diff --git a/src/pynwb/testing/mock/file.py b/src/pynwb/testing/mock/file.py
index ee7fc5655..50369e7e1 100644
--- a/src/pynwb/testing/mock/file.py
+++ b/src/pynwb/testing/mock/file.py
@@ -1,7 +1,6 @@
 from typing import Optional
 from uuid import uuid4
 from datetime import datetime
-from dateutil.tz import tzlocal
 
 from ...file import NWBFile, Subject
 from .utils import name_generator
diff --git a/src/pynwb/testing/testh5io.py b/src/pynwb/testing/testh5io.py
index 6a5c96547..45ae8cebe 100644
--- a/src/pynwb/testing/testh5io.py
+++ b/src/pynwb/testing/testh5io.py
@@ -1,5 +1,4 @@
 from datetime import datetime
-from dateutil.tz import tzlocal, tzutc
 import os
 from abc import ABCMeta, abstractmethod
 import warnings
diff --git a/tests/integration/hdf5/test_base.py b/tests/integration/hdf5/test_base.py
index 145f55d0f..75c346012 100644
--- a/tests/integration/hdf5/test_base.py
+++ b/tests/integration/hdf5/test_base.py
@@ -1,6 +1,5 @@
 import numpy as np
 from datetime import datetime
-from dateutil.tz import tzlocal
 
 from pynwb import TimeSeries, NWBFile, NWBHDF5IO
 from pynwb.base import Images, Image, ImageReferences
diff --git a/tests/integration/hdf5/test_modular_storage.py b/tests/integration/hdf5/test_modular_storage.py
index bfe71b772..09c916bfb 100644
--- a/tests/integration/hdf5/test_modular_storage.py
+++ b/tests/integration/hdf5/test_modular_storage.py
@@ -1,7 +1,6 @@
 import os
 import gc
 from datetime import datetime
-from dateutil.tz import tzutc
 import numpy as np
 
 from hdmf.backends.hdf5 import HDF5IO
diff --git a/tests/unit/test_epoch.py b/tests/unit/test_epoch.py
index 6cd3f6575..4bf2637df 100644
--- a/tests/unit/test_epoch.py
+++ b/tests/unit/test_epoch.py
@@ -1,7 +1,6 @@
 import numpy as np
 import pandas as pd
 from datetime import datetime
-from dateutil import tz
 
 from pynwb.epoch import TimeIntervals
 from pynwb import TimeSeries, NWBFile
diff --git a/tests/unit/test_extension.py b/tests/unit/test_extension.py
index a94268e93..abbb6511a 100644
--- a/tests/unit/test_extension.py
+++ b/tests/unit/test_extension.py
@@ -2,7 +2,6 @@
 import random
 import string
 from datetime import datetime
-from dateutil.tz import tzlocal
 from tempfile import gettempdir
 
 from hdmf.spec import RefSpec
diff --git a/tests/unit/test_icephys.py b/tests/unit/test_icephys.py
index 860221418..8ff1a67ba 100644
--- a/tests/unit/test_icephys.py
+++ b/tests/unit/test_icephys.py
@@ -14,7 +14,6 @@
 from pynwb.testing import TestCase
 from pynwb.file import NWBFile  # Needed to test icephys functionality defined on NWBFile
 from datetime import datetime
-from dateutil.tz import tzlocal
 
 
 def GetElectrode():
diff --git a/tests/unit/test_scratch.py b/tests/unit/test_scratch.py
index 29ecddbcc..c04a00e99 100644
--- a/tests/unit/test_scratch.py
+++ b/tests/unit/test_scratch.py
@@ -1,5 +1,4 @@
 from datetime import datetime
-from dateutil.tz import tzlocal
 import numpy as np
 from numpy.testing import assert_array_equal
 import pandas as pd