You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
I am working with Lofreq2.1.2. I run the following command to call only indels:
bin/lofreq call-parallel --pp-threads $threads $input -f ${reference} -o ${filename}_indel.vcf -Q 20 -B -m 17 -a 0.01 --only-indels --no-default-filter --call-indels
From intermediate files I observed that indel are called, however, even if I specified "--no-default-filter" option, lofreq applies lofreq filter. The result is the loss of all the indels called since all have QUAL lower the default --snvqual-thresh. It is fundamental to me leaving all the raw indel calls since I am working on realign reads to call indel.
I thank you in advance for your willingness.
The text was updated successfully, but these errors were encountered:
Hi, I am having the same issues also when using lofreq call. I observed that basically lofreq does not use the arguments I provide. It does not use --no-default-filter and it does not use --force-overwrite, so processes always throw the error "Cowardly refusing to overwrite exsiting file".
I am working with Lofreq2.1.2. I run the following command to call only indels:$input -f $ {reference} -o ${filename}_indel.vcf -Q 20 -B -m 17 -a 0.01 --only-indels --no-default-filter --call-indels
bin/lofreq call-parallel --pp-threads $threads
From intermediate files I observed that indel are called, however, even if I specified "--no-default-filter" option, lofreq applies lofreq filter. The result is the loss of all the indels called since all have QUAL lower the default --snvqual-thresh. It is fundamental to me leaving all the raw indel calls since I am working on realign reads to call indel.
I thank you in advance for your willingness.
The text was updated successfully, but these errors were encountered: