diff --git a/src/pynwb/__init__.py b/src/pynwb/__init__.py
index e8294057f..2eef7bbe9 100644
--- a/src/pynwb/__init__.py
+++ b/src/pynwb/__init__.py
@@ -514,11 +514,21 @@ def read_nwb(**kwargs):
         path = popargs('path', kwargs)
         file = popargs('file', kwargs)
 
-        # open the file with NWBZarrIO and rad the file
-        io = NWBHDF5IO(path=path, file=file, mode="r", load_namespaces=True)
-        nwbfile = io.read()
+        # Streaming case
+        if path is not None and str(path).startswith("s3://") or str(path).startswith("http"):
+            import fsspec
+            print(path)
+            path = "https://dandiarchive.s3.amazonaws.com/blobs/11e/c89/11ec8933-1456-4942-922b-94e5878bb991"
+            fsspec_file_system = fsspec.filesystem("http")
+            ffspec_file = fsspec_file_system.open(str(path), "rb")
+
+            open_file = h5py.File(ffspec_file, "r")
+            io = NWBHDF5IO(file=open_file)
+            nwbfile = io.read()
+        else:
+            io = NWBHDF5IO(path=path, file=file, mode="r", load_namespaces=True)
+            nwbfile = io.read()
 
-        # return the NWBFile object
         return nwbfile
 
 from . import io as __io  # noqa: F401,E402
diff --git a/tests/integration/hdf5/test_io.py b/tests/integration/hdf5/test_io.py
index d54201b7b..76921d290 100644
--- a/tests/integration/hdf5/test_io.py
+++ b/tests/integration/hdf5/test_io.py
@@ -4,6 +4,7 @@
 from h5py import File
 from pathlib import Path
 import tempfile
+import pytest
 
 from pynwb import NWBFile, TimeSeries, get_manager, NWBHDF5IO, validate
 
@@ -596,3 +597,12 @@ def test_read_nwb_method_file(self):
         self.assertContainerEqual(read_nwbfile, self.nwbfile)
 
         read_nwbfile.get_read_io().close()
+    
+    @pytest.mark.skipif(not pytest.importorskip("fsspec"), reason="fsspec library not available")
+    def test_read_nwb_method_s3_path(self):
+        
+        s3_test_path = "https://dandiarchive.s3.amazonaws.com/blobs/11e/c89/11ec8933-1456-4942-922b-94e5878bb991"
+        read_nwbfile = NWBHDF5IO.read_nwb(path=s3_test_path)
+        assert read_nwbfile.identifier == "3f77c586-6139-4777-a05d-f603e90b1330"
+    
+        assert read_nwbfile.subject.subject_id == "1"
\ No newline at end of file