diff --git a/workflows/VGP-assembly-v2/Purgedups-VGP6/.dockstore.yml b/workflows/VGP-assembly-v2/Purgedups-VGP6/.dockstore.yml
new file mode 100644
index 000000000..a00df3b84
--- /dev/null
+++ b/workflows/VGP-assembly-v2/Purgedups-VGP6/.dockstore.yml
@@ -0,0 +1,7 @@
+version: 1.2
+workflows:
+- name: main
+  primaryDescriptorPath: /Purgedups-WF6.ga
+  subclass: Galaxy
+  testParameterFiles:
+  - /Purgedups-WF6-tests.yml
diff --git a/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6-tests.yml b/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6-tests.yml
new file mode 100644
index 000000000..b6ed07da4
--- /dev/null
+++ b/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6-tests.yml
@@ -0,0 +1,77 @@
+- doc: Test outline for Purgedups-WF6
+  job:
+    Hifiasm Primary assembly:
+      class: File
+      location: https://www.dropbox.com/scl/fi/9vszjjc2s2wjyynwzdday/Hifiasm-Primary-assembly.fasta?rlkey=iikx1zf3makx8tea2rvfdz5bv&dl=1
+      filetype: fasta
+    Hifiasm Alternate assembly:
+      class: File
+      location: https://www.dropbox.com/scl/fi/rlikmkfwjdl2fk584xcoy/Hifiasm-Alternate-assembly.fasta?rlkey=8629bdqyt10te3dvlnho0vgdw&dl=1
+      filetype: fasta
+    Meryl Database:
+      class: File
+      location: https://www.dropbox.com/scl/fi/9nkb5rgo15bq7qug81sve/Meryl-Database.meryldb?rlkey=bhcjv65tqg5ebaavvybscztl1&dl=1
+      filetype: meryldb
+    Genomescope model parameters:
+      class: File
+      location: https://www.dropbox.com/scl/fi/q8p0donxzvcv7b1wrn7xp/Genomescope-model-parameters.tabular?rlkey=2wjo0ymxiay51danj715dtsbp&dl=1
+      filetype: tabular
+    Estimated genome size - Parameter File:
+      class: File
+      location: https://www.dropbox.com/scl/fi/es8yn0vl2gp3crilfbj1y/Estimated-genome-size-Parameter-File.expression.json?rlkey=p83ky9xa72fqrr4dm5cpgza5f&dl=1
+      filetype: expression.json
+    Pacbio Reads Collection - Trimmed:
+      class: Collection
+      collection_type: list
+      elements:
+      - class: File
+        identifier: yeast_reads_sub1.fastq.gz
+        location: https://www.dropbox.com/scl/fi/lurqcq79www0e1g4e3qo3/Pacbio-Reads-Collection-Trimmed_yeast_reads_sub1.fastq.gz.fastq.gz?rlkey=nfbvfr0jcpknj66mp2fb0xsv2&dl=1
+    Busco Lineage: vertebrata_odb10
+    SAK input file (Optional): null
+    Name of primary assembly: Primary
+    Name of alternate assembly: Alternate
+  outputs:
+    Cutoffs for primary assembly:
+      asserts:
+        has_text:
+          text: "1	15	15	16	16	48"
+    Purged Primary Assembly:
+      asserts:
+        has_n_lines:
+          n: 156
+    Purged Primary Assembly (gfa):
+      asserts:
+        has_n_lines:
+          n: 235
+    'Busco on Purged Primary assembly: short summary':
+      asserts:
+        has_text:
+          text: "C:1.1%[S:1.1%,D:0.0%],F:0.5%,M:98.4%,n:3354"
+    Cutoffs for alternate assembly:
+      asserts:
+        has_text:
+          text: "1	15	15	16	16	48"
+    Purged Alternate assembly:
+      asserts:
+        has_n_lines:
+          n: 144
+    Purged Alternate assembly (gfa):
+      asserts:
+        has_n_lines:
+          n: 217
+    Assembly stats on Primary and alternate assemblies:
+      asserts:
+        has_text:
+          text: "# contigs	78	72"
+    Nx Plot:
+      asserts:
+        has_size: 
+          value : 61000
+          delta: 5000
+    'Merqury on Phased assemblies: stats':
+      element_tests:
+        output_merqury.completeness:
+         asserts:
+          has_text:
+            text: "# contigs	78	72"
diff --git a/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6.ga b/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6.ga
new file mode 100644
index 000000000..419e1a354
--- /dev/null
+++ b/workflows/VGP-assembly-v2/Purgedups-VGP6/Purgedups-WF6.ga
@@ -0,0 +1,3462 @@
+{
+    "a_galaxy_workflow": "true",
+    "annotation": "",
+    "creator": [
+        {
+            "class": "Organization",
+            "name": "Galaxy"
+        },
+        {
+            "class": "Organization",
+            "name": "VGP",
+            "url": "https://vertebrategenomeproject.org"
+        }
+    ],
+    "format-version": "0.1",
+    "license": "CC-BY-4.0",
+    "release": "0.1",
+    "name": "Purgedups-WF6",
+    "steps": {
+        "0": {
+            "annotation": "",
+            "content_id": null,
+            "errors": null,
+            "id": 0,
+            "input_connections": {},
+            "inputs": [
+                {
+                    "description": "",
+                    "name": "Pacbio Reads Collection - Trimmed"
+                }
+            ],
+            "label": "Pacbio Reads Collection - Trimmed",
+            "name": "Input dataset collection",
+            "outputs": [],
+            "position": {
+                "left": 6.078125,
+                "top": 0
+            },
+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"tag\": \"\", \"collection_type\": \"list\"}",
+            "tool_version": null,
+            "type": "data_collection_input",
+            "uuid": "65452546-c777-41af-82c3-a90d46d782d8",
+            "when": null,
+            "workflow_outputs": []
+        },
+        "1": {
+            "annotation": "",
+            "content_id": null,
+            "errors": null,
+            "id": 1,
+            "input_connections": {},
+            "inputs": [
+                {
+                    "description": "",
+                    "name": "Hifiasm Primary assembly"
+                }
+            ],
+            "label": "Hifiasm Primary assembly",
+            "name": "Input dataset",
+            "outputs": [],
+            "position": {
+                "left": 0,
+                "top": 111.5
+            },
+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"format\": [\"fasta\"], \"tag\": \"\"}",
+            "tool_version": null,
+            "type": "data_input",
+            "uuid": "ddf397f4-eb8e-4945-8034-89ef98a195c5",
+            "when": null,
+            "workflow_outputs": []
+        },
+        "2": {
+            "annotation": "",
+            "content_id": null,
+            "errors": null,
+            "id": 2,
+            "input_connections": {},
+            "inputs": [
+                {
+                    "description": "",
+                    "name": "Hifiasm Alternate assembly"
+                }
+            ],
+            "label": "Hifiasm Alternate assembly",
+            "name": "Input dataset",
+            "outputs": [],
+            "position": {
+                "left": 14.171875,
+                "top": 210.734375
+            },
+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"format\": [\"fasta\"], \"tag\": \"\"}",
+            "tool_version": null,
+            "type": "data_input",
+            "uuid": "618f3ea7-e809-4c8a-b30e-04a4581a839a",
+            "when": null,
+            "workflow_outputs": []
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+        "3": {
+            "annotation": "",
+            "content_id": null,
+            "errors": null,
+            "id": 3,
+            "input_connections": {},
+            "inputs": [
+                {
+                    "description": "",
+                    "name": "Meryl Database"
+                }
+            ],
+            "label": "Meryl Database",
+            "name": "Input dataset",
+            "outputs": [],
+            "position": {
+                "left": 14.171875,
+                "top": 315.234375
+            },
+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"tag\": \"\"}",
+            "tool_version": null,
+            "type": "data_input",
+            "uuid": "ee9e9447-4e5f-4368-a449-70167c3b36c6",
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+            "content_id": null,
+            "errors": null,
+            "id": 4,
+            "input_connections": {},
+            "inputs": [
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+                    "description": "",
+                    "name": "Genomescope model parameters"
+                }
+            ],
+            "label": "Genomescope model parameters",
+            "name": "Input dataset",
+            "outputs": [],
+            "position": {
+                "left": 16.078125,
+                "top": 428.125
+            },
+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"tag\": \"\"}",
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+            "type": "data_input",
+            "uuid": "d37b371a-8582-4b11-b458-659851a1f0a3",
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+                    "name": "Estimated genome size - Parameter File"
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+            ],
+            "label": "Estimated genome size - Parameter File",
+            "name": "Input dataset",
+            "outputs": [],
+            "position": {
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+                "top": 579.9375
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+            "tool_id": null,
+            "tool_state": "{\"optional\": false, \"tag\": \"\"}",
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+            "id": 6,
+            "input_connections": {},
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+                {
+                    "description": "",
+                    "name": "Busco Lineage"
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+            ],
+            "label": "Busco Lineage",
+            "name": "Input parameter",
+            "outputs": [],
+            "position": {
+                "left": 1202.3213024806632,
+                "top": 1405.449719429178
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+            "tool_id": null,
+            "tool_state": "{\"restrictOnConnections\": true, \"parameter_type\": \"text\", \"optional\": false}",
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+            "id": 7,
+            "input_connections": {},
+            "inputs": [
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+                    "description": "",
+                    "name": "SAK input file (Optional)"
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+            "label": "SAK input file (Optional)",
+            "name": "Input dataset",
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+            "id": 8,
+            "input_connections": {},
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+                    "description": "",
+                    "name": "Name of primary assembly"
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+            ],
+            "label": "Name of primary assembly",
+            "name": "Input parameter",
+            "outputs": [],
+            "position": {
+                "left": 4422.09375,
+                "top": 201.9375
+            },
+            "tool_id": null,
+            "tool_state": "{\"default\": \"Primary\", \"parameter_type\": \"text\", \"optional\": true}",
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+                "top": 321.09375
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+            "tool_state": "{\"default\": \"Alternate\", \"parameter_type\": \"text\", \"optional\": true}",
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+                        "uuid": "21466675-1372-43d4-a5cc-9fb8866b7cc1",
+                        "when": null,
+                        "workflow_outputs": [
+                            {
+                                "label": "Size Plot",
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+        }
+    },
+    "tags": [
+        "VGP_curated"
+    ],
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+}
\ No newline at end of file