diff --git a/tox.ini b/tox.ini
index f7e974745..798da6fe6 100644
--- a/tox.ini
+++ b/tox.ini
@@ -33,6 +33,15 @@ deps =
     -rrequirements-dev.txt
 commands = {[testenv]commands}
 
+# Test with python 3.12; pinned dev reqs; upgraded, pre-release run reqs
+[testenv:py312-prerelease]
+basepython = python3.12
+install_command =
+    python -m pip install -U --pre {opts} {packages}
+deps =
+    -rrequirements-dev.txt
+commands = {[testenv]commands}
+
 # Test with python 3.8; pinned dev reqs; minimum run reqs
 [testenv:py38-minimum]
 basepython = python3.8
@@ -75,6 +84,14 @@ deps =
     -rrequirements-dev.txt
 commands = {[testenv:build]commands}
 
+[testenv:build-py312-prerelease]
+basepython = python3.12
+install_command =
+    python -m pip install -U --pre {opts} {packages}
+deps =
+    -rrequirements-dev.txt
+commands = {[testenv:build]commands}
+
 [testenv:build-py38-minimum]
 basepython = python3.8
 deps =
@@ -88,7 +105,7 @@ deps = null
 commands = python -c "import pynwb"
 
 # Envs that will execute gallery tests that do not require ROS3
-# Test with pinned dev, doc, run, and optional reqs
+# Test with pinned dev, doc, and run reqs
 [testenv:gallery]
 install_command =
     python -m pip install -U {opts} {packages}
@@ -121,7 +138,7 @@ basepython = python3.11
 deps = {[testenv:gallery]deps}
 commands = {[testenv:gallery]commands}
 
-# Test with python 3.12; pinned dev, doc, and optional reqs; upgraded run reqs
+# Test with python 3.12; pinned dev, and doc reqs; upgraded run reqs
 [testenv:gallery-py312-upgraded]
 basepython = python3.12
 deps =