--help
: List all available parameters and the default values
--read_path
: Path to a folder containing all input fastq files (this will be recursively searched for *fastq.gz/*fq.gz/*fq/*fastq files)--reads
: Glob pattern to match reads, e.g. '/path/to/reads_*{R1,R2}.fq.gz' (this must be quoted and is in conflict with--read_path
)
--outdir
: Pipeline output directory
--decont_off
: Skip trimming and decontamination--decont_ref_path
: Path to the host reference database--decont_index
: BWA index prefix for the host
--profilers
: Metagenomics profilers to run. This can be empty, i.e. only run decontamination or a list of profilers delimited with,
, e.g.metaphlan2,humann2,kraken2
. By default, the pipeline runs all available profilers.
--kraken2_index
: Path to the kraken2 database
--metaphlan2_ref_path
: Path to the metaphlan2 database--metaphlan2_index
: Bowtie2 index prefix for the marker genes--metaphlan2_pkl
: Python pickle file for marker genes
--humann2_nucleotide
: Path to humann2 chocophlan database--humann2_protein
: Path to humann2 protein database
--srst2_ref
: Fasta file used for srst2
--awsqueue
: The AWSBatch JobQueue that needs to be set when running on AWSBatch--awsregion
: The AWS Region for your AWS Batch job to run on