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no-hit 80% #135

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Ofsm opened this issue Oct 18, 2024 · 0 comments
Open

no-hit 80% #135

Ofsm opened this issue Oct 18, 2024 · 0 comments

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@Ofsm
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Ofsm commented Oct 18, 2024

Hi, I'm running a test with some WGS short-reads insects data, everything looks good, but blobtools results showed around 80% no-hit. Why?

Here is the command I used:
##BLAST
blastn -query KST141.fasta -db core_nt -outfmt "6 qseqid staxids bitscore std sscinames sskingdoms stitle" -max_hsps 1 -evalue 1e-25 -out KST141_blast.out -num_threads 30

##BLOBTOOLS
blobtools create --infile KST141.fasta --hitsfile KST141_blast.out --bam minimap2_KST141_sorted.bam --nodes /home/os/Metawrap/KST141/taxdump/nodes.dmp --names /home/os/Metawrap/KST141/taxdump/names.dmp --out KST141 --title BlobDB
blobtools view --input KST141.blobDB.json
blobtools plot --infile KST141.blobDB.json

Thanks for the help!

BlobDB bestsum phylum p8 span 100 blobplot bam0
BlobDB bestsum phylum p8 span 100 blobplot read_cov bam0

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