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to generate a MIP image from an 8-bit JFRC2010 registered stack. But not long after I start the macro, I get an error:
"Plugin or class not found: "8bit_Gray_Clip_plugins.Mask_Brighness_Measure"
(java.lang.ClassNotFoundException: 8bit_Gray_Clip_plugins.Mask_Brighness_Measure)"
Could you send me your sample data? My email is [email protected]
The code has been adjusted for the 12bit data. Could be some issue with the 8bit less GAL4 expressed data.
I tried to use https://github.com/JaneliaSciComp/ColorMIP_Mask_Search/tree/master/ColorDepthMIP_Generator
to generate a MIP image from an 8-bit JFRC2010 registered stack. But not long after I start the macro, I get an error:
"Plugin or class not found: "8bit_Gray_Clip_plugins.Mask_Brighness_Measure"
(java.lang.ClassNotFoundException: 8bit_Gray_Clip_plugins.Mask_Brighness_Measure)"
I'm using Fiji version: 2.0.0-rc-67/1.52d.
Automatic Brightness adjustment ticked, DSLT version:line
Could you suggest a fix please?
Log:
Choose a directory for aligned confocal files
Choose a directory for Color MIP SAVE
Confocal Stack; /Users/mmc46/projects/SPN/images/
MIP saving place; /Users/mmc46/projects/SPN/Color_MIP/
filepath; /tmp/MIP_batch.txt
Desired mean; 190
JFRCpath; /Applications/Fiji.app/plugins/Plugins_Color_MIP/JFRC2010_Mask.tif
VncMaskpathF; /Applications/Fiji.app/plugins/Plugins_Color_MIP/Mask_VNC_Female.tif
VncMaskpathM; /Applications/Fiji.app/plugins/Plugins_Color_MIP/Mask_VNC_Male.tif
JFRC3Dpath; /Applications/Fiji.app/plugins/Plugins_Color_MIP/JFRC2010_3D_Mask.nrrd
JFRC2_SPN-split-vectashield-brain-2_02_warp_m0g80c8e1e-1x26r4.nrrd ; 0 / 1 TRY
JFRC2_SPN-split-vectashield-brain-2_02_warp_m0g80c8e1e-1x26r4.nrrd ; 0 / 1 opened
UseSubfolderName; 0
All channel MIP mode
Channel number; 1
8bit file
Maxvalue; 255 Minvalue; 0
3D stack brightness adjusted; maxvalue; 255 Inimax; 255
basicoperation done
Anisotropic diffusion ON
Area 1st time; 49207 mean; 255 totalpix; 472878 presize; 10.4059 % bitd; 8
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