Usage recommandations to filter out reads

[aghozlane]

Hello,

I have 15 technical control samples that I would like to use to filter reads occurring in real samples.
Basically I want to remove every reads with a high similarity with this control set of reads.

I initially concatenated all my files into two pairs and attempted to create the rconnector base. However, even with 200G of memory, this step does not pass. Do you have any recommendations ?