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I ran vardict for three times setting individual allele frequency -f as 0, 0.01 and 0.1.
In the output vcf file, the number of records for these three runs are as follow:
-f 0: 492726
-f 0.01: 455223
-f 0.1: 187461
At the begining, I thought that only those mutations with vcf>cutoff were shown in the vcf file. But I found that in the three vcf files, there were still mutations with vaf less than the cutoff and they were marked as 'f0.1/f0.01/f0' in the FILTER column.
May I know what accounts for the different number of records?
Thanks,
Eva
The text was updated successfully, but these errors were encountered:
EvaPang2022
changed the title
How does vcf cutoff influence the result?
How does vaf cutoff influence the result?
Jun 12, 2023
Hi,
I ran vardict for three times setting individual allele frequency -f as 0, 0.01 and 0.1.
In the output vcf file, the number of records for these three runs are as follow:
-f 0: 492726
-f 0.01: 455223
-f 0.1: 187461
At the begining, I thought that only those mutations with vcf>cutoff were shown in the vcf file. But I found that in the three vcf files, there were still mutations with vaf less than the cutoff and they were marked as 'f0.1/f0.01/f0' in the FILTER column.
May I know what accounts for the different number of records?
Thanks,
Eva
The text was updated successfully, but these errors were encountered: