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m6Anet giving output with NaNs in the table #175

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baibhav-bioinfo opened this issue Oct 11, 2024 · 1 comment
Open

m6Anet giving output with NaNs in the table #175

baibhav-bioinfo opened this issue Oct 11, 2024 · 1 comment

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@baibhav-bioinfo
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baibhav-bioinfo commented Oct 11, 2024

Hi,

I am working on finding out the differential m6A modification in between control and drought samples with 3 repllicates.
I have used genome.fa in alignment and also in eventalign using nanopolish.

then i used eventlaign.txt as input in m6Anet.
the output i got from m6anet "inference" is containing "NaN" in the probability column.

why is that??
did i did a mistake by not using transcript.fasta rather than genome.fasta.

@yuukiiwa
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Hi @baibhav-bioinfo,

m6anet supports cDNA transcript.fa not genome.fa, so by changing to transcript.fa, it should fix it.

Thanks!

Best wishes,
Yuk Kei

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