From 233aeab651cc68e6730e86b11a7e01a9ddd20934 Mon Sep 17 00:00:00 2001
From: lcolladotor Date the vignette was generated. Wallclock time spent generating the vignette. Date the vignette was generated. Wallclock time spent generating the vignette. Date the vignette was generated. Wallclock time spent generating the vignette. Once you have downloaded the objects, we can explore them a little
bit Date the vignette was generated. Wallclock time spent generating the vignette. BUG FIXES NEW FEATURES A From Gencode## Show the GTF cache location
gtf_cache
#> BFC4
-#> "/github/home/.cache/R/BiocFileCache/5c6a7dbe81_gencode.v32.annotation.gtf.gz"
+#> "/github/home/.cache/R/BiocFileCache/5c71d57fde_gencode.v32.annotation.gtf.gz"
## Import into R (takes ~1 min)
gtf <- rtracklayer::import(gtf_cache)
@@ -818,11 +818,11 @@
Wrapper functions= c("lowres", "hires", "detected", "aligned"), load = TRUE,
reference_gtf = gtf_cache
)
-#> 2024-12-12 20:13:56.419903 SpatialExperiment::read10xVisium: reading basic data from SpaceRanger
-#> 2024-12-12 20:14:06.59609 read10xVisiumAnalysis: reading analysis output from SpaceRanger
-#> 2024-12-12 20:14:07.647298 add10xVisiumAnalysis: adding analysis output from SpaceRanger
-#> 2024-12-12 20:14:07.801939 rtracklayer::import: reading the reference GTF file
-#> 2024-12-12 20:14:32.428294 adding gene information to the SPE object
+#> 2024-12-12 20:19:09.681751 SpatialExperiment::read10xVisium: reading basic data from SpaceRanger
+#> 2024-12-12 20:19:19.963575 read10xVisiumAnalysis: reading analysis output from SpaceRanger
+#> 2024-12-12 20:19:21.059095 add10xVisiumAnalysis: adding analysis output from SpaceRanger
+#> 2024-12-12 20:19:21.207331 rtracklayer::import: reading the reference GTF file
+#> 2024-12-12 20:19:45.832527 adding gene information to the SPE object
#> Warning: Gene IDs did not match. This typically happens when you are not using
#> the same GTF file as the one that was used by SpaceRanger. For example, one
#> file uses GENCODE IDs and the other one ENSEMBL IDs. read10xVisiumWrapper()
@@ -830,7 +830,7 @@
Wrapper functions#> Warning: Dropping 29 out of 36601 genes for which we don't have information on
#> the reference GTF file. This typically happens when you are not using the same
#> GTF file as the one that was used by SpaceRanger.
-#> 2024-12-12 20:14:33.863742 adding information used by spatialLIBD
+#> 2024-12-12 20:19:47.339986 adding information used by spatialLIBD
## Run our shiny app
if (interactive()) {
@@ -1114,9 +1114,9 @@
Reproducibilitylibrary("knitr")
knit("TenX_data_download.Rmd", tangle = TRUE)
+#> [1] "2024-12-12 20:14:34 UTC"
#> [1] "2024-12-12 20:19:48 UTC"
+#> Time difference of 1.646 mins
#> Time difference of 1.664 mins
R
session information.#> ─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────
#> setting value
@@ -1287,7 +1287,7 @@
Reproducibility#> SparseArray 1.6.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
#> sparseMatrixStats 1.18.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
#> SpatialExperiment * 1.16.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
-#> spatialLIBD * 1.19.1 2024-12-12 [1] Bioconductor
+#> spatialLIBD * 1.19.2 2024-12-12 [1] Bioconductor
#> statmod 1.5.0 2023-01-06 [1] RSPM (R 4.4.0)
#> stringi 1.8.4 2024-05-06 [2] RSPM (R 4.4.0)
#> stringr 1.5.1 2023-11-14 [2] RSPM (R 4.4.0)
diff --git a/articles/guide_to_spatial_registration.html b/articles/guide_to_spatial_registration.html
index 038e26f2..1c8e6ed5 100644
--- a/articles/guide_to_spatial_registration.html
+++ b/articles/guide_to_spatial_registration.html
@@ -39,7 +39,7 @@
@@ -288,7 +288,7 @@
Spatial Reference
+#> 2024-12-12 20:16:22.448405 make pseudobulk object
+#> 2024-12-12 20:16:24.149791 dropping 13 pseudo-bulked samples that are below 'min_ncells'.
+#> 2024-12-12 20:16:24.194799 drop lowly expressed genes
+#> 2024-12-12 20:16:24.349038 normalize expression
+#> 2024-12-12 20:16:25.890173 create model matrix
+#> 2024-12-12 20:16:25.913575 run duplicateCorrelation()
+#> 2024-12-12 20:16:38.936856 The estimated correlation is: 0.138734774807097
+#> 2024-12-12 20:16:38.939658 computing enrichment statistics
+#> 2024-12-12 20:16:40.187687 extract and reformat enrichment results
+#> 2024-12-12 20:16:40.245241 running the baseline pairwise model
+#> 2024-12-12 20:16:40.39009 computing pairwise statistics
+#> 2024-12-12 20:16:42.593259 computing F-statistics## get reference layer enrichment statistics
layer_modeling_results <- fetch_data(type = "modeling_results")
-#> 2024-12-12 20:11:00.268595 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:16:14.237373 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
layer_modeling_results$enrichment[1:5, 1:5]
#> t_stat_WM t_stat_Layer1 t_stat_Layer2 t_stat_Layer3 t_stat_Layer4
@@ -391,18 +391,18 @@
Get Enrichment statistics
gene_ensembl = "gene_id",
gene_name = "gene_name"
)
-#> 2024-12-12 20:11:08.137818 make pseudobulk object
-#> 2024-12-12 20:11:09.784836 dropping 13 pseudo-bulked samples that are below 'min_ncells'.
-#> 2024-12-12 20:11:09.829241 drop lowly expressed genes
-#> 2024-12-12 20:11:09.986814 normalize expression
-#> 2024-12-12 20:11:11.493804 create model matrix
-#> 2024-12-12 20:11:11.517093 run duplicateCorrelation()
-#> 2024-12-12 20:11:24.698387 The estimated correlation is: 0.138734774807097
-#> 2024-12-12 20:11:24.7013 computing enrichment statistics
-#> 2024-12-12 20:11:25.915521 extract and reformat enrichment results
-#> 2024-12-12 20:11:25.971312 running the baseline pairwise model
-#> 2024-12-12 20:11:26.125053 computing pairwise statistics
-#> 2024-12-12 20:11:28.250693 computing F-statistics
Extract Enrichment t-statistics
@@ -576,9 +576,9 @@
Reproducibilitylibrary("knitr")
knit("guide_to_spatial_registration.Rmd", tangle = TRUE)
+#> [1] "2024-12-12 20:11:31 UTC"
#> [1] "2024-12-12 20:16:45 UTC"
+#> Time difference of 43.561 secs
#> Time difference of 43.786 secs
R
session information.
diff --git a/reference/get_colors.html b/reference/get_colors.html
index daea5c30..d7960fe1 100644
--- a/reference/get_colors.html
+++ b/reference/get_colors.html
@@ -18,7 +18,7 @@
@@ -105,7 +105,7 @@ #> ─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────
#> setting value
@@ -737,7 +737,7 @@
Reproducibility#> spam 2.11-0 2024-10-03 [1] RSPM (R 4.4.0)
#> SparseArray 1.6.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
#> SpatialExperiment * 1.16.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
-#> spatialLIBD * 1.19.1 2024-12-12 [1] Bioconductor
+#> spatialLIBD * 1.19.2 2024-12-12 [1] Bioconductor
#> statmod 1.5.0 2023-01-06 [1] RSPM (R 4.4.0)
#> stringi 1.8.4 2024-05-06 [2] RSPM (R 4.4.0)
#> stringr 1.5.1 2023-11-14 [2] RSPM (R 4.4.0)
diff --git a/articles/index.html b/articles/index.html
index 6619dc98..135201eb 100644
--- a/articles/index.html
+++ b/articles/index.html
@@ -17,7 +17,7 @@
diff --git a/articles/multi_gene_plots.html b/articles/multi_gene_plots.html
index 22bfbb3c..fb27358f 100644
--- a/articles/multi_gene_plots.html
+++ b/articles/multi_gene_plots.html
@@ -39,7 +39,7 @@
@@ -195,9 +195,9 @@
Plotting One Gene
modeling_results <- fetch_data(type = "modeling_results")
-#> 2024-12-12 20:11:55.351142 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:17:08.833029 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
sce_layer <- fetch_data(type = "sce_layer")
-#> 2024-12-12 20:11:56.728664 loading file /github/home/.cache/R/BiocFileCache/5c61202c143_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
+#> 2024-12-12 20:17:09.724514 loading file /github/home/.cache/R/BiocFileCache/5c77b6ee4a2_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
sig_genes <- sig_genes_extract_all(
n = 400,
modeling_results = modeling_results,
@@ -421,9 +421,9 @@
Reproducibilitylibrary("knitr")
knit("multi_gene_plots.Rmd", tangle = TRUE)
+#> [1] "2024-12-12 20:12:12 UTC"
#> [1] "2024-12-12 20:17:25 UTC"
+#> Time difference of 37.141 secs
#> Time difference of 36.102 secs
R
session information.#> ─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────
#> setting value
@@ -581,7 +581,7 @@
Reproducibility#> spam 2.11-0 2024-10-03 [1] RSPM (R 4.4.0)
#> SparseArray 1.6.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
#> SpatialExperiment * 1.16.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
-#> spatialLIBD * 1.19.1 2024-12-12 [1] Bioconductor
+#> spatialLIBD * 1.19.2 2024-12-12 [1] Bioconductor
#> statmod 1.5.0 2023-01-06 [1] RSPM (R 4.4.0)
#> stringi 1.8.4 2024-05-06 [2] RSPM (R 4.4.0)
#> stringr 1.5.1 2023-11-14 [2] RSPM (R 4.4.0)
diff --git a/articles/spatialLIBD.html b/articles/spatialLIBD.html
index 2750a815..e22b6926 100644
--- a/articles/spatialLIBD.html
+++ b/articles/spatialLIBD.html
@@ -39,7 +39,7 @@
@@ -414,7 +414,7 @@
Downloading the data with
s
## Download the small example sce data
sce <- fetch_data(type = "sce_example", eh = ehub)
-#> 2024-12-12 20:12:28.826377 loading file /github/home/.cache/R/BiocFileCache/5c66f42346d_sce_sub_for_vignette.Rdata%3Fdl%3D1
+#> 2024-12-12 20:17:41.230595 loading file /github/home/.cache/R/BiocFileCache/5c772064dd8_sce_sub_for_vignette.Rdata%3Fdl%3D1
## Convert to a SpatialExperiment object
spe <- sce_to_spe(sce)
@@ -426,9 +426,9 @@
Downloading the data with
s
## Query ExperimentHub and download the data
if (!exists("sce_layer")) sce_layer <- fetch_data(type = "sce_layer", eh = ehub)
-#> 2024-12-12 20:12:33.031734 loading file /github/home/.cache/R/BiocFileCache/5c61202c143_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
+#> 2024-12-12 20:17:45.41416 loading file /github/home/.cache/R/BiocFileCache/5c77b6ee4a2_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
modeling_results <- fetch_data("modeling_results", eh = ehub)
-#> 2024-12-12 20:12:33.472108 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
@@ -501,7 +501,7 @@
Downloading the data with
s
)
)
#> user system elapsed
-#> 9.464 0.376 9.859
+#> 9.493 0.440 9.952
## Explore the result
class(sig_genes)
@@ -1383,9 +1383,9 @@
Reproducibilitylibrary("knitr")
knit("spatialLIBD.Rmd", tangle = TRUE)
+#> [1] "2024-12-12 20:12:51 UTC"
#> [1] "2024-12-12 20:18:04 UTC"
+#> Time difference of 35.593 secs
#> Time difference of 35.251 secs
R
session information.
diff --git a/reference/cluster_import.html b/reference/cluster_import.html
index 42881050..3bd53ea2 100644
--- a/reference/cluster_import.html
+++ b/reference/cluster_import.html
@@ -20,7 +20,7 @@
@@ -137,11 +137,11 @@ #> ─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────
#> setting value
@@ -1544,7 +1544,7 @@
Reproducibility#> spam 2.11-0 2024-10-03 [1] RSPM (R 4.4.0)
#> SparseArray 1.6.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
#> SpatialExperiment * 1.16.0 2024-10-29 [1] Bioconductor 3.20 (R 4.4.2)
-#> spatialLIBD * 1.19.1 2024-12-12 [1] Bioconductor
+#> spatialLIBD * 1.19.2 2024-12-12 [1] Bioconductor
#> statmod 1.5.0 2023-01-06 [1] RSPM (R 4.4.0)
#> stringi 1.8.4 2024-05-06 [2] RSPM (R 4.4.0)
#> stringr 1.5.1 2023-11-14 [2] RSPM (R 4.4.0)
diff --git a/authors.html b/authors.html
index b652a12d..a82f81ea 100644
--- a/authors.html
+++ b/authors.html
@@ -17,7 +17,7 @@
diff --git a/index.html b/index.html
index cbe5e0cd..35f54314 100644
--- a/index.html
+++ b/index.html
@@ -41,7 +41,7 @@
diff --git a/news/index.html b/news/index.html
index 20d6c300..0c83d694 100644
--- a/news/index.html
+++ b/news/index.html
@@ -17,7 +17,7 @@
@@ -66,6 +66,11 @@
Changelog
Source: NEWS.md
+ spatialLIBD 1.19.2
+registration_pseudobulk()
more robust.spatialLIBD 1.19.1
Examples
image_paths = c("151507" = "https://spatial-dlpfc.s3.us-east-2.amazonaws.com/images/151507_tissue_lowres_image.png")
))
}
-#> 2024-12-12 20:06:30.875077 loading file /github/home/.cache/R/BiocFileCache/1048142167f7_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
-#> 2024-12-12 20:06:41.543143 adding image for sample 151507
-#> 2024-12-12 20:06:42.863058 adding image for sample 151508
+#> 2024-12-12 20:12:01.22569 loading file /github/home/.cache/R/BiocFileCache/104970f7ab1e_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:12.020288 adding image for sample 151507
+#> 2024-12-12 20:12:12.591118 adding image for sample 151508
#> Warning: No image was found for sample: 151508
-#> 2024-12-12 20:06:42.863794 adding image for sample 151509
+#> 2024-12-12 20:12:12.591867 adding image for sample 151509
#> Warning: No image was found for sample: 151509
-#> 2024-12-12 20:06:42.864312 adding image for sample 151510
+#> 2024-12-12 20:12:12.592384 adding image for sample 151510
#> Warning: No image was found for sample: 151510
-#> 2024-12-12 20:06:42.864798 adding image for sample 151669
+#> 2024-12-12 20:12:12.592872 adding image for sample 151669
#> Warning: No image was found for sample: 151669
-#> 2024-12-12 20:06:42.865274 adding image for sample 151670
+#> 2024-12-12 20:12:12.593351 adding image for sample 151670
#> Warning: No image was found for sample: 151670
-#> 2024-12-12 20:06:42.865771 adding image for sample 151671
+#> 2024-12-12 20:12:12.593823 adding image for sample 151671
#> Warning: No image was found for sample: 151671
-#> 2024-12-12 20:06:42.86624 adding image for sample 151672
+#> 2024-12-12 20:12:12.594292 adding image for sample 151672
#> Warning: No image was found for sample: 151672
-#> 2024-12-12 20:06:42.866706 adding image for sample 151673
+#> 2024-12-12 20:12:12.594766 adding image for sample 151673
#> Warning: No image was found for sample: 151673
-#> 2024-12-12 20:06:42.867163 adding image for sample 151674
+#> 2024-12-12 20:12:12.59522 adding image for sample 151674
#> Warning: No image was found for sample: 151674
-#> 2024-12-12 20:06:42.867632 adding image for sample 151675
+#> 2024-12-12 20:12:12.595699 adding image for sample 151675
#> Warning: No image was found for sample: 151675
-#> 2024-12-12 20:06:42.868106 adding image for sample 151676
+#> 2024-12-12 20:12:12.596159 adding image for sample 151676
#> Warning: No image was found for sample: 151676
#> DataFrame with 13 rows and 4 columns
#> sample_id image_id data scaleFactor
diff --git a/reference/add_key.html b/reference/add_key.html
index 0dc639cd..4899b513 100644
--- a/reference/add_key.html
+++ b/reference/add_key.html
@@ -19,7 +19,7 @@
@@ -124,7 +124,7 @@ Examples
spe$key_original <- NULL
head(spe$key)
}
-#> 2024-12-12 20:06:45.589277 loading file /github/home/.cache/R/BiocFileCache/1048142167f7_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:14.88532 loading file /github/home/.cache/R/BiocFileCache/104970f7ab1e_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
#> [1] "151507_AAACAACGAATAGTTC-1" "151507_AAACAAGTATCTCCCA-1"
#> [3] "151507_AAACAATCTACTAGCA-1" "151507_AAACACCAATAACTGC-1"
#> [5] "151507_AAACAGCTTTCAGAAG-1" "151507_AAACAGGGTCTATATT-1"
diff --git a/reference/add_qc_metrics.html b/reference/add_qc_metrics.html
index 63d9579f..1889e071 100644
--- a/reference/add_qc_metrics.html
+++ b/reference/add_qc_metrics.html
@@ -21,7 +21,7 @@
@@ -144,7 +144,7 @@ Author
Examples
## Obtain the necessary data
spe_pre_qc <- fetch_data("spatialDLPFC_Visium_example_subset")
-#> 2024-12-12 20:06:56.981917 loading file /github/home/.cache/R/BiocFileCache/5c64699e3d1_spatialDLPFC_spe_subset_example.rds%3Fdl%3D1
+#> 2024-12-12 20:12:25.69285 loading file /github/home/.cache/R/BiocFileCache/5c73918b4f0_spatialDLPFC_spe_subset_example.rds%3Fdl%3D1
## For now, we fake out tissue spots in example data
spe_qc <- spe_pre_qc
diff --git a/reference/annotate_registered_clusters.html b/reference/annotate_registered_clusters.html
index 51e42c5b..55c45daa 100644
--- a/reference/annotate_registered_clusters.html
+++ b/reference/annotate_registered_clusters.html
@@ -21,7 +21,7 @@
Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
-#> 2024-12-12 20:07:09.146699 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:37.236166 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
## Compute the correlations
cor_stats_layer <- layer_stat_cor(
diff --git a/reference/check_modeling_results.html b/reference/check_modeling_results.html
index edd4bd31..1728a83c 100644
--- a/reference/check_modeling_results.html
+++ b/reference/check_modeling_results.html
@@ -18,7 +18,7 @@
@@ -109,7 +109,7 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
-#> 2024-12-12 20:07:10.459215 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:38.247754 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
## Check the object
xx <- check_modeling_results(modeling_results)
diff --git a/reference/check_sce.html b/reference/check_sce.html
index 5709551d..007aeca7 100644
--- a/reference/check_sce.html
+++ b/reference/check_sce.html
@@ -20,7 +20,7 @@
@@ -131,7 +131,7 @@ Examples
## Check the object
check_sce(sce_example)
}
-#> 2024-12-12 20:07:11.726206 loading file /github/home/.cache/R/BiocFileCache/5c66f42346d_sce_sub_for_vignette.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:39.193695 loading file /github/home/.cache/R/BiocFileCache/5c772064dd8_sce_sub_for_vignette.Rdata%3Fdl%3D1
#> class: SingleCellExperiment
#> dim: 33538 47681
#> metadata(1): image
diff --git a/reference/check_sce_layer.html b/reference/check_sce_layer.html
index 75a4aa27..1a3b8e32 100644
--- a/reference/check_sce_layer.html
+++ b/reference/check_sce_layer.html
@@ -18,7 +18,7 @@
@@ -112,7 +112,7 @@ Examples
## Obtain example data from the HumanPilot project
## (Maynard, Collado-Torres, et al, 2021)
if (!exists("sce_layer")) sce_layer <- fetch_data("sce_layer")
-#> 2024-12-12 20:07:14.244422 loading file /github/home/.cache/R/BiocFileCache/5c61202c143_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
+#> 2024-12-12 20:12:41.34337 loading file /github/home/.cache/R/BiocFileCache/5c77b6ee4a2_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
## Check the pseudo-bulked data
check_sce_layer(sce_layer)
diff --git a/reference/check_spe.html b/reference/check_spe.html
index 6241cb53..d67cc3f5 100644
--- a/reference/check_spe.html
+++ b/reference/check_spe.html
@@ -18,7 +18,7 @@
@@ -123,7 +123,7 @@ Examples
## Check the object
check_spe(spe)
}
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#> class: SpatialExperiment
#> dim: 33538 47681
#> metadata(0):
diff --git a/reference/cluster_export.html b/reference/cluster_export.html
index a9699fb4..3844caf5 100644
--- a/reference/cluster_export.html
+++ b/reference/cluster_export.html
@@ -21,7 +21,7 @@
@@ -134,7 +134,7 @@ Examples
cluster_export(spe, "spatialLIBD")
cluster_export(spe, "GraphBased")
}
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#> Overwriting 'spe$key'. Set 'overwrite = FALSE' if you do not want to overwrite it.
#> Overwriting 'spe$key'. Set 'overwrite = FALSE' if you do not want to overwrite it.
Examples
## Re-import them
colData(cluster_import(spe))
}
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+#> 2024-12-12 20:13:02.037251 loading file /github/home/.cache/R/BiocFileCache/104970f7ab1e_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
#> Overwriting 'spe$key'. Set 'overwrite = FALSE' if you do not want to overwrite it.
-#> Warning: '/tmp/RtmpHefYvC/exported_clusters/spatialLIBD' already exists
+#> Warning: '/tmp/Rtmpzq7cH3/exported_clusters/spatialLIBD' already exists
#> Overwriting 'spe$key'. Set 'overwrite = FALSE' if you do not want to overwrite it.
-#> Warning: '/tmp/RtmpHefYvC/exported_clusters/GraphBased' already exists
+#> Warning: '/tmp/Rtmpzq7cH3/exported_clusters/GraphBased' already exists
#> Overwriting 'spe$key'. Set 'overwrite = FALSE' if you do not want to overwrite it.
#> DataFrame with 47681 rows and 71 columns
#> key sample_id Cluster sum_umi
diff --git a/reference/enough_ram.html b/reference/enough_ram.html
index 27f42f20..fd08c505 100644
--- a/reference/enough_ram.html
+++ b/reference/enough_ram.html
@@ -17,7 +17,7 @@
diff --git a/reference/fetch_data.html b/reference/fetch_data.html
index 1baa67eb..13997af3 100644
--- a/reference/fetch_data.html
+++ b/reference/fetch_data.html
@@ -25,7 +25,7 @@
@@ -166,7 +166,7 @@ Examples
## Download the SingleCellExperiment object
## at the layer-level
if (!exists("sce_layer")) sce_layer <- fetch_data("sce_layer")
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+#> 2024-12-12 20:13:13.093641 loading file /github/home/.cache/R/BiocFileCache/5c77b6ee4a2_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
## Explore the data
sce_layer
diff --git a/reference/frame_limits.html b/reference/frame_limits.html
index b72f8c78..f14394bc 100644
--- a/reference/frame_limits.html
+++ b/reference/frame_limits.html
@@ -27,7 +27,7 @@
@@ -154,7 +154,7 @@ Examples
## Obtain the frame limits for one sample
frame_limits(spe, sampleid = "151673")
}
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+#> 2024-12-12 20:13:14.546558 loading file /github/home/.cache/R/BiocFileCache/104970f7ab1e_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
#> $y_min
#> [1] 64
#>
diff --git a/reference/gene_set_enrichment.html b/reference/gene_set_enrichment.html
index a3b02933..814233e0 100644
--- a/reference/gene_set_enrichment.html
+++ b/reference/gene_set_enrichment.html
@@ -22,7 +22,7 @@
@@ -177,7 +177,7 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
-#> 2024-12-12 20:08:00.259986 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:13:23.814359 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
## Compute the gene set enrichment results
asd_sfari_enrichment <- gene_set_enrichment(
diff --git a/reference/gene_set_enrichment_plot.html b/reference/gene_set_enrichment_plot.html
index c7cc26fd..9b723965 100644
--- a/reference/gene_set_enrichment_plot.html
+++ b/reference/gene_set_enrichment_plot.html
@@ -18,7 +18,7 @@
@@ -178,7 +178,7 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
-#> 2024-12-12 20:08:01.751422 loading file /github/home/.cache/R/BiocFileCache/5c643ff6574_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
+#> 2024-12-12 20:13:24.972142 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
## Compute the gene set enrichment results
asd_sfari_enrichment <- gene_set_enrichment(
diff --git a/reference/geom_spatial.html b/reference/geom_spatial.html
index 257198bd..9941483d 100644
--- a/reference/geom_spatial.html
+++ b/reference/geom_spatial.html
@@ -20,7 +20,7 @@
@@ -175,7 +175,7 @@ Examples
## Clean up
rm(spe_sub)
}
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+#> 2024-12-12 20:13:26.612894 loading file /github/home/.cache/R/BiocFileCache/104970f7ab1e_Human_DLPFC_Visium_processedData_sce_scran_spatialLIBD.Rdata%3Fdl%3D1
Examples
## Obtain the necessary data
if (!exists("sce_layer")) sce_layer <- fetch_data("sce_layer")
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## Example layer colors with the corresponding names
get_colors(libd_layer_colors, sce_layer$layer_guess)
diff --git a/reference/img_edit.html b/reference/img_edit.html
index b93dd331..ee4e7a98 100644
--- a/reference/img_edit.html
+++ b/reference/img_edit.html
@@ -19,7 +19,7 @@
Examples
x <- img_edit(spe, sampleid = "151507", brightness = 25)
plot(x)
}
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diff --git a/reference/img_update.html b/reference/img_update.html
index ccfd0ffa..6dd4b819 100644
--- a/reference/img_update.html
+++ b/reference/img_update.html
@@ -17,7 +17,7 @@
@@ -139,7 +139,7 @@ Examples
## Reduce brightness to 25% and update the imgData()
imgData(img_update(spe, sampleid = "151507", brightness = 25))
}
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#> DataFrame with 13 rows and 4 columns
#> sample_id image_id data scaleFactor
#> <character> <character> <list> <numeric>
diff --git a/reference/img_update_all.html b/reference/img_update_all.html
index db463905..61742976 100644
--- a/reference/img_update_all.html
+++ b/reference/img_update_all.html
@@ -19,7 +19,7 @@
@@ -138,7 +138,7 @@ Examples
## update the imgData()
imgData(img_update_all(spe, brightness = 25))
}
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#> DataFrame with 24 rows and 4 columns
#> sample_id image_id data scaleFactor
#> <character> <character> <list> <numeric>
diff --git a/reference/index.html b/reference/index.html
index 99adfd9a..d05abc94 100644
--- a/reference/index.html
+++ b/reference/index.html
@@ -17,7 +17,7 @@
diff --git a/reference/layer_boxplot.html b/reference/layer_boxplot.html
index 5ba33c11..7b878b7a 100644
--- a/reference/layer_boxplot.html
+++ b/reference/layer_boxplot.html
@@ -19,7 +19,7 @@
@@ -185,9 +185,9 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
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if (!exists("sce_layer")) sce_layer <- fetch_data(type = "sce_layer")
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+#> 2024-12-12 20:14:11.387783 loading file /github/home/.cache/R/BiocFileCache/5c77b6ee4a2_Human_DLPFC_Visium_processedData_sce_scran_sce_layer_spatialLIBD.Rdata%3Fdl%3D1
## Top 2 genes from the enrichment model
sig_genes <- sig_genes_extract_all(
diff --git a/reference/layer_matrix_plot.html b/reference/layer_matrix_plot.html
index 977af7a9..9ae63397 100644
--- a/reference/layer_matrix_plot.html
+++ b/reference/layer_matrix_plot.html
@@ -20,7 +20,7 @@
diff --git a/reference/layer_stat_cor.html b/reference/layer_stat_cor.html
index 2a1e7cdf..82e1456a 100644
--- a/reference/layer_stat_cor.html
+++ b/reference/layer_stat_cor.html
@@ -17,7 +17,7 @@
@@ -161,7 +161,7 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
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+#> 2024-12-12 20:14:14.297368 loading file /github/home/.cache/R/BiocFileCache/5c73806c84a_Human_DLPFC_Visium_modeling_results.Rdata%3Fdl%3D1
## Compute the correlations
cor_stats_layer <- layer_stat_cor(
diff --git a/reference/layer_stat_cor_plot.html b/reference/layer_stat_cor_plot.html
index 58ced45d..0556ad9b 100644
--- a/reference/layer_stat_cor_plot.html
+++ b/reference/layer_stat_cor_plot.html
@@ -22,7 +22,7 @@
@@ -169,13 +169,13 @@ Examples
if (!exists("modeling_results")) {
modeling_results <- fetch_data(type = "modeling_results")
}
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## query spatialDLPFC modeling results
query_modeling_results <- fetch_data(
type = "spatialDLPFC_Visium_modeling_results"
)
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+#> 2024-12-12 20:14:16.2476 loading file /github/home/.cache/R/BiocFileCache/104977134dbb_modeling_results_BayesSpace_k09.Rdata%3Fdl%3D1
## Compute the correlations
cor_stats_layer <- layer_stat_cor(
diff --git a/reference/libd_layer_colors.html b/reference/libd_layer_colors.html
index eaf1b279..760f52f4 100644
--- a/reference/libd_layer_colors.html
+++ b/reference/libd_layer_colors.html
@@ -18,7 +18,7 @@
diff --git a/reference/locate_images.html b/reference/locate_images.html
index 6f1939a8..b1ecc16a 100644
--- a/reference/locate_images.html
+++ b/reference/locate_images.html
@@ -19,7 +19,7 @@
diff --git a/reference/multi_gene_pca.html b/reference/multi_gene_pca.html
index 84ebcfd4..405a64bb 100644
--- a/reference/multi_gene_pca.html
+++ b/reference/multi_gene_pca.html
@@ -22,7 +22,7 @@
diff --git a/reference/multi_gene_sparsity.html b/reference/multi_gene_sparsity.html
index 53360092..db8dd059 100644
--- a/reference/multi_gene_sparsity.html
+++ b/reference/multi_gene_sparsity.html
@@ -18,7 +18,7 @@
diff --git a/reference/multi_gene_z_score.html b/reference/multi_gene_z_score.html
index 9888c85e..8be9ac0e 100644
--- a/reference/multi_gene_z_score.html
+++ b/reference/multi_gene_z_score.html
@@ -18,7 +18,7 @@
diff --git a/reference/prep_stitched_data.html b/reference/prep_stitched_data.html
index f5a55a75..5e27e99d 100644
--- a/reference/prep_stitched_data.html
+++ b/reference/prep_stitched_data.html
@@ -22,7 +22,7 @@
diff --git a/reference/read10xVisiumAnalysis.html b/reference/read10xVisiumAnalysis.html
index c521eb0c..b96b2175 100644
--- a/reference/read10xVisiumAnalysis.html
+++ b/reference/read10xVisiumAnalysis.html
@@ -18,7 +18,7 @@
diff --git a/reference/read10xVisiumWrapper.html b/reference/read10xVisiumWrapper.html
index 426d2d3b..eb68f1db 100644
--- a/reference/read10xVisiumWrapper.html
+++ b/reference/read10xVisiumWrapper.html
@@ -20,7 +20,7 @@
diff --git a/reference/registration_block_cor.html b/reference/registration_block_cor.html
index 177d3978..91b94e68 100644
--- a/reference/registration_block_cor.html
+++ b/reference/registration_block_cor.html
@@ -20,7 +20,7 @@
@@ -146,9 +146,9 @@ Examples
#>
#> rgstr_> ## Pseudo-bulk
#> rgstr_> sce_pseudo <- registration_pseudobulk(sce, "Cell_Cycle", "sample_id", c("age"), min_ncells = NULL)
-#> 2024-12-12 20:09:02.846125 make pseudobulk object
-#> 2024-12-12 20:09:02.971929 drop lowly expressed genes
-#> 2024-12-12 20:09:03.034428 normalize expression
+#> 2024-12-12 20:14:22.169827 make pseudobulk object
+#> 2024-12-12 20:14:22.290642 drop lowly expressed genes
+#> 2024-12-12 20:14:22.349012 normalize expression
#>
#> rgstr_> colData(sce_pseudo)
#> DataFrame with 20 rows and 8 columns
@@ -180,7 +180,7 @@ Examples
#> E_S S E 11
#>
#> rgstr_> registration_mod <- registration_model(sce_pseudo, "age")
-#> 2024-12-12 20:09:03.114902 create model matrix
+#> 2024-12-12 20:14:22.426173 create model matrix
#>
#> rgstr_> head(registration_mod)
#> registration_variableG0 registration_variableG1 registration_variableG2M
@@ -198,8 +198,8 @@ Examples
#> E_G0 0 20.97006
#> A_G1 0 19.18719
block_cor <- registration_block_cor(sce_pseudo, registration_mod)
-#> 2024-12-12 20:09:03.127392 run duplicateCorrelation()
-#> 2024-12-12 20:09:04.438658 The estimated correlation is: -0.0187869166526901
+#> 2024-12-12 20:14:22.438107 run duplicateCorrelation()
+#> 2024-12-12 20:14:23.737962 The estimated correlation is: -0.0187869166526901
diff --git a/reference/registration_model.html b/reference/registration_model.html
index 54d1ed66..d63be0e6 100644
--- a/reference/registration_model.html
+++ b/reference/registration_model.html
@@ -21,7 +21,7 @@
@@ -148,9 +148,9 @@ Examples
#>
#> rgstr_> ## Pseudo-bulk
#> rgstr_> sce_pseudo <- registration_pseudobulk(sce, "Cell_Cycle", "sample_id", c("age"), min_ncells = NULL)
-#> 2024-12-12 20:09:04.749012 make pseudobulk object
-#> 2024-12-12 20:09:04.879655 drop lowly expressed genes
-#> 2024-12-12 20:09:04.931246 normalize expression
+#> 2024-12-12 20:14:24.041494 make pseudobulk object
+#> 2024-12-12 20:14:24.170795 drop lowly expressed genes
+#> 2024-12-12 20:14:24.215742 normalize expression
#>
#> rgstr_> colData(sce_pseudo)
#> DataFrame with 20 rows and 8 columns
@@ -181,7 +181,7 @@ Examples
#> D_S S D 14
#> E_S S E 11
registration_mod <- registration_model(sce_pseudo, "age")
-#> 2024-12-12 20:09:05.012922 create model matrix
+#> 2024-12-12 20:14:24.289774 create model matrix
head(registration_mod)
#> registration_variableG0 registration_variableG1 registration_variableG2M
#> A_G0 1 0 0
diff --git a/reference/registration_pseudobulk.html b/reference/registration_pseudobulk.html
index c113b746..5cdce7c4 100644
--- a/reference/registration_pseudobulk.html
+++ b/reference/registration_pseudobulk.html
@@ -18,7 +18,7 @@
@@ -97,7 +97,9 @@ Arguments
character(1)
specifying the colData(sce)
variable of interest against which will be used for computing the relevant
-statistics.
A character(1)
specifying the colData(sce)
variable of interest against which will be used for computing the relevant
-statistics.