nt | 33 +- two_backbones.xlsx | Bin 173130 -> 174806 bytes two_backbones_ud.nt | 35 +- 8 files changed, 1031 insertions(+), 295 deletions(-) create mode 100644 simple_library.nt diff --git a/SBOL3_simple_library4.nt b/SBOL3_simple_library4.nt index 18df532..72a61cf 100644 --- a/SBOL3_simple_library4.nt +++ b/SBOL3_simple_library4.nt @@ -1,107 +1,176 @@ - "GFPmut3" . + "Green FP (off patent)\nGFPmut3" . "E0040" . . - . - . + . + "GFPmut3" . + . . . - "Green FP (off patent)" . - "mRFP1" . + "E0040_sequence" . + "ATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAA" . + . + . + . + "Red FP (off patent)\nmRFP1" . "E1010" . . - . - . + . + "mRFP1" . + . . . - "Red FP (off patent)" . + "E1010_sequence" . + "ATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGC" . + . + . + . + "positive control 2018 interlab" . "I20270" . . - . - . + . + "I20270" . + . . . - "positive control 2018 interlab" . - "mCherry" . + "I20270_sequence" . + "TTGATGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Red FP (off patent)\nmCherry" . "J06504" . . - . - . + . + "J06504" . + . . . - "Red FP (off patent)" . + "J06504_sequence" . + "ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCTTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG" . + . + . + . + "5.5 SLM (high, but variable)" . "J23101" . . - . - . + . + "J23101" . + . . . - "5.5 SLM (high, but variable)" . + "J23101_sequence" . + "TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC" . + . + . + . + "TBD SLM (expect 4.5)" . "J23105" . . - . - . + . + "J23105" . + . . . - "TBD SLM (expect 4.5)" . + "J23105_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGC" . + . + . + . + "J23106 2018 interlab" . "J364001" . . - . - . + . + "J364001" . + . . . - "J23106 2018 interlab" . + "J364001_sequence" . + "TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23117 2018 interlab" . "J364002" . . - . - . + . + "J364002" . + . . . - "J23117 2018 interlab" . + "J364002_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23100 2018 interlab" . "J364007" . . - . - . + . + "J364007" . + . . . - "J23100 2018 interlab" . + "J364007_sequence" . + "TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23116 2018 interlab" . "J364009" . . - . - . + . + "J364009" . + . . . - "J23116 2018 interlab" . - "TagBFP2" . + "J364009_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Blue FP\nTagBFP2" . "K592100" . . - . - . + . + "K592100" . + . . . - "Blue FP" . + "K592100_sequence" . + "ATGAGCGAACTGATCAAAGAGAACATGCACATGAAGCTGTACATGGAAGGCACCGTTGACAACCACCACTTTAAGTGCACGTCTGAGGGTGAGGGTAAGCCGTACGAAGGCACCCAAACCATGCGTATCAAAGTTGTGGAGGGCGGTCCACTGCCGTTCGCTTTTGACATTCTGGCGACCAGCTTCCTGTACGGTTCCAAAACGTTCATTAACCATACTCAGGGCATTCCGGATTTCTTCAAACAGAGCTTTCCGGAAGGTTTCACCTGGGAGCGTGTCACCACGTATGAAGATGGTGGTGTGTTGACCGCCACCCAAGATACCTCCCTGCAAGATGGCTGTCTGATCTATAACGTGAAAATTCGTGGCGTCAACTTTACGAGCAATGGTCCGGTGATGCAGAAGAAAACCCTGGGTTGGGAGGCGTTTACGGAAACCCTGTATCCGGCCGATGGTGGCCTGGAGGGCCGTAACGACATGGCACTGAAGCTGGTTGGTGGCAGCCATTTGATCGCAAATATCAAGACGACGTACCGCAGCAAGAAACCGGCGAAAAATCTGAAGATGCCGGGTGTTTACTATGTCGACTACCGTCTGGAACGCATTAAAGAAGCGAATAATGAGACTTACGTGGAGCAGCACGAGGTTGCAGTCGCGCGCTATTGCGACTTGCCTAGCAAGCTGGGTCATAAACTGAAT" . + . + . + . + "negative control 2018 interlab" . "R0040" . . - . - . + . + "R0040" . + . . . - "negative control 2018 interlab" . + "R0040_sequence" . + "TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCAC" . + . + . + . . "VariableFeature1" . . - . - . + . + . + . + . . - . - . - . . . . + . . "All_FPs" . . . + "All FPs" . . . "LocalSubComponent1" . @@ -117,144 +186,148 @@ "B0015" . . . - . + "B0015" . + . . . "B0015_sequence" . - "ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . + "CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . . . + "Moderate (?)" . "B0032" . . . - . + "B0032" . + . . . - "Moderate (?)" . "B0032_sequence" . - "tcacacaggaaag" . + "TCACACAGGAAAG" . + . . . - "5' UTR including B0032 RBS and BB scars (long and short)" . - "BB_u45_B0032_u45_BB" . - . - . - . - "Constraint1" . - . - . - . - . - "Constraint2" . - . - . - . - . - "SubComponent1" . - . - "Part 0" . - . - . - "SubComponent2" . - . - "Part 1" . - . - . - "SubComponent3" . - . - "Part 2" . - . - . - "BB_u45_B0032_u45_BB_template" . - . - . - . - . - . - . - . - . - "Collection from the Basic Parts sheet" . + "5' UTR including B0032 RBS and BB scars (long and short)" . + "BB_B0032_BB" . + . + "BB-B0032-BB" . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "SubComponent1" . + . + "Part 1" . + . + . + "SubComponent2" . + . + "Part 2" . + . + . + "SubComponent3" . + . + "Part 3" . + . + . + "BB_B0032_BB_template" . + . + . + . + . + . + . + . + . + "Designs for round 1 if iGEM 2021 calibration experimentation" . "Basic_Parts" . . + . + . + . + . + . + . + . + . + . + . + . + . . . . . - . - . . - . - . - . - . - . - . - . - . - . - . - . . . . - . . . . . + . + . + "Fluorescence calibration basic parts" . . + "BioBricks scar including ag (not before CDS)" . "BioBricks_scar_long" . . . - . + "BioBricks_scar_long" . + . . . - "BioBricks scar including ag (not before CDS)" . "BioBricks_scar_long_sequence" . - "tactagag" . + "TACTAGAG" . + . . . + "BioBricks scar (short one, w/o ag, before CDS)" . "BioBricks_scar_short" . . . - . + "BioBricks_scar_short" . + . . . - "BioBricks scar (short one, w/o ag, before CDS)" . "BioBricks_scar_short_sequence" . - "tactag" . + "TACTAG" . + . . . - "Collection from the Composite Parts sheet" . + "Designs for round 1 if iGEM 2021 calibration experimentation" . "Composite_Parts" . . . - . + . . . - . + . . + "Fluorescence calibration devices" . . - "E0040_sequence" . - "atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa" . - . - . - "E1010_sequence" . - "atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc" . - . - . - "ECFP" . + "Cyan FP; codon optimized using IDT tool \nECFP" . "ECFP" . . . - . + "ECFP" . + . . . . - "Cyan FP; codon optimized using IDT tool " . "ECFP_sequence" . - "atggttagtaaaggggaagagttgtttacgggcgttgttccaatcttggtggagttggacggggacgttaatgggcacaaattttcggtctctggggaaggcgagggggacgccacgtatggtaagctgaccttgaagttcatttgcactactgggaaattgccggttccctggcctacgcttgttaccacgttgacctggggcgtacaatgtttctcgcgctatcctgaccacatgaaacaacatgatttctttaagtcagctatgcctgagggctacgtgcaggagcgcactatcttcttcaaggatgacgggaattacaaaacccgtgcggaggtaaagttcgagggtgacacgcttgtcaaccgcatcgagttgaagggcattgattttaaggaggatggcaacattttaggacataaattagagtataactatatttcgcataatgtctacattactgctgacaaacaaaagaacggcatcaaggctaatttcaaaattcgccacaacatcgaggatggatcggttcagttagcagatcattaccagcaaaacacgcccatcggtgacggaccagttctgttacctgacaaccactatctttctacacagagtgctcttagcaaggaccctaacgaaaaacgtgaccacatggtcctgctggagtttgtgacagccgcaggtattacattggggatggacgaattgtataag" . + "ATGGTTAGTAAAGGGGAAGAGTTGTTTACGGGCGTTGTTCCAATCTTGGTGGAGTTGGACGGGGACGTTAATGGGCACAAATTTTCGGTCTCTGGGGAAGGCGAGGGGGACGCCACGTATGGTAAGCTGACCTTGAAGTTCATTTGCACTACTGGGAAATTGCCGGTTCCCTGGCCTACGCTTGTTACCACGTTGACCTGGGGCGTACAATGTTTCTCGCGCTATCCTGACCACATGAAACAACATGATTTCTTTAAGTCAGCTATGCCTGAGGGCTACGTGCAGGAGCGCACTATCTTCTTCAAGGATGACGGGAATTACAAAACCCGTGCGGAGGTAAAGTTCGAGGGTGACACGCTTGTCAACCGCATCGAGTTGAAGGGCATTGATTTTAAGGAGGATGGCAACATTTTAGGACATAAATTAGAGTATAACTATATTTCGCATAATGTCTACATTACTGCTGACAAACAAAAGAACGGCATCAAGGCTAATTTCAAAATTCGCCACAACATCGAGGATGGATCGGTTCAGTTAGCAGATCATTACCAGCAAAACACGCCCATCGGTGACGGACCAGTTCTGTTACCTGACAACCACTATCTTTCTACACAGAGTGCTCTTAGCAAGGACCCTAACGAAAAACGTGACCACATGGTCCTGCTGGAGTTTGTGACAGCCGCAGGTATTACATTGGGGATGGACGAATTGTATAAG" . + . . . . @@ -265,23 +338,24 @@ "FPs_small" . . . + "FPs small" . . . . "VariableFeature1" . . - . + . . . "VariableFeature2" . . - . - . + . + . . . "VariableFeature3" . . - . + . . . "VariableFeature4" . @@ -294,7 +368,7 @@ . . . - "FPs_small_ins" . + "FPs small insert" . . . . @@ -345,12 +419,12 @@ . "SubComponent1" . . - "Part 4" . + "Part 5" . . . "SubComponent2" . . - "Part 5" . + "Part 6" . . . "FPs_small_ins_template" . @@ -366,7 +440,6 @@ . . . - "FPs_small_ins_template" . . . "Constraint1" . @@ -380,7 +453,7 @@ . . "LocalSubComponent1" . - "Inserted construct" . + "Inserted Construct" . . . "SubComponent1" . @@ -394,58 +467,20 @@ . . . + "Final products desired for actual fabrication" . "FinalProducts" . . . . - "FinalProducts" . + "Final Products" . . - "I20270_sequence" . - "ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . - . - . - "J06504_sequence" . - "atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag" . - . - . - "J23101_sequence" . - "tttacagctagctcagtcctaggtattatgctagc" . - . - . - "J23105_sequence" . - "ttgacagctagctcagtcctagggactatgctagc" . - . - . - "J364001_sequence" . - "tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . - . - . - "J364002_sequence" . - "ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . - . - . - "J364007_sequence" . - "ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . - . - . - "J364009_sequence" . - "ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" . - . - . - "K592100_sequence" . - "atgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaat" . - . - . + "Linear DNA constructs to be fabricated" . "LinearDNAProducts" . . . . - "LinearDNAProducts" . + "Linear DNA Products" . . - "R0040_sequence" . - "tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac" . - . - . . "VariableFeature1" . . @@ -455,6 +490,7 @@ "Round_1_order" . . . + "Round 1 order" . . . "LocalSubComponent1" . @@ -467,62 +503,69 @@ . . . + "2 stop codons" . "STOP" . . . - . + "STOP" . + . . . - "2 stop codons" . "STOP_sequence" . - "taataa" . + "TAATAA" . + . . . "UNSX" . . . - . + "UNSX" . + . . . + "UNSX_UP" . + . + "UNSX-UP" . + . + . + "Constraint1" . + . + . + . + . + "SubComponent1" . + . + "Part 1" . + . + . + "SubComponent2" . + . + "Part 2" . + . + . + "UNSX_UP_template" . + . + . + . + . + . + . "UNSX_sequence" . - "ccaggatacatagattaccacaactccgagcccttccacc" . + "CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC" . + . . . - "UNSX_u45_UP" . - . - . - . - "Constraint1" . - . - . - . - . - "SubComponent1" . - . - "Part 0" . - . - . - "SubComponent2" . - . - "Part 1" . - . - . - "UNSX_u45_UP_template" . - . - . - . - . - . - . + "consensus without runs of As and Ts -59 to -36" . "UP_element_consensus" . . . - . + "UP element consensus" . + . . . - "consensus without runs of As and Ts -59 to -36 https://www.pnas.org/content/pnas/95/17/9761.full.pdf" . "UP_element_consensus_sequence" . - "tgaaatatattttcgaaaagccgc" . + "TGAAATATATTTTCGAAAAGCCGC" . + . . . . @@ -533,23 +576,24 @@ "_2018_Interlab" . . . + "2018 Interlab" . . . - "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . . "VariableFeature1" . . - . - . - . - . - . - . + . + . + . + . + . + . . + "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . "_2018_Interlab_ins" . . . - "_2018_Interlab_ins" . + "2018 Interlab insert" . . . . @@ -561,7 +605,6 @@ "_2018_Interlab_ins_template" . . . - "_2018_Interlab_ins_template" . . . "Constraint1" . @@ -575,7 +618,7 @@ . . "LocalSubComponent1" . - "Inserted construct" . + "Inserted Construct" . . . "SubComponent1" . @@ -589,65 +632,72 @@ . . . - "mCerulean3_sequence" . - "atggtgtcgaaaggtgaagaactttttacaggtgtggtccccattttagtcgaattagatggggatgtgaatggtcacaaattctcggtgtctggggagggtgaaggtgacgcgacctacgggaagttgactctgaaattcatttgcactacggggaagttaccagtgccctggcccactctggttacaacactgtcatggggcgtccaatgttttgctcgttatcctgaccacatgaaacagcatgacttcttcaagagcgcaatgccggaaggctatgtccaagagcgtactatctttttcaaagacgatgggaactacaaaacacgcgcagaagtgaagtttgagggagataccctggttaatcgcattgaattgaaaggtatcgacttcaaggaggatggtaatatcctggggcacaaattggagtacaacgctatccacggcaatgtttacattacggcagacaaacagaaaaacggaattaaggccaatttcggacttaactgcaacatcgaagatgggtcggtacaactggcagaccactatcagcaaaatacgcctattggtgacggtcctgtcctgttaccggacaatcattatctttccactcagtcgaaactgagtaaagatccgaacgagaagcgcgatcacatggtattattagagttcgtcacagccgcaggtattacgttagggatggatgagctttacaag" . - . - . + "Blue FP codon optimized" . "mTagBFP2" . . . - . + "mTagBFP2" . + . . . - "Blue FP codon optimized" . "mTagBFP2_sequence" . - "atggtatcgaagggtgaagagttgattaaggaaaacatgcacatgaagttatacatggaagggaccgtggataatcaccatttcaaatgcacatctgaaggagagggaaaaccttacgaagggacccagacaatgcgtatcaaagtggtcgagggtggccctttaccattcgcgttcgacatcctggcgacctcctttttatatgggagcaaaacattcattaaccacacccagggaatcccggatttcttcaagcagtctttccctgaagggtttacgtgggagcgtgtgacaacttatgaggatggcggggttttgacggctacgcaggatacttcgttacaagacggttgcttaatttacaatgtaaagattcgtggagtaaattttacatcaaacggtccggtcatgcaaaagaaaacccttggttgggaggcgtttaccgagacattgtatcctgccgacgggggattagagggtcgcaatgatatggcactgaaacttgtgggggggtctcatttaattgcaaacgctaagacaacttaccgctccaagaagcccgctaagaacctgaaaatgcctggggtctactatgttgactatcgccttgagcgtatcaaggaggcgaacaacgagacttacgtcgagcagcatgaggtagcggttgcccgttactgtgacctgccatctaagttaggacataagttgaat" . + "ATGGTATCGAAGGGTGAAGAGTTGATTAAGGAAAACATGCACATGAAGTTATACATGGAAGGGACCGTGGATAATCACCATTTCAAATGCACATCTGAAGGAGAGGGAAAACCTTACGAAGGGACCCAGACAATGCGTATCAAAGTGGTCGAGGGTGGCCCTTTACCATTCGCGTTCGACATCCTGGCGACCTCCTTTTTATATGGGAGCAAAACATTCATTAACCACACCCAGGGAATCCCGGATTTCTTCAAGCAGTCTTTCCCTGAAGGGTTTACGTGGGAGCGTGTGACAACTTATGAGGATGGCGGGGTTTTGACGGCTACGCAGGATACTTCGTTACAAGACGGTTGCTTAATTTACAATGTAAAGATTCGTGGAGTAAATTTTACATCAAACGGTCCGGTCATGCAAAAGAAAACCCTTGGTTGGGAGGCGTTTACCGAGACATTGTATCCTGCCGACGGGGGATTAGAGGGTCGCAATGATATGGCACTGAAACTTGTGGGGGGGTCTCATTTAATTGCAAACGCTAAGACAACTTACCGCTCCAAGAAGCCCGCTAAGAACCTGAAAATGCCTGGGGTCTACTATGTTGACTATCGCCTTGAGCGTATCAAGGAGGCGAACAACGAGACTTACGTCGAGCAGCATGAGGTAGCGGTTGCCCGTTACTGTGACCTGCCATCTAAGTTAGGACATAAGTTGAAT" . + . . . - "meffCFP" . + "Cyan FP (free); codon optimized using IDT tool \nmeffCFP" . "meffCFP" . . . - . + "meffCFP" . + . . . . - "Cyan FP (free); codon optimized using IDT tool " . "meffCFP_sequence" . - "atggcgctttcaaagcagagtttaccgagtgacatgaagttgatctaccacatggacgggaacgtgaatggtcactctttcgtcattaagggtgaaggcgagggaaaaccctatgaaggaacacatactatcaagctgcaagttgttgagggctcaccgttacctttcagcgccgacattctgtccacagtttttcaatatgggaaccgctgtttcactaagtatccgcctaatatcgtcgattattttaagaatagctgttctggtgggggctacaagtttggacgcagctttctttacgaagacggtgcggtctgtacggcgtcaggcgatattactctgtccgcagacaaaaagagctttgaacacaaatcgaaattcttaggcgtgaacttcccggctgacggtccggtgatgaagaaagagaccaccaactgggaaccatcgtgcgaaaaaatgactccgaatggcatgacactgatcggtgacgttacgggttttttgttgaaggaggacggaaagcgttacaaatgtcaatttcataccttccatgacgctaaagataagtccaaaaagatgccgatgcccgacttccactttgtccagcataaaattgagcgcaaggaccttcctggaagtatgcaaacatggcgtcttaccgagcacgcggcagcatgtaaaacttgttttacggag" . + "ATGGCGCTTTCAAAGCAGAGTTTACCGAGTGACATGAAGTTGATCTACCACATGGACGGGAACGTGAATGGTCACTCTTTCGTCATTAAGGGTGAAGGCGAGGGAAAACCCTATGAAGGAACACATACTATCAAGCTGCAAGTTGTTGAGGGCTCACCGTTACCTTTCAGCGCCGACATTCTGTCCACAGTTTTTCAATATGGGAACCGCTGTTTCACTAAGTATCCGCCTAATATCGTCGATTATTTTAAGAATAGCTGTTCTGGTGGGGGCTACAAGTTTGGACGCAGCTTTCTTTACGAAGACGGTGCGGTCTGTACGGCGTCAGGCGATATTACTCTGTCCGCAGACAAAAAGAGCTTTGAACACAAATCGAAATTCTTAGGCGTGAACTTCCCGGCTGACGGTCCGGTGATGAAGAAAGAGACCACCAACTGGGAACCATCGTGCGAAAAAATGACTCCGAATGGCATGACACTGATCGGTGACGTTACGGGTTTTTTGTTGAAGGAGGACGGAAAGCGTTACAAATGTCAATTTCATACCTTCCATGACGCTAAAGATAAGTCCAAAAAGATGCCGATGCCCGACTTCCACTTTGTCCAGCATAAAATTGAGCGCAAGGACCTTCCTGGAAGTATGCAAACATGGCGTCTTACCGAGCACGCGGCAGCATGTAAAACTTGTTTTACGGAG" . + . . . - "mmilCFP" . + "Blue FP (free); codon optimized using IDT tool \nmmilCFP" . "mmilCFP" . . . - . + "mmilCFP" . + . . . . - "Blue FP (free); codon optimized using IDT tool " . "mmilCFP_sequence" . - "atggctctgcctaaacaaatgaaactgacttatcacatggagggtactgtgaacggtcatttttttatcatcaagggcgaaggagggggagagccgtatgagggtactcatacgatcaagttacaggtggtagagggaagcccgttgcccttttcaccggatattctttccaccgtcttccaatacggcaaccgctgcttcactaaataccctcccaacatcgttgattacttcaaaaactcctgctcgggcggcggttatacttttggacgtagcttcctttatgaggacggagctgtctgtactgcttccggtgacattacgctgagcagcgacaaatcatcctttgagcacaaatctaaatttctgggagtaaactttcctgccgatggcccggtgatgaaaaaagaaaccactaattgggagccttcatgtgagaaaatgacacccaacgggatgaccttaatcggtgatgtgacggagttcctgttgaagaaggacggtaagcgttacaagtgccaattccacacattccacgacgcgaaggagaaaagccgcaatatgccgatgcccgatttccatttcgttcagcacgagattgaacgtaaagatttgccaggaccaatgcaaacatggcagttgaccgagcacgcagcagcctgtaagaactgtttcacagag" . + "ATGGCTCTGCCTAAACAAATGAAACTGACTTATCACATGGAGGGTACTGTGAACGGTCATTTTTTTATCATCAAGGGCGAAGGAGGGGGAGAGCCGTATGAGGGTACTCATACGATCAAGTTACAGGTGGTAGAGGGAAGCCCGTTGCCCTTTTCACCGGATATTCTTTCCACCGTCTTCCAATACGGCAACCGCTGCTTCACTAAATACCCTCCCAACATCGTTGATTACTTCAAAAACTCCTGCTCGGGCGGCGGTTATACTTTTGGACGTAGCTTCCTTTATGAGGACGGAGCTGTCTGTACTGCTTCCGGTGACATTACGCTGAGCAGCGACAAATCATCCTTTGAGCACAAATCTAAATTTCTGGGAGTAAACTTTCCTGCCGATGGCCCGGTGATGAAAAAAGAAACCACTAATTGGGAGCCTTCATGTGAGAAAATGACACCCAACGGGATGACCTTAATCGGTGATGTGACGGAGTTCCTGTTGAAGAAGGACGGTAAGCGTTACAAGTGCCAATTCCACACATTCCACGACGCGAAGGAGAAAAGCCGCAATATGCCGATGCCCGATTTCCATTTCGTTCAGCACGAGATTGAACGTAAAGATTTGCCAGGACCAATGCAAACATGGCAGTTGACCGAGCACGCAGCAGCCTGTAAGAACTGTTTCACAGAG" . + . . . + "pMB1 replicon, 100-300 copy" . "pSB1C3" . . - . + "pSB1C3" . + . . + . . - "pMB1 replicon, 100-300 copy" . "LmrA" . . - . + "LmrA" . . . . - "mCerulean3" . - "ATP07149_u46_1" . - . - . - . - . - . - . - "Cyan FP; codon optimized using IDT tool " . + "Cyan FP; codon optimized using IDT tool \nmCerulean3" . + "ATP07149_1" . + . + . + "mCerulean3" . + . + . + . + . + "ATP07149_1_sequence" . + "ATGGTGTCGAAAGGTGAAGAACTTTTTACAGGTGTGGTCCCCATTTTAGTCGAATTAGATGGGGATGTGAATGGTCACAAATTCTCGGTGTCTGGGGAGGGTGAAGGTGACGCGACCTACGGGAAGTTGACTCTGAAATTCATTTGCACTACGGGGAAGTTACCAGTGCCCTGGCCCACTCTGGTTACAACACTGTCATGGGGCGTCCAATGTTTTGCTCGTTATCCTGACCACATGAAACAGCATGACTTCTTCAAGAGCGCAATGCCGGAAGGCTATGTCCAAGAGCGTACTATCTTTTTCAAAGACGATGGGAACTACAAAACACGCGCAGAAGTGAAGTTTGAGGGAGATACCCTGGTTAATCGCATTGAATTGAAAGGTATCGACTTCAAGGAGGATGGTAATATCCTGGGGCACAAATTGGAGTACAACGCTATCCACGGCAATGTTTACATTACGGCAGACAAACAGAAAAACGGAATTAAGGCCAATTTCGGACTTAACTGCAACATCGAAGATGGGTCGGTACAACTGGCAGACCACTATCAGCAAAATACGCCTATTGGTGACGGTCCTGTCCTGTTACCGGACAATCATTATCTTTCCACTCAGTCGAAACTGAGTAAAGATCCGAACGAGAAGCGCGATCACATGGTATTATTAGAGTTCGTCACAGCCGCAGGTATTACGTTAGGGATGGATGAGCTTTACAAG" . + . + . + . diff --git a/SBOL3_simple_library4.xlsx b/SBOL3_simple_library4.xlsx index 269a7adde441a6df90d9ba787dd4fde826e5e7fd..022f8e4f43183887eeda58a26ad39ed4eb04b814 100644 GIT binary patch literal 184176 zcmeEt^9&!bazNdcc*}WARsB--QA5KHFS4LNeM`INw?B@KZAbvKIfi& 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ziaG0qd9Z*%$*fps8uNL(X!D%`oh(fq;SaQ(fn|giPUy<46mxG>j2Z+ImO@RA6J0Qh zOXm%WnjCklC3zizBy|HwKUceh;~B--5^f9UJ^MV%2vmT0E{g{j5auzUwkglA0yrZ< zpm&20-PLp(zkN{z6zj>~Z)cv9Ts_fZMA1>08`7g2f!^b*3Q)l32;l5)*crvi839b| zp)(E9utK~6Y&I}Z!>JgD)&UW86z-_K?y6%DsE`{QrS5sM&{BlZQBe1HI=>R?ObEKB zJ--8?= "Green FP (off patent)\nGFPmut3" . + "E0040" . + . + . + "GFPmut3" . + . + . + . + "E0040_sequence" . + "ATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAA" . + . + . + . + "Red FP (off patent)\nmRFP1" . + "E1010" . + . + . + "mRFP1" . + . + . + . + "E1010_sequence" . + "ATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGC" . + . + . + . + "positive control 2018 interlab" . + "I20270" . + . + . + "I20270" . + . + . + . + "I20270_sequence" . + "TTGATGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Red FP (off patent)\nmCherry" . + "J06504" . + . + . + "J06504" . + . + . + . + "J06504_sequence" . + "ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCTTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG" . + . + . + . + "5.5 SLM (high, but variable)" . + "J23101" . + . + . + "J23101" . + . + . + . + "J23101_sequence" . + "TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC" . + . + . + . + "TBD SLM (expect 4.5)" . + "J23105" . + . + . + "J23105" . + . + . + . + "J23105_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGC" . + . + . + . + "J23106 2018 interlab" . + "J364001" . + . + . + "J364001" . + . + . + . + "J364001_sequence" . + "TTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23117 2018 interlab" . + "J364002" . + . + . + "J364002" . + . + . + . + "J364002_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23100 2018 interlab" . + "J364007" . + . + . + "J364007" . + . + . + . + "J364007_sequence" . + "TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "J23116 2018 interlab" . + "J364009" . + . + . + "J364009" . + . + . + . + "J364009_sequence" . + "TTGACAGCTAGCTCAGTCCTAGGGACTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Blue FP\nTagBFP2" . + "K592100" . + . + . + "K592100" . + . + . + . + "K592100_sequence" . + "ATGAGCGAACTGATCAAAGAGAACATGCACATGAAGCTGTACATGGAAGGCACCGTTGACAACCACCACTTTAAGTGCACGTCTGAGGGTGAGGGTAAGCCGTACGAAGGCACCCAAACCATGCGTATCAAAGTTGTGGAGGGCGGTCCACTGCCGTTCGCTTTTGACATTCTGGCGACCAGCTTCCTGTACGGTTCCAAAACGTTCATTAACCATACTCAGGGCATTCCGGATTTCTTCAAACAGAGCTTTCCGGAAGGTTTCACCTGGGAGCGTGTCACCACGTATGAAGATGGTGGTGTGTTGACCGCCACCCAAGATACCTCCCTGCAAGATGGCTGTCTGATCTATAACGTGAAAATTCGTGGCGTCAACTTTACGAGCAATGGTCCGGTGATGCAGAAGAAAACCCTGGGTTGGGAGGCGTTTACGGAAACCCTGTATCCGGCCGATGGTGGCCTGGAGGGCCGTAACGACATGGCACTGAAGCTGGTTGGTGGCAGCCATTTGATCGCAAATATCAAGACGACGTACCGCAGCAAGAAACCGGCGAAAAATCTGAAGATGCCGGGTGTTTACTATGTCGACTACCGTCTGGAACGCATTAAAGAAGCGAATAATGAGACTTACGTGGAGCAGCACGAGGTTGCAGTCGCGCGCTATTGCGACTTGCCTAGCAAGCTGGGTCATAAACTGAAT" . + . + . + . + "negative control 2018 interlab" . + "R0040" . + . + . + "R0040" . + . + . + . + "R0040_sequence" . + "TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCAC" . + . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + . + . + . + "" . + "All_FPs" . + . + . + "All FPs" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "All_FPs_template" . + . + . + . + . + "" . + "B0015" . + . + . + "B0015" . + . + . + . + "B0015_sequence" . + "CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA" . + . + . + . + "Moderate (?)" . + "B0032" . + . + . + "B0032" . + . + . + . + "B0032_sequence" . + "TCACACAGGAAAG" . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "SubComponent3" . + . + . + . + "5' UTR including B0032 RBS and BB scars (long and short)" . + "BB_B0032_BB" . + . + . + . + . + . + . + "BB-B0032-BB" . + . + . + . + "Designs for round 1 if iGEM 2021 calibration experimentation" . + "BasicParts" . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + "Fluorescence calibration basic parts" . + . + "BioBricks scar including ag (not before CDS)" . + "BioBricks_scar_long" . + . + . + "BioBricks_scar_long" . + . + . + . + "BioBricks_scar_long_sequence" . + "TACTAGAG" . + . + . + . + "BioBricks scar (short one, w/o ag, before CDS)" . + "BioBricks_scar_short" . + . + . + "BioBricks_scar_short" . + . + . + . + "BioBricks_scar_short_sequence" . + "TACTAG" . + . + . + . + "Designs for round 1 if iGEM 2021 calibration experimentation" . + "CompositeParts" . + . + . + . + . + . + . + . + "Fluorescence calibration devices" . + . + "Cyan FP; codon optimized using IDT tool \nECFP" . + "ECFP" . + . + . + "ECFP" . + . + . + . + . + "ECFP_sequence" . + "ATGGTTAGTAAAGGGGAAGAGTTGTTTACGGGCGTTGTTCCAATCTTGGTGGAGTTGGACGGGGACGTTAATGGGCACAAATTTTCGGTCTCTGGGGAAGGCGAGGGGGACGCCACGTATGGTAAGCTGACCTTGAAGTTCATTTGCACTACTGGGAAATTGCCGGTTCCCTGGCCTACGCTTGTTACCACGTTGACCTGGGGCGTACAATGTTTCTCGCGCTATCCTGACCACATGAAACAACATGATTTCTTTAAGTCAGCTATGCCTGAGGGCTACGTGCAGGAGCGCACTATCTTCTTCAAGGATGACGGGAATTACAAAACCCGTGCGGAGGTAAAGTTCGAGGGTGACACGCTTGTCAACCGCATCGAGTTGAAGGGCATTGATTTTAAGGAGGATGGCAACATTTTAGGACATAAATTAGAGTATAACTATATTTCGCATAATGTCTACATTACTGCTGACAAACAAAAGAACGGCATCAAGGCTAATTTCAAAATTCGCCACAACATCGAGGATGGATCGGTTCAGTTAGCAGATCATTACCAGCAAAACACGCCCATCGGTGACGGACCAGTTCTGTTACCTGACAACCACTATCTTTCTACACAGAGTGCTCTTAGCAAGGACCCTAACGAAAAACGTGACCACATGGTCCTGCTGGAGTTTGTGACAGCCGCAGGTATTACATTGGGGATGGACGAATTGTATAAG" . + . + . + . + . + "VariableFeature1" . + . + . + . + "FPs_small" . + . + . + "FPs small" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + "VariableFeature2" . + . + . + . + "" . + "FPs_small_ins" . + . + . + . + "FPs small insert" . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "Constraint3" . + . + . + . + . + "Constraint4" . + . + . + . + . + "Constraint5" . + . + . + . + . + "LocalSubComponent1" . + "Part 2" . + . + . + . + "LocalSubComponent2" . + "Part 4" . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "SubComponent3" . + . + . + . + "SubComponent4" . + . + . + . + "FPs_small_ins_template" . + . + . + . + . + . + . + . + . + . + . + . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "FPs_small_template" . + . + . + . + . + . + . + . + "Final products desired for actual fabrication" . + "FinalProducts" . + . + . + . + "Final Products" . + . + "Linear DNA constructs to be fabricated" . + "LinearDNAProducts" . + . + . + . + "Linear DNA Products" . + . + . + "VariableFeature1" . + . + . + . + . + "" . + "Round_1_order" . + . + . + "Round 1 order" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "Round_1_order_template" . + . + . + . + . + "2 stop codons" . + "STOP" . + . + . + "STOP" . + . + . + . + "STOP_sequence" . + "TAATAA" . + . + . + . + "" . + "UNSX" . + . + . + "UNSX" . + . + . + . + "Constraint1" . + . + . + . + . + "SubComponent1" . + . + . + . + "SubComponent2" . + . + . + . + "" . + "UNSX_UP" . + . + . + . + . + "UNSX-UP" . + . + . + . + "UNSX_sequence" . + "CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC" . + . + . + . + "consensus without runs of As and Ts -59 to -36" . + "UP_element_consensus" . + . + . + "UP element consensus" . + . + . + . + "UP_element_consensus_sequence" . + "TGAAATATATTTTCGAAAAGCCGC" . + . + . + . + . + "VariableFeature1" . + . + . + . + "_2018_Interlab" . + . + . + "2018 Interlab" . + . + . + . + "VariableFeature1" . + . + . + . + . + . + . + . + . + "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . + "_2018_Interlab_ins" . + . + . + "2018 Interlab insert" . + . + . + . + "LocalSubComponent1" . + "Part 1" . + . + . + . + "_2018_Interlab_ins_template" . + . + . + . + . + "Constraint1" . + . + . + . + . + "Constraint2" . + . + . + . + . + "LocalSubComponent1" . + "Inserted Construct" . + . + . + "SubComponent1" . + . + . + "_2018_Interlab_template" . + . + . + . + . + . + . + . + "Blue FP codon optimized" . + "mTagBFP2" . + . + . + "mTagBFP2" . + . + . + . + "mTagBFP2_sequence" . + "ATGGTATCGAAGGGTGAAGAGTTGATTAAGGAAAACATGCACATGAAGTTATACATGGAAGGGACCGTGGATAATCACCATTTCAAATGCACATCTGAAGGAGAGGGAAAACCTTACGAAGGGACCCAGACAATGCGTATCAAAGTGGTCGAGGGTGGCCCTTTACCATTCGCGTTCGACATCCTGGCGACCTCCTTTTTATATGGGAGCAAAACATTCATTAACCACACCCAGGGAATCCCGGATTTCTTCAAGCAGTCTTTCCCTGAAGGGTTTACGTGGGAGCGTGTGACAACTTATGAGGATGGCGGGGTTTTGACGGCTACGCAGGATACTTCGTTACAAGACGGTTGCTTAATTTACAATGTAAAGATTCGTGGAGTAAATTTTACATCAAACGGTCCGGTCATGCAAAAGAAAACCCTTGGTTGGGAGGCGTTTACCGAGACATTGTATCCTGCCGACGGGGGATTAGAGGGTCGCAATGATATGGCACTGAAACTTGTGGGGGGGTCTCATTTAATTGCAAACGCTAAGACAACTTACCGCTCCAAGAAGCCCGCTAAGAACCTGAAAATGCCTGGGGTCTACTATGTTGACTATCGCCTTGAGCGTATCAAGGAGGCGAACAACGAGACTTACGTCGAGCAGCATGAGGTAGCGGTTGCCCGTTACTGTGACCTGCCATCTAAGTTAGGACATAAGTTGAAT" . + . + . + . + "Cyan FP (free); codon optimized using IDT tool \nmeffCFP" . + "meffCFP" . + . + . + "meffCFP" . + . + . + . + . + "meffCFP_sequence" . + "ATGGCGCTTTCAAAGCAGAGTTTACCGAGTGACATGAAGTTGATCTACCACATGGACGGGAACGTGAATGGTCACTCTTTCGTCATTAAGGGTGAAGGCGAGGGAAAACCCTATGAAGGAACACATACTATCAAGCTGCAAGTTGTTGAGGGCTCACCGTTACCTTTCAGCGCCGACATTCTGTCCACAGTTTTTCAATATGGGAACCGCTGTTTCACTAAGTATCCGCCTAATATCGTCGATTATTTTAAGAATAGCTGTTCTGGTGGGGGCTACAAGTTTGGACGCAGCTTTCTTTACGAAGACGGTGCGGTCTGTACGGCGTCAGGCGATATTACTCTGTCCGCAGACAAAAAGAGCTTTGAACACAAATCGAAATTCTTAGGCGTGAACTTCCCGGCTGACGGTCCGGTGATGAAGAAAGAGACCACCAACTGGGAACCATCGTGCGAAAAAATGACTCCGAATGGCATGACACTGATCGGTGACGTTACGGGTTTTTTGTTGAAGGAGGACGGAAAGCGTTACAAATGTCAATTTCATACCTTCCATGACGCTAAAGATAAGTCCAAAAAGATGCCGATGCCCGACTTCCACTTTGTCCAGCATAAAATTGAGCGCAAGGACCTTCCTGGAAGTATGCAAACATGGCGTCTTACCGAGCACGCGGCAGCATGTAAAACTTGTTTTACGGAG" . + . + . + . + "Blue FP (free); codon optimized using IDT tool \nmmilCFP" . + "mmilCFP" . + . + . + "mmilCFP" . + . + . + . + . + "mmilCFP_sequence" . + "ATGGCTCTGCCTAAACAAATGAAACTGACTTATCACATGGAGGGTACTGTGAACGGTCATTTTTTTATCATCAAGGGCGAAGGAGGGGGAGAGCCGTATGAGGGTACTCATACGATCAAGTTACAGGTGGTAGAGGGAAGCCCGTTGCCCTTTTCACCGGATATTCTTTCCACCGTCTTCCAATACGGCAACCGCTGCTTCACTAAATACCCTCCCAACATCGTTGATTACTTCAAAAACTCCTGCTCGGGCGGCGGTTATACTTTTGGACGTAGCTTCCTTTATGAGGACGGAGCTGTCTGTACTGCTTCCGGTGACATTACGCTGAGCAGCGACAAATCATCCTTTGAGCACAAATCTAAATTTCTGGGAGTAAACTTTCCTGCCGATGGCCCGGTGATGAAAAAAGAAACCACTAATTGGGAGCCTTCATGTGAGAAAATGACACCCAACGGGATGACCTTAATCGGTGATGTGACGGAGTTCCTGTTGAAGAAGGACGGTAAGCGTTACAAGTGCCAATTCCACACATTCCACGACGCGAAGGAGAAAAGCCGCAATATGCCGATGCCCGATTTCCATTTCGTTCAGCACGAGATTGAACGTAAAGATTTGCCAGGACCAATGCAAACATGGCAGTTGACCGAGCACGCAGCAGCCTGTAAGAACTGTTTCACAGAG" . + . + . + . + "pMB1 replicon, 100-300 copy" . + "pSB1C3" . + . + "pSB1C3" . + . + . + . + . + "" . + "LmrA" . + . + "LmrA" . + . + . + . + "Cyan FP; codon optimized using IDT tool \nmCerulean3" . + "ATP07149_1" . + . + . + "mCerulean3" . + . + . + . + . + "ATP07149_1_sequence" . + "ATGGTGTCGAAAGGTGAAGAACTTTTTACAGGTGTGGTCCCCATTTTAGTCGAATTAGATGGGGATGTGAATGGTCACAAATTCTCGGTGTCTGGGGAGGGTGAAGGTGACGCGACCTACGGGAAGTTGACTCTGAAATTCATTTGCACTACGGGGAAGTTACCAGTGCCCTGGCCCACTCTGGTTACAACACTGTCATGGGGCGTCCAATGTTTTGCTCGTTATCCTGACCACATGAAACAGCATGACTTCTTCAAGAGCGCAATGCCGGAAGGCTATGTCCAAGAGCGTACTATCTTTTTCAAAGACGATGGGAACTACAAAACACGCGCAGAAGTGAAGTTTGAGGGAGATACCCTGGTTAATCGCATTGAATTGAAAGGTATCGACTTCAAGGAGGATGGTAATATCCTGGGGCACAAATTGGAGTACAACGCTATCCACGGCAATGTTTACATTACGGCAGACAAACAGAAAAACGGAATTAAGGCCAATTTCGGACTTAACTGCAACATCGAAGATGGGTCGGTACAACTGGCAGACCACTATCAGCAAAATACGCCTATTGGTGACGGTCCTGTCCTGTTACCGGACAATCATTATCTTTCCACTCAGTCGAAACTGAGTAAAGATCCGAACGAGAAGCGCGATCACATGGTATTATTAGAGTTCGTCACAGCCGCAGGTATTACGTTAGGGATGGATGAGCTTTACAAG" . + . + . + . diff --git a/temp.py b/temp.py index 05b230c..5dca460 100644 --- a/temp.py +++ b/temp.py @@ -1,12 +1,17 @@ -import excel2sbol.converter as conv +# import excel2sbol.converter as conv +import sys import os +sys.path.insert(0, '/Users/william/Desktop/Code/GLL/Excel-to-SBOL/excel2sbol/excel2sbol') +import converter as conv + +cwd = os.chdir('/Users/william/Desktop/Code/GLL/Excel-utilities') cwd = os.getcwd() print(cwd) -file_path_in = os.path.join(cwd, 'SBOL3_simple_library4.xlsx') -file_path_out = os.path.join(cwd, 'SBOL3_simple_library4.nt') +file_path_in = os.path.join(cwd, 'two_backbones.xlsx') +file_path_out = os.path.join(cwd, 'two_backbones.nt') conv.converter(file_path_in, file_path_out, file_format='sorted nt') # conv.converter(file_path_in, file_path_out, sbol_version=2, diff --git a/temp3.py b/temp3.py index 7e6574a..97c32bf 100644 --- a/temp3.py +++ b/temp3.py @@ -24,7 +24,7 @@ def updateVariableFeatures(doc, combdev): if obj == None or type(obj) != sbol3.combderiv.CombinatorialDerivation: ins = False obj = doc.find(item) - obj.strategy = sbol3.SBOL_ENUMERATE + obj.strategy = sbol3.SBOL_ENUMERATE # Check This line if doc.find(f'{insert}_template'): template = doc.find(f'{insert}_template') @@ -37,13 +37,16 @@ def updateVariableFeatures(doc, combdev): # Go through and ensure that variantderivations are correct - removeList = [] - addList = [] + removeList = [] # List of variants to remove + addList = [] # List of variants to add as a subcomponent + + # combdev list of changed from combinatorial derivations to components for variant in variable_feature.variants: if str(variant) in combdev: addList.append(variant) removeList.append(variant) + # Check to see if correct reasoning but works for item in removeList: variable_feature.variants.remove(item) for item in addList: @@ -185,6 +188,7 @@ def updateVariableFeatures(doc, combdev): part += 1 # Clear the part # from the subcomponents + # Check this for feature in list(template.features): if type(feature) == sbol3.SubComponent: @@ -319,6 +323,7 @@ def convCombDeriv(file_path_in): tempcombdev[item] = dictionaryObj[item] # 1. Check to see if the object has an inserted construct + # Go through all combinatorial derivations - if has insert, remove insert from tempcombdev and add top level object to combdev for item in dictionaryObj: ins = item + "_ins" if ins in dictionaryObj: @@ -349,7 +354,7 @@ def convCombDeriv(file_path_in): combdev[item] = tempcombdev[item] break - + # Check if need to remove from tempcombdev after adding to combdev # 4. For every item in tempcombdev (Not a combinatorial derivation), convert it to a component @@ -404,10 +409,15 @@ def convCombDeriv(file_path_in): doc.add(newComponents[item]) # 7. Update the variable features in each of the combinatorial derivation objects if there are conversions + # Might have to update to make sure that it's the correct logic (If there are any changes to combinatorial derivations) if tempcombdev != combdev: updateVariableFeatures(doc, combdev) # 8. Update the uri references + # Not sure if this is necessary - doesn't seem to make a difference + # Take a new dictionary mapping the identity of the old objects to the newly created components + # Plug it into the helper function to update the uri refs (Not sure if does anything) + oldToNew = {} for item in tempcombdev: diff --git a/two_backbones.nt b/two_backbones.nt index ffcd86f..40ac87a 100644 --- a/two_backbones.nt +++ b/two_backbones.nt @@ -108,7 +108,7 @@ . . . - "Collection from the Basic Parts sheet" . + "Designs for round 1 if iGEM 2021 calibration experimentation" . "Basic_Parts" . . . @@ -118,17 +118,16 @@ . . . - . - . . - "Basic Parts" . + . + "Fluorescence calibration basic parts" . . - "Collection from the Composite Parts sheet" . + "Designs for round 1 if iGEM 2021 calibration experimentation" . "Composite_Parts" . . . . - "Composite Parts" . + "Fluorescence calibration devices" . . "Final products desired for actual fabrication" . "FinalProducts" . @@ -152,8 +151,8 @@ . "VariableFeature2" . . - . . + . . "Two_by_six" . . @@ -215,23 +214,17 @@ . . . - "pOpen_v4" . - . - "pOpen_v4" . - . - . - . - . "pMB1 replicon, 100-300 copy" . "pSB1C3" . . 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. + "Designs for round 1 if iGEM 2021 calibration experimentation" . "BasicParts" . . . @@ -120,17 +120,16 @@ . . . - . - . . - "Basic Parts" . + . + "Fluorescence calibration basic parts" . . - "Collection from the Composite Parts sheet" . + "Designs for round 1 if iGEM 2021 calibration experimentation" . "CompositeParts" . . . . - "Composite Parts" . + "Fluorescence calibration devices" . . "Final products desired for actual fabrication" . "FinalProducts" . @@ -154,8 +153,8 @@ . "VariableFeature2" . . - . . + . . "Two_by_six" . . @@ -218,24 +217,18 @@ . . . - "" . - "pOpen_v4" . - . - "pOpen_v4" . - . - . - . - . "pMB1 replicon, 100-300 copy" . "pSB1C3" . . "pSB1C3" . . - . . . - "testfiles" . - . - "" . - . - . + "" . + "BBF10K_000589" . + . + "pOpen_v4" . + . + . + . + . From b5c4c584febaef41513bdbdab16d175b29d76ef3 Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 7 May 2024 12:20:26 -0600 Subject: [PATCH 16/17] Worked on changing namespace for GenBank and FASTA --- excelutils/excel_sbol_utils/library2.py | 3 +-- excelutils/excel_sbol_utils/library3.py | 28 +++++++++++++++++++++++++ 2 files changed, 29 insertions(+), 2 deletions(-) diff --git a/excelutils/excel_sbol_utils/library2.py b/excelutils/excel_sbol_utils/library2.py index ade6b51..a6eee48 100644 --- a/excelutils/excel_sbol_utils/library2.py +++ b/excelutils/excel_sbol_utils/library2.py @@ -117,8 +117,7 @@ def subcomponents(rowobj): template.assemblePrimaryStructure(comp_list) #template.compile(assembly_method=None) - f#or comp in non_var_comps: - + #or comp in non_var_comps: rowobj.obj.masterTemplate = template for var in variant_comps: diff --git a/excelutils/excel_sbol_utils/library3.py b/excelutils/excel_sbol_utils/library3.py index 4ddf081..9234ff0 100644 --- a/excelutils/excel_sbol_utils/library3.py +++ b/excelutils/excel_sbol_utils/library3.py @@ -266,6 +266,34 @@ def dataSource(rowobj): rowobj.obj.update_all_dependents(id_map) # this function doesn't yet do everything it should rowobj.data_source_id_to_update[old_id] = new_identity + if pref == 'URL for GenBank file' or pref == 'URL for FASTA file': + # Namespace is everything except the last part of the url + # Loop backward through the value until a '/' is found + # Everything before the '/' is the namespace + old_val = val + + # Loop through the string backwards + for i in range(len(val) - 1, 0, -1): + if val[i] == '/': + # Everything before the '/' is the namespace + ns = val[:i] + + # Everything after the '/' is the display id + val = val[i+1:len(val) - 3] + + break + old_id = rowobj.obj.identity + rowobj.doc.change_object_namespace([rowobj.obj], ns) + new_id = rowobj.obj.identity + rowobj.data_source_id_to_update[old_id] = new_id + rowobj.obj.derived_from = [old_val] + if val != rowobj.obj.display_id: + new_identity = str(rowobj.obj.identity).replace(rowobj.obj.display_id, helpers.check_name(val)) + id_map = {rowobj.obj.identity:new_identity} + rowobj.obj.set_identity(new_identity) + rowobj.obj.update_all_dependents(id_map) # this function doesn't yet do everything it should + rowobj.data_source_id_to_update[old_id] = new_identity + def sequence(rowobj): for col in rowobj.col_cell_dict.keys(): val = rowobj.col_cell_dict[col] From 627d54372d0508b7a8bf64b35501ebeef6b4c87f Mon Sep 17 00:00:00 2001 From: Will Dravenstott Date: Tue, 7 May 2024 12:21:07 -0600 Subject: [PATCH 17/17] Example output for simple_library --- SampleTemp3Output.nt | 38 +++++++++++++++++++++++--------------- 1 file changed, 23 insertions(+), 15 deletions(-) diff --git a/SampleTemp3Output.nt b/SampleTemp3Output.nt index c19ceb2..4e31d60 100644 --- a/SampleTemp3Output.nt +++ b/SampleTemp3Output.nt @@ -167,10 +167,12 @@ . . . + "" . "All_FPs" . . . "All FPs" . + . . . "LocalSubComponent1" . @@ -183,6 +185,7 @@ . . . + "" . "B0015" . . . @@ -219,15 +222,15 @@ . . "SubComponent1" . - . + . . . "SubComponent2" . - . + . . . "SubComponent3" . - . + . . . "5' UTR including B0032 RBS and BB scars (long and short)" . @@ -304,10 +307,10 @@ . . . - . + . . . - . + . "Fluorescence calibration devices" . . "Cyan FP; codon optimized using IDT tool \nECFP" . @@ -344,8 +347,9 @@ . "VariableFeature2" . . - . + . . + "" . "FPs_small_ins" . . . @@ -382,12 +386,12 @@ "LocalSubComponent1" . "Part 2" . . - . + . . "LocalSubComponent2" . "Part 4" . . - . + . . "SubComponent1" . . @@ -418,7 +422,6 @@ . . . - "FPs_small_ins_template" . . . "Constraint1" . @@ -446,13 +449,14 @@ . . . + "Final products desired for actual fabrication" . "FinalProducts" . . . . "Final Products" . . - "" . + "Linear DNA constructs to be fabricated" . "LinearDNAProducts" . . . @@ -462,13 +466,15 @@ . "VariableFeature1" . . - . - . + . + . . + "" . "Round_1_order" . . . "Round 1 order" . + . . . "LocalSubComponent1" . @@ -494,6 +500,7 @@ . . . + "" . "UNSX" . . . @@ -514,6 +521,7 @@ . . . + "" . "UNSX_UP" . . . @@ -546,7 +554,6 @@ . . . - "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . "_2018_Interlab" . . . @@ -563,6 +570,7 @@ . . . + "Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length" . "_2018_Interlab_ins" . . . @@ -578,7 +586,6 @@ "_2018_Interlab_ins_template" . . . - "_2018_Interlab_ins_template" . . . "Constraint1" . @@ -653,11 +660,12 @@ "pSB1C3" . . . + . . + "" . "LmrA" . . "LmrA" . - . . . .