diff --git a/index.md.bk b/index.md.bk
index f2e8b16..e1b25e4 100644
--- a/index.md.bk
+++ b/index.md.bk
@@ -64,6 +64,7 @@
     * [Intro to Maker Gene Prediction](dataAnalysis/GenomeAnnotation/Intro_To_Maker.md)
     * [Intro to Braker2 Gene Prediction](dataAnalysis/GenomeAnnotation/Intro_to_Braker2.md)
     * [Motif Identification and Finding with MEME and FIMO](dataAnalysis/GenomeAnnotation/MEME_Motif_Finding_In_Genomes.md)
+    * [How to identify the secreted protein from an annotation and predict subcellular localization](dataAnalysis/GenomeAnnotation/Secreted_Protein_Prediction_with_SignalP_and_TMHMM.md)
   * RNA-Seq Analysis
     * [RNA-Seq Example with a Genome Assembly](dataAnalysis/RNA-Seq/RNA-SeqIntro/RNAseq-using-a-genome.md): Arabidopsis with a Genome
     * [RNA-Seq Example without a Genome Assembly](dataAnalysis/RNA-Seq/RNA-SeqIntro/RNAseq-without-a-genome.md): Arabidopsis without a Genome