diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml index 6c3117206..f9430e6e8 100644 --- a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml @@ -1,13 +1,21 @@ - doc: Test outline for Assembly-Hifi-HiC-phasing-VGP4 job: - HiC forward reads: - class: File - location: https://zenodo.org/records/10068595/files/HiC%20forward%20reads.fastqsanger.gz?download=1 - filetype: fastqsanger.gz - HiC reverse reads: - class: File - location: https://zenodo.org/records/10068595/files/HiC%20reverse%20reads.fastqsanger.gz?download=1 - filetype: fastqsanger.gz + Hi-C Forward reads: + class: Collection + collection_type: list + elements: + - class: File + identifier: HiC-Forward-reads + location: https://zenodo.org/records/10068595/files/HiC%20forward%20reads.fastqsanger.gz?download=1 + filetype: fastqsanger.gz + Hi-C Reverse reads: + class: Collection + collection_type: list + elements: + - class: File + identifier: HiC-Reverse-reads + location: https://zenodo.org/records/10068595/files/HiC%20reverse%20reads.fastqsanger.gz?download=1 + filetype: fastqsanger.gz Genomescope Model Parameters: class: File path: test-data/Genomescope Model Parameters.tabular @@ -20,7 +28,7 @@ class: File location: https://zenodo.org/records/10068595/files/Meryl%20Database.meryldb?download=1 filetype: meryldb - Pacbio Reads Collection: + Pacbio Reads: class: Collection collection_type: list elements: @@ -33,26 +41,27 @@ Name for Haplotype 2: Hap2 Homozygous Read Coverage: null Database for Busco Lineage: v5 + Trim Hi-C reads?: false outputs: - Hifiasm HiC hap1: - assert: - has_n_line: - n: 168 + Hifiasm Hi-C hap1: + asserts: + has_n_lines: + n: 114 Estimated Genome size: 2288021 Busco Summary Hap1: asserts: has_text: - text: "C:1.0%[S:1.0%,D:0.0%],F:0.4%,M:98.6%,n:3354" + text: "C:0.8%[S:0.8%,D:0.0%],F:0.3%,M:98.9%,n:3354" Nx Plot: asserts: has_size: value: 65000 delta: 10000 usable hap1 gfa: - assert: - has_n_line: - n: 173 + asserts: + has_n_lines: + n: 119 Assembly statistics for Hap1 and Hap2: asserts: has_text: - text: "# scaffolds 72 35" + text: "# scaffolds 57 51" diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/CHANGELOG.md b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/CHANGELOG.md index 048600877..6f8dee847 100644 --- a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/CHANGELOG.md +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/CHANGELOG.md @@ -1,5 +1,21 @@ # Changelog +## [0.2.3] 2024-12-13 + +### Changes + +- Hi-C inputs are now entered as collections instead of single datasets +- Add optional trimming of Hi-C reads + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/2.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/column_maker/Add_a_column1/2.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.24.1+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.9+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.8.0+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cut_tool/9.3+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cut_tool/9.3+galaxy2` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.9+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.20.0+galaxy0` + + ## [0.2.2] 2024-08-07 - Expose parameter: Busco lineage database diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md index 1cc40e5db..1437a178a 100644 --- a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md @@ -5,8 +5,8 @@ Generate phased assembly based on PacBio Hifi Reads using HiC data from the same ## Inputs 1. Hifi long reads [fastq] -2. HiC forward reads (if multiple input files, concatenated in same order as reverse reads) [fastq] -3. HiC reverse reads (if multiple input files, concatenated in same order as forward reads) [fastq] +2. Collection of Hi-C forward reads [fastq] +3. Collection of Hi-C reverse reads [fastq] 4. K-mer database [meryldb] 5. Genome profile summary generated by Genomescope [txt] 6. Name of first assembly @@ -16,8 +16,10 @@ Generate phased assembly based on PacBio Hifi Reads using HiC data from the same 1. Haplotype 1 assembly ([fasta] and [gfa]) 2. Haplotype 2 assembly ([fasta] and [gfa]) -3. QC: BUSCO report for both assemblies -4. QC: Merqury report for both assemblies -5. QC: Assembly statistics for both assemblies -6. QC: Nx plot for both assemblies -7. QC: Size plot for both assemblies \ No newline at end of file +3. Trimmed Hi-C forward reads collection +4. Trimmed Hi-C reverse reads collection +5. QC: BUSCO report for both assemblies +6. QC: Merqury report for both assemblies +7. QC: Assembly statistics for both assemblies +8. QC: Nx plot for both assemblies +9. QC: Size plot for both assemblies