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How does vaf cutoff influence the result? #185

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EvaPang2022 opened this issue May 28, 2023 · 0 comments
Open

How does vaf cutoff influence the result? #185

EvaPang2022 opened this issue May 28, 2023 · 0 comments

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@EvaPang2022
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Hi,

I ran vardict for three times setting individual allele frequency -f as 0, 0.01 and 0.1.

In the output vcf file, the number of records for these three runs are as follow:
-f 0: 492726
-f 0.01: 455223
-f 0.1: 187461

At the begining, I thought that only those mutations with vcf>cutoff were shown in the vcf file. But I found that in the three vcf files, there were still mutations with vaf less than the cutoff and they were marked as 'f0.1/f0.01/f0' in the FILTER column.

May I know what accounts for the different number of records?

Thanks,
Eva

@EvaPang2022 EvaPang2022 changed the title How does vcf cutoff influence the result? How does vaf cutoff influence the result? Jun 12, 2023
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