From 03a784c56f5289284f923693afb93a2ad033e17a Mon Sep 17 00:00:00 2001 From: Christopher Morrison Date: Wed, 25 Oct 2023 14:51:40 -0700 Subject: [PATCH 1/4] Remove skip and test lims consistency with new data. Improve metadata symmetry of typing between S3 load and LIMS. Add column order to lims. --- .../behavior_project_cache.py | 2 +- .../data_io/behavior_project_cloud_api.py | 45 ++++++++--- .../data_io/behavior_project_lims_api.py | 80 +++++++++++++++---- .../ophys/project_constants.py | 49 ++++++------ allensdk/core/dataframe_utils.py | 7 +- .../test_behavior_project_lims_api.py | 7 +- 6 files changed, 129 insertions(+), 61 deletions(-) diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py b/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py index cea0a7125..6b6aa2494 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py @@ -74,7 +74,7 @@ class VBOLimsCache(Cache): class VisualBehaviorOphysProjectCache(ProjectCacheBase): PROJECT_NAME = "visual-behavior-ophys" - BUCKET_NAME = "staging.visual-behavior-ophys-data" + BUCKET_NAME = "visual-behavior-ophys-data" def __init__( self, diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py index 3c6c74783..17652fa74 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py @@ -24,22 +24,17 @@ BehaviorSession, ) from allensdk.core.dataframe_utils import ( + enforce_df_column_order, enforce_df_int_typing, return_one_dataframe_row_only, ) from allensdk.core.utilities import literal_col_eval +from allensdk.brain_observatory.ophys.project_constants import ( + VBO_METADATA_COLUMN_ORDER, + VBO_INTEGER_COLUMNS +) COL_EVAL_LIST = ["ophys_experiment_id", "ophys_container_id", "driver_line"] -INTEGER_COLUMNS = [ - "session_number", - "prior_exposures_to_image_set", - "ophys_session_id", - "imaging_plane_group_count", - "imaging_plane_group", - "targeted_areas", - "num_depths_per_area", - "num_targeted_structures", -] def sanitize_data_columns( @@ -194,7 +189,15 @@ def _get_ophys_session_table(self): df["date_of_acquisition"] = pd.to_datetime( df["date_of_acquisition"], utc="True" ) - df = enforce_df_int_typing(df, INTEGER_COLUMNS, True) + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + df = enforce_df_column_order( + input_df=df, + column_order=VBO_METADATA_COLUMN_ORDER + ) self._ophys_session_table = df.set_index("ophys_session_id") def get_ophys_session_table(self) -> pd.DataFrame: @@ -219,7 +222,15 @@ def _get_behavior_session_table(self): df["date_of_acquisition"] = pd.to_datetime( df["date_of_acquisition"], utc="True" ) - df = enforce_df_int_typing(df, INTEGER_COLUMNS, True) + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + df = enforce_df_column_order( + input_df=df, + column_order=VBO_METADATA_COLUMN_ORDER + ) self._behavior_session_table = df.set_index("behavior_session_id") @@ -249,7 +260,15 @@ def _get_ophys_experiment_table(self): df["date_of_acquisition"] = pd.to_datetime( df["date_of_acquisition"], utc="True" ) - df = enforce_df_int_typing(df, INTEGER_COLUMNS, True) + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + df = enforce_df_column_order( + input_df=df, + column_order=VBO_METADATA_COLUMN_ORDER + ) self._ophys_experiment_table = df.set_index("ophys_experiment_id") def _get_ophys_cells_table(self): diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py index d7451df04..b0a15a06a 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py @@ -24,6 +24,14 @@ build_in_list_selector_query, build_where_clause, ) +from allensdk.core.dataframe_utils import ( + enforce_df_column_order, + enforce_df_int_typing +) +from allensdk.brain_observatory.ophys.project_constants import ( + VBO_METADATA_COLUMN_ORDER, + VBO_INTEGER_COLUMNS +) class BehaviorProjectLimsApi(BehaviorProjectBase): @@ -384,7 +392,17 @@ def _get_ophys_experiment_table(self) -> pd.DataFrame: .astype(int) ) targeted_imaging_depth.columns = ["targeted_imaging_depth"] - return query_df.merge(targeted_imaging_depth, on="ophys_container_id") + df = query_df.merge(targeted_imaging_depth, on="ophys_container_id") + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + df = enforce_df_column_order( + input_df=df, + column_order=VBO_METADATA_COLUMN_ORDER + ) + return df def _get_ophys_cells_table(self): """ @@ -427,8 +445,10 @@ def _get_ophys_cells_table(self): df = self.lims_engine.select(query) # NaN's for invalid cells force this to float, push to int - df["cell_specimen_id"] = pd.array( - df["cell_specimen_id"], dtype="Int64" + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True ) return df @@ -491,17 +511,28 @@ def get_ophys_session_table(self) -> pd.DataFrame: """ # There is one ophys_session_id from 2018 that has multiple behavior # ids, causing duplicates -- drop all dupes for now; # TODO - table = ( - self._get_ophys_session_table() - .drop_duplicates(subset=["ophys_session_id"], keep=False) - .set_index("ophys_session_id") + table = self._get_ophys_session_table().drop_duplicates( + subset=["ophys_session_id"], + keep=False + ) + # Make date time explicitly UTC. + table["date_of_acquisition"] = pd.to_datetime( + table["date_of_acquisition"], + utc=True ) # Fill NaN values of imaging_plane_group_count with zero to match # the behavior of the BehaviorOphysExperiment object. - im_plane_count = table["imaging_plane_group_count"].astype("Int64") - table["imaging_plane_group_count"] = im_plane_count - return table + table = enforce_df_int_typing( + input_df=table, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + table = enforce_df_column_order( + input_df=table, + column_order=VBO_METADATA_COLUMN_ORDER + ) + return table.set_index("ophys_session_id") def get_behavior_session( self, behavior_session_id: int @@ -530,9 +561,22 @@ def get_ophys_experiment_table(self) -> pd.DataFrame: :rtype: pd.DataFrame """ df = self._get_ophys_experiment_table() + df["date_of_acquisition"] = pd.to_datetime( + df["date_of_acquisition"], + utc=True + ) # Set type to pandas.Int64 to enforce integer typing and not revert to # float. - df["imaging_plane_group"] = df["imaging_plane_group"].astype("Int64") + df = enforce_df_int_typing( + input_df=df, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + df = enforce_df_column_order( + input_df=df, + column_order=VBO_METADATA_COLUMN_ORDER + ) + return df.set_index("ophys_experiment_id") def get_behavior_session_table(self) -> pd.DataFrame: @@ -547,13 +591,21 @@ def get_behavior_session_table(self) -> pd.DataFrame: acquisition date for behavior sessions (only in the stimulus pkl file) """ summary_tbl = self._get_behavior_summary_table() - # Query returns float typing of age_in_days. Convert to int to match - # typing of the Age data_object. - summary_tbl["age_in_days"] = summary_tbl["age_in_days"].astype("Int64") # Add UTC time zone to match timezone from DateOfAcquisition object. summary_tbl["date_of_acquisition"] = pd.to_datetime( summary_tbl["date_of_acquisition"], utc=True ) + # Query returns float typing of age_in_days. Convert to int to match + # typing of the Age data_object. + summary_tbl = enforce_df_int_typing( + input_df=summary_tbl, + int_columns=VBO_INTEGER_COLUMNS, + use_pandas_type=True + ) + summary_tbl = enforce_df_column_order( + input_df=summary_tbl, + column_order=VBO_METADATA_COLUMN_ORDER + ) return summary_tbl.set_index("behavior_session_id") diff --git a/allensdk/brain_observatory/ophys/project_constants.py b/allensdk/brain_observatory/ophys/project_constants.py index 5648f4f7a..f1d701399 100644 --- a/allensdk/brain_observatory/ophys/project_constants.py +++ b/allensdk/brain_observatory/ophys/project_constants.py @@ -52,33 +52,30 @@ } VBO_METADATA_COLUMN_ORDER = [ - "behavior_session_id", - "ophys_session_id", - "ophys_container_id", - "mouse_id", - "indicator", - "full_genotype", - "driver_line", - "cre_line", - "reporter_line", - "sex", - "age_in_days", - "imaging_depth", - "targeted_structure", - "targeted_imaging_depth", - "imaging_plane_group", - "project_code", - "session_type", + 'behavior_session_id', 'ophys_session_id', 'ophys_container_id', + 'mouse_id', 'indicator', 'full_genotype', 'driver_line', + 'cre_line', 'reporter_line', 'sex', 'age_in_days', + 'imaging_depth', 'targeted_structure', 'targeted_imaging_depth', + 'imaging_plane_group_count', 'imaging_plane_group', + 'project_code', 'session_type', 'session_number', 'image_set', + 'behavior_type', 'passive', 'experience_level', + 'prior_exposures_to_session_type', 'prior_exposures_to_image_set', + 'prior_exposures_to_omissions', 'date_of_acquisition', + 'equipment_name', 'num_depths_per_area', 'ophys_experiment_id', + 'num_targeted_structures', 'published_at', 'isi_experiment_id'] + + +VBO_INTEGER_COLUMNS = [ "session_number", - "image_set", - "behavior_type", - "passive", - "experience_level", - "prior_exposures_to_session_type", + "age_in_days", "prior_exposures_to_image_set", + "prior_exposures_to_session_type", "prior_exposures_to_omissions", - "date_of_acquisition", - "equipment_name", - "published_at", - "isi_experiment_id", + "ophys_session_id", + "imaging_plane_group_count", + "imaging_plane_group", + "targeted_areas", + "num_depths_per_area", + "num_targeted_structures", + "cell_specimen_id" ] diff --git a/allensdk/core/dataframe_utils.py b/allensdk/core/dataframe_utils.py index 8bf63814b..7dffde208 100644 --- a/allensdk/core/dataframe_utils.py +++ b/allensdk/core/dataframe_utils.py @@ -96,7 +96,8 @@ def patch_df_from_other( def enforce_df_column_order( - input_df: pd.DataFrame, column_order: List[str] + input_df: pd.DataFrame, + column_order: List[str] ) -> pd.DataFrame: """Return the data frame but with columns ordered. @@ -128,7 +129,9 @@ def enforce_df_column_order( def enforce_df_int_typing( - input_df: pd.DataFrame, int_columns: List[str], use_pandas_type=False + input_df: pd.DataFrame, + int_columns: List[str], + use_pandas_type: object = False ) -> pd.DataFrame: """Enforce integer typing for columns that may have lost int typing when combined into the final DataFrame. diff --git a/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py b/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py index e3c5d2968..ca46e387c 100644 --- a/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py +++ b/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py @@ -234,7 +234,6 @@ def _get_project_table_path(cls, fname): return cls.test_dir / 'ophys_cells_table.csv' @pytest.mark.requires_bamboo - @pytest.mark.skip('Skipping until data on s3 has been updated') def test_behavior_session_table(self): with patch.object( BehaviorMetadata, @@ -244,7 +243,7 @@ def test_behavior_session_table(self): from_lims = from_lims.drop(columns=list(SESSION_SUPPRESS)) from_s3 = self.cloud_cache.get_behavior_session_table() - from_s3 = from_s3.drop(columns=['file_id', 'isilon_filepath']) + from_s3 = from_s3.drop(columns=['file_id', 'file_path']) from_lims = from_lims.sort_index() from_s3 = from_s3.sort_index() @@ -252,7 +251,6 @@ def test_behavior_session_table(self): pd.testing.assert_frame_equal(from_lims, from_s3) @pytest.mark.requires_bamboo - @pytest.mark.skip('Skipping until data on s3 has been updated') def test_ophys_session_table(self): with patch.object( BehaviorMetadata, @@ -269,7 +267,6 @@ def test_ophys_session_table(self): pd.testing.assert_frame_equal(from_lims, from_s3) @pytest.mark.requires_bamboo - @pytest.mark.skip('Skipping until data on s3 has been updated') def test_ophys_experiments_table(self): with patch.object( BehaviorMetadata, @@ -281,7 +278,7 @@ def test_ophys_experiments_table(self): list(OPHYS_EXPERIMENTS_SUPPRESS_FINAL), errors='ignore') from_s3 = self.cloud_cache.get_ophys_experiment_table() - from_s3 = from_s3.drop(columns=['file_id', 'isilon_filepath']) + from_s3 = from_s3.drop(columns=['file_id', 'file_path']) from_lims = from_lims.sort_index() from_s3 = from_s3.sort_index() From fd138eaefc787c8f1a4faa084734ef884f86fca8 Mon Sep 17 00:00:00 2001 From: Christopher Morrison Date: Thu, 2 Nov 2023 17:58:24 -0700 Subject: [PATCH 2/4] Add section to vbo sphinx page. 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z5s=7+EVKp#_b5LTKm@7T^}LLdpADmJiTZ~iosT;!w{CQnJDe}_YD{WyCl70H#Wtzv zA0WCc9yX|G(OO;X*+`AIUe#bnw_#-~qSXRKMO@Yqy^qCMywuJIxRTRqfkpQlR?UQw zBMgE4o-Zk!?D4t0RpM8lW6^jK5s(`@$!DtOjp*HTqrDm$(K@gCh7a&=+_XcAHh}H{ z;nX%D{Y*t>`0reA)=^_$i75c;2npvQL#RA6Eiw|@x!)tfB6>!pZRqF>-h7r}a$eQA al)!pGVNc1W5b1t;S?P)DpM`QJZ~g}-x@&I$ literal 0 HcmV?d00001 diff --git a/doc_template/visual_behavior_optical_physiology.rst b/doc_template/visual_behavior_optical_physiology.rst index cacc12a50..fc3caaea3 100644 --- a/doc_template/visual_behavior_optical_physiology.rst +++ b/doc_template/visual_behavior_optical_physiology.rst @@ -229,6 +229,28 @@ unique imaging plane (**experiment**) that has been targeted on multiple recording days (**sessions**), under different behavioral and sensory conditions (**session types**). +SESSION STRUCTURE +----------------- + +During behavioral training, sessions consist of 60 minutes of change detection +behavior (other than the `TRAINING_0` sessions, which are 15 minutes of +associative reward pairing). + +During ophys sessions (`session_type` starting with `OPHYS`), there is a 5 +minute period where no stimulus is shown before the change detection task +begins, as well as 5 minutes of gray screen after the task ends. This allows +evaluation of spontaneous activity in the absence of stimulus or task. After +the second 5 minute gray screen period, a 30 second natural movie clip is +shown 10 times. This movie clip is the same as the Visual Coding 2P stimulus +called `natural_movie_one`. This allows evaluation of stimulus driven activity +that is independent of the task. + +Ophys session structure: + +.. image:: /_static/visual_behavior_2p/vbo_session_structure.png + :align: center + :width: 850 + DATA PROCESSING --------------- @@ -330,7 +352,9 @@ Removed data - Current data counts - 107 mice (same as v1.0.0) - 4079 behavior training session (down from 4082) - - 703 in vivo 2-photon imaging sessions (down from 705) + - 703 in vivo 2-photon imaging sessions (down from 704 sessions, + previous releases erroneously included session 875259383 in their + metadata tables and claimed 705 sessions.) - 50,476 logitudinal recordings (down from 50,489) Metadata Changes @@ -368,6 +392,7 @@ NWB Data Changes name containing "change_detection". Use the following example code snippet to retrieve the original stimulus block from the pandas table: stimulus_presentations[stimulus_presentations.stimulus_block_name.str.contains('change_detection')] + See "SESSION STRUCTURE" section above for more details. - New columns in the stimulus_presentations table: - is_image_novel, is_sham_change, movie_frame_index, movie_repeat, stimulus_block, stimulus_block_name, stimulus_name, active From 6fe632e896c2f980700a1ea95f61fbd8cba063ba Mon Sep 17 00:00:00 2001 From: Christopher Morrison Date: Thu, 2 Nov 2023 18:08:17 -0700 Subject: [PATCH 3/4] isort+black --- .../behavior_project_cache.py | 2 +- .../data_io/behavior_project_cloud_api.py | 29 +- .../data_io/behavior_project_lims_api.py | 53 ++-- .../ophys/project_constants.py | 47 +++- .../test_behavior_project_lims_api.py | 253 +++++++++++------- 5 files changed, 216 insertions(+), 168 deletions(-) diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py b/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py index 6b6aa2494..cea0a7125 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/behavior_project_cache.py @@ -74,7 +74,7 @@ class VBOLimsCache(Cache): class VisualBehaviorOphysProjectCache(ProjectCacheBase): PROJECT_NAME = "visual-behavior-ophys" - BUCKET_NAME = "visual-behavior-ophys-data" + BUCKET_NAME = "staging.visual-behavior-ophys-data" def __init__( self, diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py index 17652fa74..0365ef78c 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_cloud_api.py @@ -23,16 +23,16 @@ from allensdk.brain_observatory.behavior.behavior_session import ( BehaviorSession, ) +from allensdk.brain_observatory.ophys.project_constants import ( + VBO_INTEGER_COLUMNS, + VBO_METADATA_COLUMN_ORDER, +) from allensdk.core.dataframe_utils import ( enforce_df_column_order, enforce_df_int_typing, return_one_dataframe_row_only, ) from allensdk.core.utilities import literal_col_eval -from allensdk.brain_observatory.ophys.project_constants import ( - VBO_METADATA_COLUMN_ORDER, - VBO_INTEGER_COLUMNS -) COL_EVAL_LIST = ["ophys_experiment_id", "ophys_container_id", "driver_line"] @@ -190,13 +190,10 @@ def _get_ophys_session_table(self): df["date_of_acquisition"], utc="True" ) df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) df = enforce_df_column_order( - input_df=df, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=df, column_order=VBO_METADATA_COLUMN_ORDER ) self._ophys_session_table = df.set_index("ophys_session_id") @@ -223,13 +220,10 @@ def _get_behavior_session_table(self): df["date_of_acquisition"], utc="True" ) df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) df = enforce_df_column_order( - input_df=df, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=df, column_order=VBO_METADATA_COLUMN_ORDER ) self._behavior_session_table = df.set_index("behavior_session_id") @@ -261,13 +255,10 @@ def _get_ophys_experiment_table(self): df["date_of_acquisition"], utc="True" ) df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) df = enforce_df_column_order( - input_df=df, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=df, column_order=VBO_METADATA_COLUMN_ORDER ) self._ophys_experiment_table = df.set_index("ophys_experiment_id") diff --git a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py index b0a15a06a..dff7e491a 100644 --- a/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py +++ b/allensdk/brain_observatory/behavior/behavior_project_cache/project_apis/data_io/behavior_project_lims_api.py @@ -14,24 +14,24 @@ from allensdk.brain_observatory.ecephys.ecephys_project_api.http_engine import ( # noqa: E501 HttpEngine, ) +from allensdk.brain_observatory.ophys.project_constants import ( + VBO_INTEGER_COLUMNS, + VBO_METADATA_COLUMN_ORDER, +) from allensdk.core.auth_config import ( LIMS_DB_CREDENTIAL_MAP, MTRAIN_DB_CREDENTIAL_MAP, ) from allensdk.core.authentication import DbCredentials +from allensdk.core.dataframe_utils import ( + enforce_df_column_order, + enforce_df_int_typing, +) from allensdk.internal.api import db_connection_creator from allensdk.internal.api.queries.utils import ( build_in_list_selector_query, build_where_clause, ) -from allensdk.core.dataframe_utils import ( - enforce_df_column_order, - enforce_df_int_typing -) -from allensdk.brain_observatory.ophys.project_constants import ( - VBO_METADATA_COLUMN_ORDER, - VBO_INTEGER_COLUMNS -) class BehaviorProjectLimsApi(BehaviorProjectBase): @@ -394,13 +394,10 @@ def _get_ophys_experiment_table(self) -> pd.DataFrame: targeted_imaging_depth.columns = ["targeted_imaging_depth"] df = query_df.merge(targeted_imaging_depth, on="ophys_container_id") df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) df = enforce_df_column_order( - input_df=df, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=df, column_order=VBO_METADATA_COLUMN_ORDER ) return df @@ -446,9 +443,7 @@ def _get_ophys_cells_table(self): # NaN's for invalid cells force this to float, push to int df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) return df @@ -512,13 +507,11 @@ def get_ophys_session_table(self) -> pd.DataFrame: # There is one ophys_session_id from 2018 that has multiple behavior # ids, causing duplicates -- drop all dupes for now; # TODO table = self._get_ophys_session_table().drop_duplicates( - subset=["ophys_session_id"], - keep=False + subset=["ophys_session_id"], keep=False ) # Make date time explicitly UTC. table["date_of_acquisition"] = pd.to_datetime( - table["date_of_acquisition"], - utc=True + table["date_of_acquisition"], utc=True ) # Fill NaN values of imaging_plane_group_count with zero to match @@ -526,11 +519,10 @@ def get_ophys_session_table(self) -> pd.DataFrame: table = enforce_df_int_typing( input_df=table, int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + use_pandas_type=True, ) table = enforce_df_column_order( - input_df=table, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=table, column_order=VBO_METADATA_COLUMN_ORDER ) return table.set_index("ophys_session_id") @@ -562,19 +554,15 @@ def get_ophys_experiment_table(self) -> pd.DataFrame: """ df = self._get_ophys_experiment_table() df["date_of_acquisition"] = pd.to_datetime( - df["date_of_acquisition"], - utc=True + df["date_of_acquisition"], utc=True ) # Set type to pandas.Int64 to enforce integer typing and not revert to # float. df = enforce_df_int_typing( - input_df=df, - int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + input_df=df, int_columns=VBO_INTEGER_COLUMNS, use_pandas_type=True ) df = enforce_df_column_order( - input_df=df, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=df, column_order=VBO_METADATA_COLUMN_ORDER ) return df.set_index("ophys_experiment_id") @@ -600,11 +588,10 @@ def get_behavior_session_table(self) -> pd.DataFrame: summary_tbl = enforce_df_int_typing( input_df=summary_tbl, int_columns=VBO_INTEGER_COLUMNS, - use_pandas_type=True + use_pandas_type=True, ) summary_tbl = enforce_df_column_order( - input_df=summary_tbl, - column_order=VBO_METADATA_COLUMN_ORDER + input_df=summary_tbl, column_order=VBO_METADATA_COLUMN_ORDER ) return summary_tbl.set_index("behavior_session_id") diff --git a/allensdk/brain_observatory/ophys/project_constants.py b/allensdk/brain_observatory/ophys/project_constants.py index f1d701399..e9448feee 100644 --- a/allensdk/brain_observatory/ophys/project_constants.py +++ b/allensdk/brain_observatory/ophys/project_constants.py @@ -52,17 +52,40 @@ } VBO_METADATA_COLUMN_ORDER = [ - 'behavior_session_id', 'ophys_session_id', 'ophys_container_id', - 'mouse_id', 'indicator', 'full_genotype', 'driver_line', - 'cre_line', 'reporter_line', 'sex', 'age_in_days', - 'imaging_depth', 'targeted_structure', 'targeted_imaging_depth', - 'imaging_plane_group_count', 'imaging_plane_group', - 'project_code', 'session_type', 'session_number', 'image_set', - 'behavior_type', 'passive', 'experience_level', - 'prior_exposures_to_session_type', 'prior_exposures_to_image_set', - 'prior_exposures_to_omissions', 'date_of_acquisition', - 'equipment_name', 'num_depths_per_area', 'ophys_experiment_id', - 'num_targeted_structures', 'published_at', 'isi_experiment_id'] + "behavior_session_id", + "ophys_session_id", + "ophys_container_id", + "mouse_id", + "indicator", + "full_genotype", + "driver_line", + "cre_line", + "reporter_line", + "sex", + "age_in_days", + "imaging_depth", + "targeted_structure", + "targeted_imaging_depth", + "imaging_plane_group_count", + "imaging_plane_group", + "project_code", + "session_type", + "session_number", + "image_set", + "behavior_type", + "passive", + "experience_level", + "prior_exposures_to_session_type", + "prior_exposures_to_image_set", + "prior_exposures_to_omissions", + "date_of_acquisition", + "equipment_name", + "num_depths_per_area", + "ophys_experiment_id", + "num_targeted_structures", + "published_at", + "isi_experiment_id", +] VBO_INTEGER_COLUMNS = [ @@ -77,5 +100,5 @@ "targeted_areas", "num_depths_per_area", "num_targeted_structures", - "cell_specimen_id" + "cell_specimen_id", ] diff --git a/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py b/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py index ca46e387c..c2d7ea30b 100644 --- a/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py +++ b/allensdk/test/brain_observatory/behavior/behavior_project_cache/test_behavior_project_lims_api.py @@ -4,26 +4,26 @@ import pandas as pd import pytest - -from allensdk.brain_observatory.behavior.behavior_project_cache import \ - VisualBehaviorOphysProjectCache -from allensdk.brain_observatory.behavior.behavior_project_cache.project_metadata_writer.behavior_project_metadata_writer import ( # noqa: E501 - SESSION_SUPPRESS, OPHYS_EXPERIMENTS_SUPPRESS, - OPHYS_EXPERIMENTS_SUPPRESS_FINAL +from allensdk.brain_observatory.behavior.behavior_project_cache import ( + VisualBehaviorOphysProjectCache, ) from allensdk.brain_observatory.behavior.behavior_project_cache.project_apis.data_io import ( # noqa: E501 - BehaviorProjectLimsApi, BehaviorProjectCloudApi + BehaviorProjectCloudApi, + BehaviorProjectLimsApi, +) +from allensdk.brain_observatory.behavior.behavior_project_cache.project_metadata_writer.behavior_project_metadata_writer import ( # noqa: E501 + OPHYS_EXPERIMENTS_SUPPRESS, + OPHYS_EXPERIMENTS_SUPPRESS_FINAL, + SESSION_SUPPRESS, ) from allensdk.brain_observatory.behavior.data_objects import BehaviorSessionId -from allensdk.brain_observatory.behavior.data_objects.metadata\ - .behavior_metadata.behavior_metadata import \ - BehaviorMetadata -from allensdk.brain_observatory.behavior.data_objects.metadata\ - .behavior_metadata.session_type import \ - SessionType - -from allensdk.test_utilities.custom_comparators import ( - WhitespaceStrippedString) +from allensdk.brain_observatory.behavior.data_objects.metadata.behavior_metadata.behavior_metadata import ( # noqa: E501 + BehaviorMetadata, +) +from allensdk.brain_observatory.behavior.data_objects.metadata.behavior_metadata.session_type import ( # noqa: E501 + SessionType, +) +from allensdk.test_utilities.custom_comparators import WhitespaceStrippedString class MockQueryEngine: @@ -42,14 +42,18 @@ def stream(self, endpoint): @pytest.fixture def MockBehaviorProjectLimsApi(): - return BehaviorProjectLimsApi(MockQueryEngine(), MockQueryEngine(), - MockQueryEngine()) + return BehaviorProjectLimsApi( + MockQueryEngine(), MockQueryEngine(), MockQueryEngine() + ) @pytest.mark.parametrize( - "line,expected", [ - ("reporter", WhitespaceStrippedString( - """-- -- begin getting reporter line from donors -- -- + "line,expected", + [ + ( + "reporter", + WhitespaceStrippedString( + """-- -- begin getting reporter line from donors -- -- SELECT ARRAY_AGG (g.name) AS reporter_line, d.id AS donor_id FROM donors d LEFT JOIN donors_genotypes dg ON dg.donor_id=d.id @@ -57,9 +61,13 @@ def MockBehaviorProjectLimsApi(): LEFT JOIN genotype_types gt ON gt.id=g.genotype_type_id WHERE gt.name='reporter' GROUP BY d.id - -- -- end getting reporter line from donors -- --""")), - ("driver", WhitespaceStrippedString( - """-- -- begin getting driver line from donors -- -- + -- -- end getting reporter line from donors -- --""" + ), + ), + ( + "driver", + WhitespaceStrippedString( + """-- -- begin getting driver line from donors -- -- SELECT ARRAY_AGG (g.name) AS driver_line, d.id AS donor_id FROM donors d LEFT JOIN donors_genotypes dg ON dg.donor_id=d.id @@ -67,36 +75,42 @@ def MockBehaviorProjectLimsApi(): LEFT JOIN genotype_types gt ON gt.id=g.genotype_type_id WHERE gt.name='driver' GROUP BY d.id - -- -- end getting driver line from donors -- --""")) - ] + -- -- end getting driver line from donors -- --""" + ), + ), + ], ) -def test_build_line_from_donor_query(line, expected, - MockBehaviorProjectLimsApi): +def test_build_line_from_donor_query( + line, expected, MockBehaviorProjectLimsApi +): mbp_api = MockBehaviorProjectLimsApi assert expected == mbp_api._build_line_from_donor_query(line=line) class TestProjectTablesAll: """Tests for passing passed_only=False to project tables""" + @classmethod def setup_class(cls): - test_dir = Path(__file__).parent / 'test_data' / 'vbo' + test_dir = Path(__file__).parent / "test_data" / "vbo" # Note: these tables will need to be updated if the expected table # changes cls.release_behavior_sessions_table = pd.read_csv( - test_dir / 'behavior_session_table.csv') + test_dir / "behavior_session_table.csv" + ) cls.release_ophys_sessions_table = pd.read_csv( - test_dir / 'ophys_session_table.csv') + test_dir / "ophys_session_table.csv" + ) cls.release_ophys_experiments_table = pd.read_csv( - test_dir / 'ophys_experiment_table.csv') + test_dir / "ophys_experiment_table.csv" + ) cls.release_ophys_cells_table = pd.read_csv( - test_dir / 'ophys_cells_table.csv') - - cls.lims_api = BehaviorProjectLimsApi.default( - passed_only=False + test_dir / "ophys_cells_table.csv" ) + cls.lims_api = BehaviorProjectLimsApi.default(passed_only=False) + def _get_behavior_session(self, behavior_session_id, lims_db): if isinstance(behavior_session_id, BehaviorSessionId): behavior_session_id = behavior_session_id.value @@ -106,8 +120,8 @@ def _get_behavior_session(self, behavior_session_id, lims_db): behavior_session_id=BehaviorSessionId(behavior_session_id), equipment=None, stimulus_frame_rate=None, - session_type=SessionType('foo'), - behavior_session_uuid=None + session_type=SessionType("foo"), + behavior_session_uuid=None, ) @pytest.mark.requires_bamboo @@ -115,54 +129,78 @@ def test_all_behavior_sessions(self): """Tests that when passed_only=False, that more sessions are returned than in the release table""" with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): obtained = self.lims_api.get_behavior_session_table() # Make sure ids returned are superset of release ids - assert len(set(obtained.index).intersection( - self.release_behavior_sessions_table['behavior_session_id'])) \ - == \ - self.release_behavior_sessions_table['behavior_session_id']\ - .nunique() - assert obtained.shape[0] > \ - self.release_behavior_sessions_table.shape[0] + assert ( + len( + set(obtained.index).intersection( + self.release_behavior_sessions_table[ + "behavior_session_id" + ] + ) + ) + == self.release_behavior_sessions_table[ + "behavior_session_id" + ].nunique() + ) + assert ( + obtained.shape[0] + > self.release_behavior_sessions_table.shape[0] + ) @pytest.mark.requires_bamboo def test_all_ophys_sessions(self): """Tests that when passed_only=False, that more sessions are returned than in the release table""" with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): obtained = self.lims_api.get_ophys_session_table() # Make sure ids returned are superset of release ids - assert len(set(obtained.index).intersection( - self.release_ophys_sessions_table['ophys_session_id'])) \ - == \ - self.release_ophys_sessions_table['ophys_session_id']\ - .nunique() - assert obtained.shape[0] > \ - self.release_ophys_sessions_table.shape[0] + assert ( + len( + set(obtained.index).intersection( + self.release_ophys_sessions_table["ophys_session_id"] + ) + ) + == self.release_ophys_sessions_table[ + "ophys_session_id" + ].nunique() + ) + assert ( + obtained.shape[0] > self.release_ophys_sessions_table.shape[0] + ) @pytest.mark.requires_bamboo def test_all_ophys_experiments(self): """Tests that when passed_only=False, that more experiments are returned than in the release table""" with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): obtained = self.lims_api.get_ophys_experiment_table() # Make sure ids returned are superset of release ids - assert len(set(obtained.index).intersection( - self.release_ophys_experiments_table['ophys_experiment_id'])) \ - == \ - self.release_ophys_experiments_table['ophys_experiment_id']\ - .nunique() - assert obtained.shape[0] > \ - self.release_ophys_experiments_table.shape[0] + assert ( + len( + set(obtained.index).intersection( + self.release_ophys_experiments_table[ + "ophys_experiment_id" + ] + ) + ) + == self.release_ophys_experiments_table[ + "ophys_experiment_id" + ].nunique() + ) + assert ( + obtained.shape[0] + > self.release_ophys_experiments_table.shape[0] + ) @pytest.mark.requires_bamboo def test_all_cells(self): @@ -171,41 +209,47 @@ def test_all_cells(self): obtained = self.lims_api.get_ophys_cells_table() # Make sure ids returned are superset of release ids - assert len(set(obtained['ophys_experiment_id']).intersection( - self.release_ophys_cells_table['ophys_experiment_id'])) \ - == \ - self.release_ophys_cells_table['ophys_experiment_id']\ - .nunique() - assert obtained.shape[0] > \ - self.release_ophys_cells_table.shape[0] + assert ( + len( + set(obtained["ophys_experiment_id"]).intersection( + self.release_ophys_cells_table["ophys_experiment_id"] + ) + ) + == self.release_ophys_cells_table["ophys_experiment_id"].nunique() + ) + assert obtained.shape[0] > self.release_ophys_cells_table.shape[0] class TestLimsCloudConsistency: """Tests for checking consistency between tables as returned by cloud vs those returned internally by lims""" + @classmethod def setup_class(cls): tempdir = tempfile.TemporaryDirectory() - cls.test_dir = Path(__file__).parent / 'test_data' / 'vbo' + cls.test_dir = Path(__file__).parent / "test_data" / "vbo" behavior_sessions_table = pd.read_csv( - cls.test_dir / 'behavior_session_table.csv') - cls.session_type_map = ( - behavior_sessions_table - .set_index('behavior_session_id')[['session_type']] - .to_dict()['session_type']) + cls.test_dir / "behavior_session_table.csv" + ) + cls.session_type_map = behavior_sessions_table.set_index( + "behavior_session_id" + )[["session_type"]].to_dict()["session_type"] cls.lims_cache = VisualBehaviorOphysProjectCache.from_lims( - data_release_date=['2021-03-25', '2021-08-12']) + data_release_date=["2021-03-25", "2021-08-12"] + ) cls.tempdir = tempdir with patch.object( - BehaviorProjectCloudApi, - '_get_metadata_path', - wraps=cls._get_project_table_path): + BehaviorProjectCloudApi, + "_get_metadata_path", + wraps=cls._get_project_table_path, + ): cls.cloud_cache = VisualBehaviorOphysProjectCache.from_s3_cache( - cache_dir=tempdir.name) + cache_dir=tempdir.name + ) def teardown_class(self): self.tempdir.cleanup() @@ -218,32 +262,33 @@ def _get_behavior_session(self, behavior_session_id, lims_db): equipment=None, stimulus_frame_rate=None, session_type=SessionType( - self.session_type_map[behavior_session_id]), - behavior_session_uuid=None + self.session_type_map[behavior_session_id] + ), + behavior_session_uuid=None, ) @classmethod def _get_project_table_path(cls, fname): - if fname == 'behavior_session_table': - return cls.test_dir / 'behavior_session_table.csv' - elif fname == 'ophys_session_table': - return cls.test_dir / 'ophys_session_table.csv' - elif fname == 'ophys_experiment_table': - return cls.test_dir / 'ophys_experiment_table.csv' - elif fname == 'ophys_cells_table': - return cls.test_dir / 'ophys_cells_table.csv' + if fname == "behavior_session_table": + return cls.test_dir / "behavior_session_table.csv" + elif fname == "ophys_session_table": + return cls.test_dir / "ophys_session_table.csv" + elif fname == "ophys_experiment_table": + return cls.test_dir / "ophys_experiment_table.csv" + elif fname == "ophys_cells_table": + return cls.test_dir / "ophys_cells_table.csv" @pytest.mark.requires_bamboo def test_behavior_session_table(self): with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): from_lims = self.lims_cache.get_behavior_session_table() from_lims = from_lims.drop(columns=list(SESSION_SUPPRESS)) from_s3 = self.cloud_cache.get_behavior_session_table() - from_s3 = from_s3.drop(columns=['file_id', 'file_path']) + from_s3 = from_s3.drop(columns=["file_id", "file_path"]) from_lims = from_lims.sort_index() from_s3 = from_s3.sort_index() @@ -253,8 +298,8 @@ def test_behavior_session_table(self): @pytest.mark.requires_bamboo def test_ophys_session_table(self): with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): from_lims = self.lims_cache.get_ophys_session_table() from_lims = from_lims.drop(columns=list(SESSION_SUPPRESS)) @@ -269,16 +314,18 @@ def test_ophys_session_table(self): @pytest.mark.requires_bamboo def test_ophys_experiments_table(self): with patch.object( - BehaviorMetadata, - 'from_lims', wraps=self._get_behavior_session): + BehaviorMetadata, "from_lims", wraps=self._get_behavior_session + ): from_lims = self.lims_cache.get_ophys_experiment_table() from_lims = from_lims.drop( - columns=list(OPHYS_EXPERIMENTS_SUPPRESS) + - list(OPHYS_EXPERIMENTS_SUPPRESS_FINAL), errors='ignore') + columns=list(OPHYS_EXPERIMENTS_SUPPRESS) + + list(OPHYS_EXPERIMENTS_SUPPRESS_FINAL), + errors="ignore", + ) from_s3 = self.cloud_cache.get_ophys_experiment_table() - from_s3 = from_s3.drop(columns=['file_id', 'file_path']) + from_s3 = from_s3.drop(columns=["file_id", "file_path"]) from_lims = from_lims.sort_index() from_s3 = from_s3.sort_index() From 77737ecbc0efe769305f884d5ebd47858cf4b5a5 Mon Sep 17 00:00:00 2001 From: Christopher Morrison Date: Fri, 3 Nov 2023 10:00:21 -0700 Subject: [PATCH 4/4] Update more docs --- doc_template/visual_behavior_optical_physiology.rst | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/doc_template/visual_behavior_optical_physiology.rst b/doc_template/visual_behavior_optical_physiology.rst index fc3caaea3..476079ed4 100644 --- a/doc_template/visual_behavior_optical_physiology.rst +++ b/doc_template/visual_behavior_optical_physiology.rst @@ -16,6 +16,9 @@ data access and subsequent analysis. No knowledge of the NWB file format is requ Specific information about how Visual Behavior Optical Physiology data is stored in NWB files and how AllenSDK accesses NWB files can be found `here `_. +TUTORIALS +-------------------------------------------- + To get started, check out these jupyter notebooks to learn how to: 1) `Download data using the AllenSDK or directly from our Amazon S3 bucket <_static/examples/nb/visual_behavior_ophys_data_access.html>`_ `(download .ipynb) <_static/examples/nb/visual_behavior_ophys_data_access.ipynb>`_ `(Open in Colab) `_ @@ -24,6 +27,10 @@ To get started, check out these jupyter notebooks to learn how to: 4) `Examine the full training history of one mouse <_static/examples/nb/visual_behavior_mouse_history.html>`_ `(download .ipynb) <_static/examples/nb/visual_behavior_mouse_history.ipynb>`_ `(Open in Colab) `_ 5) `Compare behavior and neural activity across different trial types in the task <_static/examples/nb/visual_behavior_compare_across_trial_types.html>`_ `(download .ipynb) <_static/examples/nb/visual_behavior_compare_across_trial_types.ipynb>`_ `(Open in Colab) `_ +DOCUMENTATION +-------------------------------------------- + +To learn more about the experimental design and available data, read through the Visual Behavior and Visual Behavior Ophys chapters in the `SWDB Databook `_. For a description of available AllenSDK methods and attributes for data access, see this `further documentation `_.

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