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Describe the bug
When I run ‘perl METABOLIC-C.pl -in-gn /data_SSD/home/newshy/Metabolic/Sharp/test -r /data_SSD/home/newshy/Metabolic/test2.txt -o result3 -t 15’, a bug occurs. My input file is a fasta file. However, the error in the log is ‘Error: reads file does not look like a FASTQ file’.
[2024-10-13 22:57:20] Drawing element cycling diagrams...
Renaming result4/All_gene_collections.gene.scaffold.3.bt2.tmp to result4/All_gene_collections.gene.scaffold.3.bt2
Renaming result4/All_gene_collections.gene.scaffold.4.bt2.tmp to result4/All_gene_collections.gene.scaffold.4.bt2
Renaming result4/All_gene_collections.gene.scaffold.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.3.bt2.tmp to result4/All_gene_collections.gene.scaffold.3.bt2
Renaming result4/All_gene_collections.gene.scaffold.4.bt2.tmp to result4/All_gene_collections.gene.scaffold.4.bt2
Renaming result4/All_gene_collections.gene.scaffold.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.2.bt2
Error: reads file does not look like a FASTQ file
Error: reads file does not look like a FASTQ file
terminate called after throwing an instance of 'int'
terminate called after throwing an instance of 'int'
(ERR): bowtie2-align died with signal 6 (ABRT) (core dumped)
(ERR): bowtie2-align died with signal 6 (ABRT) (core dumped)
Illegal division by zero at METABOLIC-C.pl line 2097.
Illegal division by zero at METABOLIC-C.pl line 2097.
Expected behavior
A clear and concise description of what you expected to happen.
Screenshots
If applicable, add screenshots to help explain your problem.
Additional context
METABOLIC.log
The text was updated successfully, but these errors were encountered:
Hi, ----------"My input file is a fasta file. However, the error in the log is ‘Error: reads file does not look like a FASTQ file’."
Input file should be a FASTQ file, not a fasta file, two different type files.
Describe the bug
When I run ‘perl METABOLIC-C.pl -in-gn /data_SSD/home/newshy/Metabolic/Sharp/test -r /data_SSD/home/newshy/Metabolic/test2.txt -o result3 -t 15’, a bug occurs. My input file is a fasta file. However, the error in the log is ‘Error: reads file does not look like a FASTQ file’.
To Reproduce
Steps to reproduce the behavior:
Renaming result4/All_gene_collections.gene.scaffold.3.bt2.tmp to result4/All_gene_collections.gene.scaffold.3.bt2
Renaming result4/All_gene_collections.gene.scaffold.4.bt2.tmp to result4/All_gene_collections.gene.scaffold.4.bt2
Renaming result4/All_gene_collections.gene.scaffold.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.3.bt2.tmp to result4/All_gene_collections.gene.scaffold.3.bt2
Renaming result4/All_gene_collections.gene.scaffold.4.bt2.tmp to result4/All_gene_collections.gene.scaffold.4.bt2
Renaming result4/All_gene_collections.gene.scaffold.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.2.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.1.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.1.bt2
Renaming result4/All_gene_collections.gene.scaffold.rev.2.bt2.tmp to result4/All_gene_collections.gene.scaffold.rev.2.bt2
Error: reads file does not look like a FASTQ file
Error: reads file does not look like a FASTQ file
terminate called after throwing an instance of 'int'
terminate called after throwing an instance of 'int'
(ERR): bowtie2-align died with signal 6 (ABRT) (core dumped)
(ERR): bowtie2-align died with signal 6 (ABRT) (core dumped)
Illegal division by zero at METABOLIC-C.pl line 2097.
Illegal division by zero at METABOLIC-C.pl line 2097.
Expected behavior
A clear and concise description of what you expected to happen.
Screenshots
If applicable, add screenshots to help explain your problem.
Additional context
METABOLIC.log
The text was updated successfully, but these errors were encountered: