microscopy_club.md

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We are people who do microscopy or want to learn about it. Our club is very diverse: physicists, biologists, computer scientists, and DIY geeks. We meet monthly and talk about ray and wave optics, old and new microscopy methods, fluorescent probes, cameras, objectives, etc. Volunteers and guest speakers are welcome!

To subscribe, send an email to [email protected].

Once you are a member, post your messages to the mClub by writing an email to [email protected]

To unsubscribe, send an email to [email protected].

Add mClub to your Google calendar and stay updated!

Upcoming meetings:

TBA

Past meetings:

12.02.2020

**Inna Kviatovsky** ([Ramelow Lab, HU](https://www.physics.hu-berlin.de/en/niqo/people)) "Microscopy with *undetected* photons in the mid-IR"

12.12.2019

**Philipp Rauch** ([Lumicks](https://lumicks.com/)) "Real-time single-molecule investigation of dynamic biological processes using correlative optical tweezers and fluorescence microscopy: From structure to function."

27.11.2019

**Daniel von Wangenheim** ([3i](https://www.intelligent-imaging.com/)) "Advanced microscopy by 3i - Intelligent Imaging Innovations."

6.11.2019

**Maximilian Hoffmann** ([Judkewitz Lab](https://www.jlab.berlin/)) "Oblique plane microscopy for large field of views." Talk + DEMO of the brand new microscope!

30.10.2019

**Ella de Gaulejac** (Reber Lab) "Combining Expansion Microscopy and STED Microscopy: current tools towards super resolution in fluorescence microscopy”

Abin Biswas (Reber Lab) "Optical Diffraction Tomography: A quantitative label-free 3D imaging tool"

9.10.2019

**Simone Reber** "How Complexity Arises from Molecular Interactions"

Tobias Kletter (Reber Lab) “Teaching machines to do all the work: using adaptive feedback microscopy on complex samples”

15.05.2019

**Stephan Prebisch**: Simulating light propagation in tissues using raytracing.

Nikita Vladimirov: Highlights from Swiss Light-sheet Microscopy Workshop.

13.03.2019

**Martin Fritsch** and **Svenja Kunerth** (Leica Microsystems). Lightning and Thunder adaptive image extraction and deconvolution.

6.03.2019

**Marie Walde** (Woehler Lab). Confocal detectors.

Nikita Vladimirov (Preibisch Lab). Deconvolution for dummies.

30.01.2019

**Friedrich Preusser** (Preibisch Lab). FISHing under microscopes: An overview of recent techniques for spatial transcriptomics.

Journal club (F. Preusser): DNA microscopy: Optics-free spatio-genetic imaging by a stand-alone chemical reaction. J.A. Weinstein, A.Regev, F.Zhang.

Nikita Vladimirov (Preibisch Lab). Metrology hands-on demo: how to measure hair width and other tiny things with micron precision without a microscope.

5.12.2018

**Demo an ultra-compact microscope for confocal/SIM/TIRF/single molecule imaging**, [Nanoimager](https://oni.bio/).

15.11.2018

**Christopher Schmied**, [FMP](https://www.leibniz-fmp.de/core-facilities/cellular-imaging/wiesner/research.html). Digital images and their analysis.

11.10.2018

**Selma E. Anton**, [Lohse Lab](https://www.mdc-berlin.de/lohse). Lasers: where do the colors come from, what can they be used for and everything else you need to know.

Nikita Vladimirov, Preibisch Lab. Imaging, brain mapping, and messing with neuronal networks in zebrafish brain (journal club).

20.06.2018

**Elisabeth Baumann**, [Gerhardt Lab](https://www.mdc-berlin.de/gerhardt), MDC. Creating sound with light: The principle and applications of Photoacoustic Microscopy.

9.05.2018

**Marie Walde**, [Woehler Lab](https://www.mdc-berlin.de/light-microscopy), MDC. Co-localization analysis of fluorescence images: Overview & recent development. Journal Club: Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics, [Lagache et al](https://www.nature.com/articles/s41467-018-03053-x), Nat. Comm. 2018.

14.02.2018

**Konstantin Grohmann**, [Advanced Light Microscopy platform](https://www.mdc-berlin.de/9126126/en/research/core_facilities/Advanced-Light-Microscopy), MDC. Examining the performance of a fluorescence microscope. [Slides](/assets/mClub-Grohmann-2018Feb-Performance.zip){:target="_blank"}

24.01.2018

**Benjamin Judkewitz, head of the [Bioimaging and Neurophotonics Lab](https://www.jlab.berlin/) at Charité.** Deep imaging with time-reversed light.

06.12.2017

**An unresolved mystery of physics: the Double-Slit Experiment**. Watching [The Quantum Experiment that Broke Reality | Space Time | PBS Digital Studios](https://www.youtube.com/watch?v=p-MNSLsjjdo){:target="_blank"} and the classics, [Richard Feynman's Lecture on Electron Two-Slit Experiment](https://www.youtube.com/watch?v=2mIk3wBJDgE){:target="_blank"}

01.11.2017

**Sensors and cameras.** Discussion of **Nico Stuurman's** [online lectures](https://www.youtube.com/watch?v=8WpCov8iYCU){:target="_blank"} from [ibiology.org](https://www.ibiology.org/){:target="_blank"}

04.10.2017

**Martin Lehmann**, Core Facility Cellular Imaging FMP Introduction to fluorescent probes in microscopy. Quantitative assessment of fluorescent proteins. ([Cranfill et al., Nat. Methods, 2016](https://www.nature.com/nmeth/journal/v13/n7/full/nmeth.3891.html){:target="_blank"}). [Slides](/assets/mClub-Lehmann-2017Oct-Fluorescent_proteins.zip){:target="_blank"}

30.08.2017

**Nikita Vladimirov**, Preibisch Lab Autofocus in microscopy and photography. RAPID: Real-time image-based autofocus for all wide-field optical microscopy systems. ([Silvestri et al, 2017](https://www.biorxiv.org/content/early/2017/07/31/170555){:target="_blank"}). [Slides](/assets/mClub-Vladimirov-2017August-Autofocus.zip){:target="_blank"}

06.06.2017

**Varun Kapoor**, Preibisch Lab Maxwell equations. [Slides](/assets/mClub-Kapoor-2017June-Maxwell.zip){:target="_blank"} **Nikita Vladimirov**, Preibisch Lab Gaussian and Bessel beams. [Slides](/assets/mClub-Vladimirov-2017June2017-GaussianBeams.zip){:target="_blank"}

03.05.2017

**Marie Walde**, Woehler Lab Optical resolution and diffraction. [Slides](/assets/mClub-Walde-2017May-Resolution.zip){:target="_blank"}

05.04.2017

**Nikita Vladimirov**, Preibisch Lab Geometrical optics. [Slides](/assets/mClub-Vladimirov-2017April-GeometricalOptics.zip){:target="_blank"}