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Index error on tepid-discover #24

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LeoVincenzi opened this issue Sep 16, 2024 · 0 comments
Open

Index error on tepid-discover #24

LeoVincenzi opened this issue Sep 16, 2024 · 0 comments

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@LeoVincenzi
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Dear developers,
I'm having a problem running tepid-discovery. the mapping phase ends without any error, so I assume the bam files are okay.
Anyway, running the tepid-discovery step, I get the following error:

Processing Lupinus_digitatus
Running paired-end mode
Estimating mean insert size and coverage
Traceback (most recent call last):
  File "/home/leonardo/miniconda3/envs/TEPID/bin/tepid-discover", line 44, in <module>
    tepid.discover_pe(options)
  File "/home/leonardo/miniconda3/envs/TEPID/lib/python2.7/site-packages/tepid/tepid.py", line 1009, in discover_pe
    cov = calc_cov(options.conc, 100000, 120000)
  File "/home/leonardo/miniconda3/envs/TEPID/lib/python2.7/site-packages/tepid/tepid.py", line 721, in calc_cov
    for read in bam.pileup(nms[0], start, stop):
IndexError: list index out of range

The command line was:
tepid-discover --name Lupinus_digitatus --conc Lupinus_digitatus.sorted.bam --split Lupinus_digitatus.split.bam --te repeat.bed -p 30.
I also tried to run again the mapping step, but the problem remains.

Thanks in advance!

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