diff --git a/src/prefect/flows.py b/src/prefect/flows.py
index e378e0d00161..af9ae12b5f70 100644
--- a/src/prefect/flows.py
+++ b/src/prefect/flows.py
@@ -2116,6 +2116,48 @@ def _sanitize_and_load_flow(
             )
             func_def.returns = None
 
+    # Remove flow decorator callback hooks
+
+    def get_decorator_function_name(func_decorator):
+        """Retrieve decorator function name."""
+        func_decorator = (
+            func_decorator.func
+            if isinstance(func_decorator, ast.Call)
+            else func_decorator
+        )
+        return (
+            func_decorator.attr
+            if isinstance(func_decorator, ast.Attribute)
+            else func_decorator.id
+        )
+
+    if func_def.decorator_list:
+        # Keep only prefect flow decorator only
+        func_def.decorator_list = [
+            func_decorator
+            for func_decorator in func_def.decorator_list
+            if get_decorator_function_name(func_decorator) == "flow"
+        ]
+        exclude_keyword_args = (
+            "on_failure",
+            "on_completion",
+            "on_cancellation",
+            "on_crashed",
+            "on_running",
+        )
+
+        # Exclude callable type keyword arguments from flow decorator
+        for func_decorator in func_def.decorator_list:
+            if not hasattr(func_decorator, "keywords"):
+                continue
+
+            func_decorator.keywords = list(
+                filter(
+                    lambda keyword_arg: keyword_arg.arg not in exclude_keyword_args,
+                    func_decorator.keywords,
+                )
+            )
+
     # Attempt to compile the function without annotations and defaults that
     # can't be compiled
     try:
diff --git a/tests/test_flows.py b/tests/test_flows.py
index b68de0d96708..584771952720 100644
--- a/tests/test_flows.py
+++ b/tests/test_flows.py
@@ -5225,3 +5225,30 @@ def dog():
 
         with pytest.raises(NameError, match="name 'not_a_function' is not defined"):
             safe_load_flow_from_entrypoint(entrypoint)()
+
+    def test_remove_callback_hooks_for_missing_import(self, tmp_path: Path):
+        flow_source = dedent(
+            """
+        from prefect import flow
+
+        from non_existent import DEFAULT_NAME, DEFAULT_AGE
+
+        def test_callback():
+            print(DEFAULT_NAME)
+            def wrapper(*args, **kwargs):
+                pass
+
+            return wrapper
+
+        @flow(on_running=[test_callback()])
+        def flow_function(name = DEFAULT_NAME, age = DEFAULT_AGE) -> str:
+            return name, age
+        """
+        )
+
+        tmp_path.joinpath("flow.py").write_text(flow_source)
+
+        entrypoint = f"{tmp_path.joinpath('flow.py')}:flow_function"
+
+        result = safe_load_flow_from_entrypoint(entrypoint)
+        assert result is not None