We read every piece of feedback, and take your input very seriously.
To see all available qualifiers, see our documentation.
Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.
By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.
Already on GitHub? Sign in to your account
Hi, I'm running a test with some WGS short-reads insects data, everything looks good, but blobtools results showed around 80% no-hit. Why?
Here is the command I used: ##BLAST blastn -query KST141.fasta -db core_nt -outfmt "6 qseqid staxids bitscore std sscinames sskingdoms stitle" -max_hsps 1 -evalue 1e-25 -out KST141_blast.out -num_threads 30
##BLOBTOOLS blobtools create --infile KST141.fasta --hitsfile KST141_blast.out --bam minimap2_KST141_sorted.bam --nodes /home/os/Metawrap/KST141/taxdump/nodes.dmp --names /home/os/Metawrap/KST141/taxdump/names.dmp --out KST141 --title BlobDB blobtools view --input KST141.blobDB.json blobtools plot --infile KST141.blobDB.json
Thanks for the help!
The text was updated successfully, but these errors were encountered:
No branches or pull requests
Hi, I'm running a test with some WGS short-reads insects data, everything looks good, but blobtools results showed around 80% no-hit. Why?
Here is the command I used:
##BLAST
blastn -query KST141.fasta -db core_nt -outfmt "6 qseqid staxids bitscore std sscinames sskingdoms stitle" -max_hsps 1 -evalue 1e-25 -out KST141_blast.out -num_threads 30
##BLOBTOOLS
blobtools create --infile KST141.fasta --hitsfile KST141_blast.out --bam minimap2_KST141_sorted.bam --nodes /home/os/Metawrap/KST141/taxdump/nodes.dmp --names /home/os/Metawrap/KST141/taxdump/names.dmp --out KST141 --title BlobDB
blobtools view --input KST141.blobDB.json
blobtools plot --infile KST141.blobDB.json
Thanks for the help!
The text was updated successfully, but these errors were encountered: